Zusammenfassung der Ressource
reporter genes
- report levels of expression
- GFP (green flurescent protein) and luciferase
- two examples of reporter genes
- visually show expression
- GOI (gene of interest)
- when and where in the development is the gene expressed?
- to find out "hook it up" to a reporter gene
- IPTG
- triggers transcription of the lac operon
- analog of lactose