eEF1A1 coordinates different levels of HSP70 expression during heat stress to promote cell survival

eEF1A1 is a regulator of the adaptation to heat stress
1. Fig1a: eEF1A1 knockdown reduces Hsp70 expression during heat stress
2. Fig1b: eEF1A1 knockdown reduces cell survival during heat stress
eEF1A1 is a specific transcriptional regulator of HSP70 expression
1. Fig1c: eEF1A1 knockdown reduces Hsp70 mRNA expression during heat stress
2. Fig1d: eEF1A1 knockdown reduces RNA Pol II occupancy of an HSP70 gene (but not of an unrelated control gene)
3. Fig1e/f: eEF1A1 knockdown reduces the number and intensity of HSP70 transcription sites
eEF1A1 regulates HSF1 activity in transcriptionally active sites during heat stress, by interacting with it in a promoter-bound complex
1. eEF1A1 regulates HSF1 activity by interacting with it in a promoter-bound complex
  1. eEF1A1 regulates HSF1 activity by interacting with it
    1. eEF1A1 regulates HSF1 activity
      1. Fig2a: eEF1A1 knockdown reduces binding of HSF1 to a synthetic HSP70 promoter fragment
      2. Fig2b: eEF1A1 knockdown reduces HSF1 promoter occupancy in vivo
    2. Fig2c: eEF1A1 interacts with HSF1 in vivo
    3. Fig2d: eEF1A1-HSF1 complexes bind to a synthetic HSP70 promoter fragment
  2. Fig2f: eEF1A1 itself binds to synthetic HSP70 promoter DNA
  3. Fig2e: eEF1A1 specifically binds to HSP70 promoters in vitro
  4. Fig3a-c: eEF1A1 localises to HSP70 transcription sites during heat stress in vivo
2. eEF1A1 is recruited to transcriptionally active sites during heat stress
  1. Fig3d: eEF1A1 binding to HSP70 transcription sites is dependent on ongoing transcription
  2. Fig3e: eEF1A1 directly binds to RNA pol II
eEF1A1 controls HSP70 translation by binding to it
1. Fig5d: eEF1A1 colocalises with HSP70 mRNA in the cytoplams during heat stress
2. Fig5e: eEF1A1 knockdown reduces HSP70 mRNA translation during heat stress
Fig5a-c: eEF1A1 knockdown impairs HSP70 mRNA export
eEF1A1 promotes HSP70 mRNA stability by binding to it
1. eEF1A1 binds to HSP70 mRNA
  1. Fig4a,b: eEF1A1 remains bound to HSP70 mRNA following transcription
  2. Fig4c: eEF1A1 binds to the 3'-UTR of HSP70 mRNA in vitro
2. eEF1A1 controls HSP70 mRNA stability
  1. Fig4e: eEF1A1 knockdown reduces HSP70 mRNA half-life
  2. Fig4f: eEF1A1 knckdown reduces the expression of a reporter gene containing the HSP70 promoter and 3'-UTR

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eEF1A1 coordinates different levels of HSP70 expression during heat stress to promote cell survival

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	Erstellt von Tobias von der Haar vor etwa 10 Jahre