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Histology

Beschreibung

Histology Stains , Microscopes, Collagen etc
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Zusammenfassung der Ressource

Histology
  1. Light microscopy
    1. Formalyn, paraffin embedding
    2. TEM
      1. Shows interior of the Cell
        1. Glutaraldehyde and osmium tetroxide
      2. SEM
        1. Specimen is sputter coated with silver
          1. glutaraldehyde
        2. Fluorescent Microscopy
          1. Immunohistochemistry
            1. substrate or flourescence
          2. Hematoxylin and Eosin
            1. Most Common
              1. Hematoxylin stains Nucleic acids & ER Blue
                1. Neurons - Basophilic Nissl bodies in cytoplasm (rER)
                2. Eosin stains (acidic) mitochondria Hemoglobin, Elastic Fibers red/pink
                3. Periodic acid-Schiff (PAS)
                  1. Stain for glycoproteins Sugar
                    1. MicroVilli (Glycocalyx)
                    2. Basal lamina (Laminin) Red / Pink / Magenta
                      1. Type IV Collagen
                    3. Trichrome Blue
                      1. Collagen Type I
                      2. Silver Stain
                        1. Reticular Fibers
                          1. Type III
                            1. Brown / Black
                            2. Nerve Fibers
                              1. Brown / Black
                            3. Bright Field
                              1. Light beam passes through stained specimen
                              2. Hyaline (grape soda)
                                1. Type II - Basophilic
                                  1. Aggrecan Hyaluronic Acid
                                    1. Chondroitin Sulfate
                                      1. A lot of smooth extra cellular matrix between cells
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