Flash cards - a simple overview of the proteomics basics.
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Erstellt von Dominique M
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Erstellt von Dominique M
vor mehr als 5 Jahre
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What is the consequence of a block at the start of the citric acid cycle knowing that involved proteins have strong epistatic interactions?
Explain the relationship between 'linear dependence' and 'the ribosome'
Argue why it can be complicated to find a certain protein.
What makes detection of all proteins at the same time difficult?
What are techniques to determine protein structure?
What is the principle of X-ray crystallography?
What is the principle of NMR?
What is a benefit?
What is the principle of single particle EM?
Which two MS techniques are most used?
What is the principle of ESI-MS?
What is the principle of MALDI-TOF?
What is the difference is mass of various isotopes?
Can a protein be identified based on its mass only? What is the solution?
Name the steps for protein identification with mass spectrometry.
Why are double charged peptides preferred?
What is the name of the ions generated when a peptide is broken at its peptide bond?
Name the use of the quadrupole mass analyzer.
We end up with b-ions and y-ions. What do the 'b' and 'y' part refer to?
What do the numbers indicate in the following situation?
b2, y2
Peptide identification is difficult. Name an approach other than de novo identification.
You have a sample with 6000 proteins which vary in copy number. Which technique is appropriate?
