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CAP and cAMP
- CAP
- Sequence specific DNA binding protein
- cyclic adenosine monophosphate
- stabilizes the polymerase with the help of cAMP
- Sequence specific DNA binding protein
- cAMP
- CAP only binds the DNA when its bound to cAMP
- helps to stabilize the RNAP on the promoter of DNA
- helps to stabilize the RNAP on the promoter of DNA
- when glucose levels are high cAMP levels are low
- dont need to stabilize the polymerase for transcription if have glucose already
- dont need to stabilize the polymerase for transcription if have glucose already
- CAP only binds the DNA when its bound to cAMP
- level examples
- low glucose, low lactose
- cAMP high and stabilizes polymerase, repressor bound so little transcription
- cAMP high and stabilizes polymerase, repressor bound so little transcription
- low glucose, high lactose
- cAMP high, high transcription
- cAMP high, high transcription
- high glucose, high lactose
- cAMP low, RNAP not stabilized, transcription occurs at a low level
- cAMP low, RNAP not stabilized, transcription occurs at a low level
- high glucose, low lactose
- low cAMP, RNAP not stabilized, repressor, not much transcription
- low cAMP, RNAP not stabilized, repressor, not much transcription
- low glucose, low lactose
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