CELL CULTURE

CELL CULTURE

PREPARATION OF MEDIUM FORMS
1. CONCENTRATE
2. WORKING
3. POWERED
COMPOSITION AND PROPERTIES OF THE CULTURE MEDIUM
1. MINERAL SALS
2. GLUCOSE
3. VITAMINS
4. TAMPON
5. ORGANICS SUPLEMENTS, LOW MOLECULAR WEIGHT
6. HORMONES GROWTH FACTORS
  1. = ANIMALS SERUM
    1. + PROTEINES
7. ANTIBIOTICS ANTIFUNGALS
CRITERIA FOR SELECTING MEDIA
1. CELL LINES
  1. THE CHOICE OF CELL CULTURE MEDIAL IS EXTREMELLY IMPORTANT & SIGNIFICANTLY AFFECTS THE SUCCES OF CELL CULTURE EXPERIMENS
2. PRIMARY CELL CULTURE
  1. PRIVIDES UNIQUE & VALUABLE RESEARCH DATA
  2. COMPLETE & CONDICIONED MEDIUM
    1. REDUCES
      1. RISK OF CONTAMINATION
      2. TIME
      3. LABOR
      4. MONEY
COMMON CELL CULTURE MEDIA
1. EAGLE´S MINIUM ESSENTIAL MEDIUM (EMEM)
  1. CONTAINS
    1. SODIUM PYRUVATE
    2. NONESSENTIAL AMINO ACIDS
    3. SAL SOLUTION
  2. NON-COMPLEX MEDIUM
2. RPMI-1640
  1. GENERAL PURPOSE MEDIA
    1. MAMMALIAN CELSS
      1. HEMATOPOIETIC CELSS
      2. HUMAN LYMPHOCITIES FUSION PROTOCOLS CROWTH OF HYBRID CELLS
3. DUBLECCO´S MODIFIED EAGLE´S MEDIUM (DMEM)
  1. CONCENTRATIOM
    1. AMINO ACIDS
    2. VITAMINES
  2. BASAL MEDIUM
    1. WITH SUMPLEMENTATION
      1. COMPLETE MEDIUM
4. DMEM/F-12
  1. MIXTURE OF DMEM & HAM´S F-12
    1. RICH MEDIUM
      1. COMPLEX MEDIUM
    2. CELLS TYPES IN BOTH SERUM
5. ISCOVE´S MODIFIED DULBECCO´S MEDIUM (IMDM)
  1. CONTAINING
    1. SELENIUM
    2. AMINO ACIDS
    3. VITAMINS
    4. INORGANIC SALT
    5. HEPES
    6. POTASIIUM NITRATE
    7. SODIUM PYRUVATE
  2. GROWTH OF LYMPHOCYTES & BYBRIDOMAS
6. HAM´S NUTRIENT MIXTURES
  1. F-10 HAM
    1. GROWTH OF HUMAN DIPLOID CELLS & WHITE BOOD CELLS
  2. F-12 HAM
    1. GROWTH OF PRIMARY RAT HEPATOCITES RAT PROSTATE EPITHELIAL CELSS
  3. COON F-12 HAM
    1. CULTURING HYBRID CELLS
    2. CONTAIN
      1. DOBLE AMINO ACIDS & PIRUVATE
        F-12 HAM
      2. ASCORBIC ACID
INCUBATION CONDITIONS
1. Tª
  1. 28Cº INSECTS 35Cº ANIMALS
2. HUMIDITY 95%
FEATURES PHYSIOCHEMICAL CULTURE MEDIUM
1. PH 7,2-7.6
2. OSMOLARITY 200-320 OSM/KG
3. VISCOSITY
4. SURFACE TENSION
GAS ATMOSPHERE
1. WERE GAS MIX CULTURES ARE MAINTAINED
2. COMPONENTS
  1. OXYGEN
  2. CO2

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	Erstellt von Olaia Gorriz vor etwa 9 Jahre