What is the purpose of Coombs control cells?
To ensure that AHG tests with negative results are not the false negatives
To ensure that washing removed all unbound antibody
To ensure that AHG was not omitted or inactivated
All of the above
What is a positive DAT?
In vitro sensitization of RBC with antigen
In vitro sensitization of RBC with antibody
In vivo sensitization of RBC with antibody
In vivo sensitization of RBC with antigen
A positive autocontrol in antibody detection procedures is usually indicative of:
Inadequate washing
Positive DAT
Positive IAT
two of the above
Why is it important to match the lot number on the panel cells?
The ABO group will change from lot to lot
Pattern of reactions will change from lot to lot
It is a requirement of the FDA
In interpreting an antibody screen, which of the following questions might be asked to decipher the class of antibody?
Is the autologous control positive or negative?
Is hemolysis present?
In what phase did the reaction occur?
Is rouleaux present?
What is an elution?
a technique used to dissociate IgM antibodies from sensitized RBCs
a technique used to dissociate IgG antibodies from sensitized RBCs
a technique used to reduce the zeta potential enhancing antigen binding
none of the above
What is tested in an antibody screen?
Patient red cells are tested against group O reagent screening cells
Patient serum is tested against group AB reagent screening cells
Patient serum is tested against group O reagent screening cells
Patient serum is tested against group A reagent screening cells
What might a positive antibody screen and a negative autocontrol indicate?
An alloantibody is coating donor cells after a transfusion
An autoantibody has been detected
An alloantibody has been detected
Drug-induced antibody reacting with patient cells.
When might you suspect multiple antibodies in a patient's serum?
Pattern of reactivity not fitting a single antibody
Variation in phase of reactivity
Variation in antibody reactivity strength
One drop of Coombs control cells was added to a negative antibody screen. No agglutination was observed after centrifugation. What course of action is taken?
Report negative result
Report inconclusive result
Repeat the test
Add one more drop of check cells and recentrifuge
What is the shipping temp requirement for plasma?
1 to 6 degree F
1 to 6 degree C
-18 or higher degree F
-18 or lower degree C
Which of the following may not be used as a patient identifier?
Patient's full name
Patients date of birth
Patients medical record number
Patients room number
Which of the following is not an enhancement media?
Albumin
Low Ionic Strength Solution (LISS)
Normal Saline
Polyethylene glycol
Additive solutions are approved for storage of red blood cells for how many days?
21
42
35
7
What is the current storage time and storage temp for platelet concentrates and apheresis platelet components?
5 days at 1 to 6 degrees C
5 days at 24 to 27 degrees C
5 days at 20 to 24 degrees C
7 days at 22 to 24 degrees C
Whole blood and RBC units are stored at what temperature?
1 to 6 C
20 to 24 C
37 C
24 to 27 C
