Zusammenfassung der Ressource
Prokaryotic Diversity and Classification
- What is prokaryote?
- Archea
- similar shape/size
- multiply by binary fission
- may have flagella
- no peptidoglycan in cell wall
- many are extremophiles
- Hyperthermophile (over 100 degree celcius)
- Pyrobolus
- Halophiles (can grow in high salt medium)
- Halobacterium *eventho it's actually not a bacterium*
- Acidophiles (can grow in acid condition, best at pH 2)
- Thermoplasma
- Bacteria
- Classifying prokaryotes
- Morphological classification
- Shape
- coccus (round shape)
- rod
- spirillum (spiral shape)
- spirochete (pasta-like)
- budding & appendaged bacteria
- filamentous bacteria
- Arrangement
- Cell size & significance of smallness
- 0.2 micrometres - 700 nanometres diameter
- upper size limit
- due to surface area to volume ratio for absoprtion of gases & nutrients
- lower size limit
- due to space needed to house essential proteins, nucleic acids etc
- Biochemical classification
- by using API kit (however it takes a long time to show the result)
- Phylogenetics classification
- Bacterial 16s rRNA genes
- use to identify bacteria
- highly variable among species
- most of the bacteria have it!
- Phylogenetic Overview of Bacteria
- Isolate /amplify 16s RNA genes
- sequence DNA and align sequences
- construct the phylogenetic tree
- Identifying and classifying microbial species
- Binomial system of nomenclature
- genus name
- species name
- Strain
- Species
- Basic concept of species on eukaryotes
- Concept of species on microbes
- Microbial classification
- Name my own bacteria by writing a paper to International Journal of Systematic and Evolutionary Microbiology
- The paper is published by them
- I must deposit my organism in at least two international culture collections
- ATCC (USA)
- DSMZ (Germany)
- JCM (Japan)