Practical 1

Why is mannitol salt agar used to select for normal skin flora?

What is mannitol salt agar used for? Which microbe.

After you have observed a Gram-positive coccus, you need additional information before performing a coagulase test. What is this additional information?

If a bacteria is gram positive, catalase positive and co-agulase positive. What is it?

If a bacteria is gram positive, catalase positive and produces acid from mannose. What is it?

If a bacteria is gram positive, catalase positive and produces no acid from frutose. What is it?

List three identifying characteristics of S. aureus.

List 4 factors that protect the skin from infection.

What is coagulase? How is it related to pathogenicity of Staphylococci?

When doing a skin culture, why is the swab first moistened with saline?

What test can differentiate the three major species of Staphylococcus? List 4.

How could you distinguish S. epidermidis from S. saprophyticus in the Micrococcaceae identification Key?

Why are staphylococcal infections frequent among hospital patients? 3 reasons.

What is Vogel Johnson Agar and what is it used for?
Species
Example
Samples
Composition

What is the catalase test?

What is the coagulase test for?
What microbe tests positive?

List 5 identifying characteristics of S. epidermidis

List 6 identify characteristics of S. saprophyticus

List 4 tests that distinguish major species of Staphylococci

What is the DNase test and what is a positive result.

What is the colony pigment test? How does it work?

What is the Staphlyococci Staph 12s kit do? How does it do it?

Why is blood agar a differential media for Streptococci?

Is gram staining results of significant importance for identification of microorganisms from the throat.

A gram-positive cocci has been found that cannot be identified as Staph or Strep. What enzyme test will quickly differentiate between these two bacteria?

What type of haemolysis is produced by S. pneumoniae?

What type of haemolysis is produced by S. pyogenes?

How do you determine is a sore throat is caused by S. pyogenes or S. pneumoniae?

List the names of 4 types of diseases caused by S. pyrogenes.

How would you differentiate between alpha and beta haemolysis?

What does the Optichin test illustrate?

What are the 3 types of haemolysis and what are their patterns?

Which streptococci are considered most commonly pathogenic?

What are the 4 main microflora in the throat/upper respiratory tract?

Why is infection rare in the upper respiratory tract?

What species causes acute pharyngitis?

What is the bacitracin Test?