Gen Lec. Note 10/23 (Chapter 11)

Semiconservative is the method of DNA replication, how does it work?

This method of DNA replication is intermixing of parental and daughter strands (hybrid).

In order ____ complementary strands, the ______ _____ helix is used as a ______

Newly-formed DNA strands are called?

The original (2) 2x helix strands are called?

The conservation model was (1) of proposed methods of DNA replication, what is it?

This model was proposed in the 50's + proved to be correct, what is it?

What experiment proved that DNA replication is semiconservative?

DNA polymerase needs what (3) components to make new daughter strands?

As the chain of DNA elongates, what direction is the replication going?

What type of nucleotides are added in DNA replication?

What happens as a nucleotide is added?

DNA polymerase I + ____ needs a RNA ____ to start added _____.

Prokar. have (_) orgins of replication + replication is in what direction?

Name steps of prokar. chromos. replication

The enzy. starting the production of new DNA

DNA polymerase needs a DNA ____ to add ____ @ 3' -___ of an ______ ________.

Polymerase _ in prokar. removes RNA primers + these subsequent gaps.

Polymerase _ in prokar.s works from _' to _' + makes edits after ______.

How does the DNA polymerase work in prokar.s?

____ make the initial split in DNA.

These helicases helps smooth out coils @ end of replication forks, breaks H-bond between DNA strands + further open strands.

Single-stranded binding proteins do what?

DNaB + DNaC are both made of what?

Why does DNA gyrase goes before helicases?

Once DNA strands are separated, what is made next?

Who makes the RNA primers?

RNA primers are how long?

This daughter strand is made from 5' to 3' direction.

This strand is opposite to the leading strand in DNA synthesis.

What is the lagging strand made of?

After RNA primers are removed, what are they filled w/ + by whom?

DNA polymerase 3 is also knowns as a...?

What is 5' to 3' exonuclease activity?