Charlotte Jakes
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Cell Biology and Signalling Quiz am Protein Targeting, erstellt von Charlotte Jakes am 04/01/2020.

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Charlotte Jakes
Erstellt von Charlotte Jakes vor fast 5 Jahre
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Protein Targeting

Protein Targeting

Frage 1 von 39

1

What sequence on a protein helps us target to the endoplasmic reticulum?

Wähle eine der folgenden:

  • Signal sequence

  • Nuclear localisation sequence

  • SNARE sequence

  • Glucokinase

Erklärung

Frage 2 von 39

1

The signal sequence is the first part of a ER-targeted protein to be synthesised.

Wähle eins der folgenden:

  • WAHR
  • FALSCH

Erklärung

Frage 3 von 39

1

At which peptide terminal is the signal sequence found?

Wähle eine der folgenden:

  • N-terminal

  • C-terminal

Erklärung

Frage 4 von 39

1

The signal sequence codes for a series of hydrophilic amino acids.

Wähle eins der folgenden:

  • WAHR
  • FALSCH

Erklärung

Frage 5 von 39

1

What molecule binds to a protein-ribosome complex and facilitates binding to a receptor on the endoplasmic reticulum?

Wähle eine der folgenden:

  • Signal Recognition Particle (SRP)

  • Nuclear Localisation Sequence (NLS)

  • SNARE protein

  • HSP70

Erklärung

Frage 6 von 39

1

Through what protein is the new protein guided through after it has been targeted to the endoplasmic reticulum?

Wähle eine der folgenden:

  • Translocon channel

  • Signal Recognition Particle

  • SNARE

  • Na+/K+ ATPase

Erklärung

Frage 7 von 39

1

Fülle die Lücken, um den Text zu vervollständigen.

Fill in the blanks below to describe protein targeting to the endoplasmic reticulum.
1. The first part of the protein synthesised is the . This is a series of amino acids at the -terminus of the peptide.
2. The is recognised by (SRP).
3. is recognised by a receptor on the ER membrane.
4. The is cleaved off, leaving the bound to the ER membrane.
5. The protein is guided through a t protein on the ER membrane as its synthesis continues.
6. The enzyme cleaves the signal sequence off the protein once synthesis is complete.

Erklärung

Frage 8 von 39

1

From which face of the golgi are vesicles budded off for transport?

Wähle eine der folgenden:

  • Cis

  • Trans

Erklärung

Frage 9 von 39

1

At which face of the golgi are vesicles received from transport?

Wähle eine der folgenden:

  • Cis

  • Trans

Erklärung

Frage 10 von 39

1

SNARE proteins facilitate the targeting of what?

Wähle eine der folgenden:

  • Vesicles

  • ER proteins

  • Golgi apparatus proteins

  • Nuclear proteins

Erklärung

Frage 11 von 39

1

When are v-SNARES incorporated into the membranes of vesicles?

Wähle eine der folgenden:

  • During budding

  • During phospholipid synthesis

  • During targeting

  • In the cytosol

Erklärung

Frage 12 von 39

1

What type of SNARE proteins are present at the target membranes where they will be complementary to a specific v-SNARE?

Wähle eine der folgenden:

  • t-SNARE

  • m-SNARE

  • f-SNARE

  • p-SNARE

Erklärung

Frage 13 von 39

1

When does mitochondrial targeting occur?

Wähle eine der folgenden:

  • After translation before folding

  • After folding

Erklärung

Frage 14 von 39

1

At which terminus of a mitochondrial-targeted protein will you find the matrix-targeting sequence?

Wähle eine der folgenden:

  • N-terminus

  • C-terminus

Erklärung

Frage 15 von 39

1

What protein binds to mitochondrial-targeted proteins in the cytosol and matrix to prevent them from folding?

Wähle eine der folgenden:

  • HSP70 chaperone

  • TIM44

  • TOM40

  • TIM23/17

Erklärung

Frage 16 von 39

1

The matrix targeting sequence binds to what?

Wähle eine der folgenden:

  • Import receptor on outer membrane

  • TOM40

  • TIM44

  • HSP70 chaperone

Erklärung

Frage 17 von 39

1

What import pore is the mitochondrial protein targeted into first?

Wähle eine der folgenden:

  • TOM40

  • TIM23/17

  • TIM44

Erklärung

Frage 18 von 39

1

Cytosolic HSP70 is cleaved from the mitochondrial protein as it enters the first import pore.

Wähle eins der folgenden:

  • WAHR
  • FALSCH

Erklärung

Frage 19 von 39

1

What is required to cleave HSP70 chaperone from mitochondrial proteins?

Wähle eine der folgenden:

  • ATP hydrolysis

  • GTP hydrolysis

  • ATP synthesis

  • Coenzyme A

Erklärung

Frage 20 von 39

1

What is the function of matrix processing protease?

Wähle eine der folgenden:

  • Cleaves matrix-targeting sequence from protein

  • Cleaves HSP70 chaperone from protein

  • Activates TOM40

  • Inactives TIM44

Erklärung

Frage 21 von 39

1

Fülle die Lücken, um den Text zu vervollständigen.

Fill in the blanks below to describe how proteins are targeted to the mitochondria.
1. The is found at the terminal of the protein.
2. Cytosolic binds to the protein using energy from to prevent the protein from .
3. The binds to an on the outer membrane.
4. The targets the protein through . Meanwhile, is cleaved from the protein using hydrolysis.
5. The protein enters the matrix via import pores and TIM23/.
6. The protein binds to matrix to prevent it from .
7. enzyme cleaves the from the protein.
8. The protein can now begin .

Erklärung

Frage 22 von 39

1

When does the targeting of proteins to the nucleus occur?

Wähle eine der folgenden:

  • After folding

  • After translation before folding

Erklärung

Frage 23 von 39

1

What property does the Nuclear Localisation Signal have that allows it to target?

Wähle eine der folgenden:

  • Basic

  • Acidic

  • Polar

  • Hydrophobic

Erklärung

Frage 24 von 39

1

What protein does the nuclear localisation sequence bind to?

Wähle eine der folgenden:

  • Importin

  • Ran

  • RanGEF

  • SNARE

Erklärung

Frage 25 von 39

1

Ran binds to GTP in the cytosol.

Wähle eins der folgenden:

  • WAHR
  • FALSCH

Erklärung

Frage 26 von 39

1

What converts RanGDP to RanGTP in the cytosol?

Wähle eine der folgenden:

  • Ran guanine nucleotide exchange factor (RanGEF)

  • MAPK

  • MAPKK

  • ATP

Erklärung

Frage 27 von 39

1

What happens to RanGTP in the nucleus?

Wähle eine der folgenden:

  • Binds to importin

  • Binds to Nuclear Localisation Sequence

  • Activated by MAPK

  • Destroyed by RanGEF

Erklärung

Frage 28 von 39

1

Importin and RanGTP are recycled by their exit from the nucleus after targeting takes place.

Wähle eins der folgenden:

  • WAHR
  • FALSCH

Erklärung

Frage 29 von 39

1

What does cytosolic Ran GTPase activated protein do?

Wähle eine der folgenden:

  • Stimulates hydrolysis of RanGTP to RanGDP

  • Stimulates formation of RanGTP from RanGDP

  • Activates binding of importin to RanGTP

  • Activates cleavage of importin from Nuclear Localisation Sequence

Erklärung

Frage 30 von 39

1

What triggers the release of importin from Ran?

Wähle eine der folgenden:

  • Hydrolysis of RanGTP to RanGDP

  • Change in pH of the cytosol

  • Formation of RanGTP from RanGDP

  • Action of RanGEF

Erklärung

Frage 31 von 39

1

Fülle die Lücken, um den Text zu vervollständigen.

Fill in the blanks below to describe the targeting of proteins to the nucleus.
1. The on the protein binds to in the cytosol.
2. Ran binds to in the cytosol.
3. Both of these complexes enter the nucleus via .
4. In the nucleus, Ran (RanGEF) converts RanGDP to .
5. binds to . This disrupts 's ability to bind to the . The protein is released.
6. Importin and Ran exit the nucleus via a nuclear pore.
7. Cytosolic Ran (Ran GAP) stimulates Ran to hydrolyse to .
8. allows the release of .

Erklärung

Frage 32 von 39

1

What are lysosomal proteins tagged with in the Golgi apparatus?

Wähle eine der folgenden:

  • Mannose-6-phosphate

  • Glucokinase

  • Phosphate

  • Importin

Erklärung

Frage 33 von 39

1

Mannose-6-phosphate and lysosomal proteins are targeted to transport vesicles in the Golgi via what?

Wähle eine der folgenden:

  • M6P receptors

  • Importin

  • TOM40

  • SNAREs

Erklärung

Frage 34 von 39

1

What do transport vesicles from the Golgi containing lysosomal proteins fuse with?

Wähle eine der folgenden:

  • Early endosome

  • Cell membrane

  • Cis face of Golgi apparatus

  • Endoplasmic reticulum

Erklärung

Frage 35 von 39

1

ATP synthase continuously pumps H+ into the endosome containing lysosomal proteins. What does this cause?

Wähle eine der folgenden:

  • Dissociation from M6P receptor and a phosphate to form mature hydrolase

  • Dissociation from M6P receptor only

  • Dissociation from a phosphate to form mature hydrolase

  • Cleavage of the lysosomal protein into two products

Erklärung

Frage 36 von 39

1

M6P receptors used in lysosomal proteins are targeted back to the Golgi.

Wähle eins der folgenden:

  • WAHR
  • FALSCH

Erklärung

Frage 37 von 39

1

Fülle die Lücken, um den Text zu vervollständigen.

Fill in the blanks below to describe how proteins are targeted to the lysosomes.
1. Lysosomal proteins are tagged with in the .
2. This complex binds to receptors and is packaged into .
3. bud off the face of the Golgi apparatus and travel to the early with which they fuse.
4. continuously pumps ions into the enzyme, reducing the .
5. The low causes the to be dissociated from the complex as well as to form a mature hydrolase protein.
6. The receptors are recycled back to the via transport .

Erklärung

Frage 38 von 39

1

What disease is caused by a mutation in the enzyme that phosphorylates mannose?

Wähle eine der folgenden:

  • Inclusion-cell disease

  • Hartnup disease

  • Wernicke-Korsakoff syndrome

  • Cystic fibrosis

Erklärung

Frage 39 von 39

1

Fülle die Lücken, um den Text zu vervollständigen.

In disease, the enzyme that phosphorylates is mutated. This means that lysosomal proteins aren't tagged with so are not targeted to the . The therefore lose their function and accumulates within the cells. This causes developmental defects and often death before the age of 10 due to heart failure/pneumonia.

Erklärung

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