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Erstellt von Dominique M
vor mehr als 5 Jahre
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A protein is broken into peptides. You measure - your mass spectrometer has a mass tolerance of 1 Da. You find 500 hits, which it too much. What is your solution?
Is it adequate to use one peptide for identification of a protein with many repeats?
What is comparative genomics?
What is the principle behind SILAC?
What else can SILAC be used for (relative)?
What else can SILAC be used for (absolute)?
Advantages of SILAC
Disadvantages of SILAC
What is the procedure for Label Free Quantification (LFQ)?
Advantages of LFQ?
Disadvantages of LFQ?
How can PTM be identified when the peak height is very low?
If there are multiple S/T/Y amino acids in a row, it is almost impossible to determine on which of the AA the modification is. What is a way to do so?