Study Guide 14 - Winter 2015

Frage 1 von 31

1

What would be the likely cellular effects of a large deletion in the gene encoding the polymerase responsible for adding 3′ poly(A) tails to eukaryotic mRNAs?

Wähle eine der folgenden:

Inactivation of the polymerase would lead to incomplete pre-mRNA processing, including 3′ end formation, splicing, editing, and transport—and would certainly be lethal.
Activation of the polymerase would lead to incomplete pre-mRNA processing, including 3′ end formation, splicing, editing, and transport—and would certainly be lethal.

Erklärung

Frage 2 von 31

1

Compare and contrast splicing mechanisms used by spliceosomes, group I introns, and group II introns, with respect to the nucleophiles, proteins, or nucleic acids involved and how the specificity of splice sites is achieved.

Wähle eine der folgenden:

Group I and group II introns are generally self-splicing, with only the RNA backbone of the intron required for the reaction in vitro. The nucleophile in the first step, cleavage of the 5′ splice site, is a guanine nucleotide or nucleoside for group I introns, and the 2′-OH of an internal A residue for group II introns. The second step uses the liberated 3′-OH at the 5′ splice site as nucleophile to attack the phosphodiester bond at the 3′ splice site, joining the exons. Site specificity is aided by guide sequences that are part of the intron structures. Introns removed with spliceosomes follow a mechanism similar to group II introns, but also rely on the spliceosome ribonucleoprotein complex to catalyze their excision.
None

Erklärung

Frage 3 von 31

1

Self-splicing introns do not require an energy source, such as ATP or GTP, to catalyze splicing. How does self-splicing proceed with a reasonable yield of products?

Wähle eine der folgenden:

Self-splicing catalyzes phosphodiester exchange reactions with no net loss or gain of energy. Bonds are broken and re-formed with different nucleotides, and there is no change in the number of phosphodiester bonds, just in the covalent bonding partners. Thus, there is no net change in free energy from reactants to products.
none

Erklärung

Frage 4 von 31

1

Which of the following nuclear pre-mRNA nucleotide sequences potentially contains an intron?

Wähle eine der folgenden:

5'--UGACCAUGGCGCUAACACUGCCAAUUGGCCAAUACUGACCUGAUAGCAUCAGCCAA--3'
5'--UAGUCUCAUCUGUCCAUUGACUUCGAAACUGAAUCGUAACUCCUACGUCUAUGGA--3'
5'--UAGCUGUUUGUCAUGACUGACUGGUCACUAUCGUACUAACCUGUCAUGCAAUGUC--3'
5'--UAGCAGUUCUGUCGCCUCGUGGUGCUGGCUGGCCCUUCGUCGCUCGGGCUUAGCUA--3'
5'--UAGGUUCGCAUUGACGUACUUCUGAGACUACUAACUACUAACGCAUCGAGUCUCAA--3'

Erklärung

Frage 5 von 31

1

One of the five pre-mRNAs shown above is a likely candidate for splicing out an intron sequence. What mRNA nucleotide sequence would one expect to result from this splicing event?

Wähle eine der folgenden:

5'--UAGUCUCAA--3'; the putative intron from the 5' GU through the 3' AG has been removed.
none

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Frage 6 von 31

1

Eukaryotic messenger RNA and hnRNA both have ___________ at their 3'-ends.

Wähle eine der folgenden:

m7 G caps
poly(A) tails
hairpin structures
covalently bound proteins

Erklärung

Frage 7 von 31

1

Eukaryotic messenger RNA molecules have _______________ at their 5' ends.

Wähle eine der folgenden:

m7 G caps
poly(A) tails
hairpin structures
covalently bound proteins

Erklärung

Frage 8 von 31

1

Eukaryotes use a variety of pathways to form 5'-m7 G caps.

Wähle eins der folgenden:

WAHR
FALSCH

Erklärung

Frage 9 von 31

1

The carboxy terminal domain of the largest subunit of RNA polymerase II must be _______________ on Ser-5 to target a transcript for capping.

Wähle eine der folgenden:

methylated
phosphorylated
glycosylated
dephosphorylated

Erklärung

Frage 10 von 31

1

Amino acid-coding regions within eukaryotic genes may be interrupted by ___________ regions.

Wähle eine der folgenden:

noncoding
long terminal repeat
split gene
enhancer

Erklärung

Frage 11 von 31

1

Exons tend to be conserved during evolution, whereas introns usually are not as tightly conserved.

Wähle eins der folgenden:

WAHR
FALSCH

Erklärung

Frage 12 von 31

1

Two splicing intermediates resemble ______________.

Wähle eine der folgenden:

hairpins
projections
lariats
helices

Erklärung

Frage 13 von 31

1

_______________ permits a cell to recode genetic information in a systematic and regulated fashion.

Wähle eine der folgenden:

Replication
Transcription
RNA editing
DNA repair

Erklärung

Frage 14 von 31

1

Pre-mRNA requires specific sequences for precise __________ to occur.

Wähle eine der folgenden:

skipping
splicing
tagging
replication

Erklärung

Frage 15 von 31

1

A single _____________ can be processed to produce two or more different mRNA molecules.

Wähle eine der folgenden:

tRNA
pre-mRNA
snRNP
ribosome

Erklärung

Frage 16 von 31

1

_________________ assemble to form a spliceosome, the splicing machine that excises introns.

Wähle eine der folgenden:

rRNAs
snRNPs
tRNAs
Micro RNAs

Erklärung

Frage 17 von 31

1

What is the key component of the catalytic site of the spliceosome?

Wähle eine der folgenden:

DNA
protein
ribisomes
RNA

Erklärung

Frage 18 von 31

1

Transcription termination takes place downstream from the _______________

Wähle eine der folgenden:

5'-m7 G caps
poly(A) site
promoter region
enhancer sequences

Erklärung

Frage 19 von 31

1

_________________ molecules can silence gene expression.

Wähle eine der folgenden:

Knockout
Short RNA
Spliceosome
Protease

Erklärung

Frage 20 von 31

1

The ______________ pathway blocks mRNA translation or causes mRNA degradation.

Wähle eine der folgenden:

spliceosome
miRNA
ribozyme
snRNP

Erklärung

Frage 21 von 31

1

Splicing begins as a cotranscriptional process and continues as a posttranscriptional process.

Wähle eins der folgenden:

WAHR
FALSCH

Erklärung

Frage 22 von 31

1

One form of severe thalassemia is known to be the result of a mutation that causes abnormal RNA splicing because of a five nucleotide deletion that disrupts the first splice donor signal of the beta globin pre-mRNA. All thalassemias result in a reduced rate of synthesis of one of the globin chains (either alpha or beta globin) that make up hemoglobin. Which of the following techniques would best show the presence of the abnormal splicing in a suspected carrier of the disease?

Wähle eine der folgenden:

Analysis to detect altered mobility of beta globin protein by electrophoresis
Analysis to detect decreased oxygen binding by red blood cells
Analysis to detect altered size of beta globin protein
Analysis to detect altered DNA sequence of promoter regions
Analysis to detect altered size of beta globin mRNA

Erklärung

Frage 23 von 31

1

Figure 1 represents regions around a gene encoding a mRNA. Exon I of the mRNA contains the translation initiation AUG codon. Exon IV contains the UGA termination codon. The letters A through E above the diagram refer to areas of the gene and its transcript located directly below them.

Which of the lettered regions of the primary transcript is removed by a spliceosome?

Wähle eine der folgenden:

A
B
C
D
E

Erklärung

Frage 24 von 31

1

Figure 1 represents regions around a gene encoding a mRNA. Exon I of the mRNA contains the translation initiation AUG codon. Exon IV contains the UGA termination codon. The letters A through E above the diagram refer to areas of the gene and its transcript located directly below them.

What region of the DNA contains the TATA box?

Wähle eine der folgenden:

A
B
C
D
E

Erklärung

Frage 25 von 31

1

Figure 1 represents regions around a gene encoding a mRNA. Exon I of the mRNA contains the translation initiation AUG codon. Exon IV contains the UGA termination codon. The letters A through E above the diagram refer to areas of the gene and its transcript located directly below them.

Where is the poly (A) tail added to the transcript?

Wähle eine der folgenden:

A
B
C
D
E

Erklärung

Frage 26 von 31

1

Figure 1 represents regions around a gene encoding a mRNA. Exon I of the mRNA contains the translation initiation AUG codon. Exon IV contains the UGA termination codon. The letters A through E above the diagram refer to areas of the gene and its transcript located directly below them.

Where is the 5' cap added to the transcript?

Wähle eine der folgenden:

A
B
C
D
E

Erklärung

Frage 27 von 31

1

If you used radioactively labeled mRNA produced from the galactose operon (encodes for the galE, galT, and galK genes) as a probe in a southern blot experiment, the mRNA probe would NOT hybridize to which one of following DNA sequences?

Wähle eine der folgenden:

The galE gene
The galT gene
The galK gene
The galactose operon P1 or P2 promoters
A bogus question. The mRNA probe would bind to each of the DNA sequences listed above

Erklärung

Frage 28 von 31

1

RNA interference (RNAi) is regulatory mechanism at the

Wähle eine der folgenden:

transcriptional level.
post-transcriptional level.
translational level.
post-translational level.
genomic organization level.

Erklärung

Frage 29 von 31

1

The trigger for RNA interference (RNAi) is

Wähle eine der folgenden:

double stranded DNA
single stranded RNA
double stranded RNA
double stranded RNA to which a single strand RNA is base paired
single stranded RNA with a nick

Erklärung

Frage 30 von 31

1

You want to clone an insert containing a gene-of-interest into a plasmid vector. Which of the following is TRUE?

Wähle eine der folgenden:

Both the insert and plasmid must be cut with the same restriction enzyme
Complementary sticky ends from different DNA molecules cannot base pair
After ligation, the next step is transposition into a bacterial host.
A replication origin is required in both the plasmid and the insert.
More than one of the above statements (A-D) is true

Erklärung

Frage 31 von 31

1

For the experiment below, cDNA from kidney was labeled green, and cDNA from lung was labeled red. The probes from the two samples were mixed together and hybridized to a microarray, giving the results below. The numbers represent fluorescent intensity for genes AF. Assume that the red and green fluorescent labels give equivalent signal.

kidney lung
green red
gene A 2,000 1,900
gene B 3,000 1,000
gene C 2,000 5,000
gene D 1,500 5,000
gene E 800 1,000
gene F 9,000 10,000

Which gene(s) would you select for study if you were interested in genes required specifically for kidney function?

Wähle eine der folgenden:

Genes F and C
Genes F, A, and E
Gene D
Gene C
Gene B

	Erstellt von John Bench vor mehr als 9 Jahre

Quiz am Study Guide 14 - Winter 2015, erstellt von John Bench am 22/03/2015.

Study Guide 14 - Winter 2015

Frage 1 von 31

What would be the likely cellular effects of a large deletion in the gene encoding the polymerase responsible for adding 3′ poly(A) tails to eukaryotic mRNAs?

Wähle eine der folgenden:

Erklärung

Frage 2 von 31

Compare and contrast splicing mechanisms used by spliceosomes, group I introns, and group II introns, with respect to the nucleophiles, proteins, or nucleic acids involved and how the specificity of splice sites is achieved.

Wähle eine der folgenden:

Erklärung

Frage 3 von 31

Self-splicing introns do not require an energy source, such as ATP or GTP, to catalyze splicing. How does self-splicing proceed with a reasonable yield of products?

Wähle eine der folgenden:

Erklärung

Frage 4 von 31

Which of the following nuclear pre-mRNA nucleotide sequences potentially contains an intron?

Wähle eine der folgenden:

Erklärung

Frage 5 von 31

One of the five pre-mRNAs shown above is a likely candidate for splicing out an intron sequence. What mRNA nucleotide sequence would one expect to result from this splicing event?

Wähle eine der folgenden:

Erklärung

Frage 6 von 31

Eukaryotic messenger RNA and hnRNA both have ___________ at their 3'-ends.

Wähle eine der folgenden:

Erklärung

Frage 7 von 31

Eukaryotic messenger RNA molecules have _______________ at their 5' ends.

Wähle eine der folgenden:

Erklärung

Frage 8 von 31

Eukaryotes use a variety of pathways to form 5'-m7 G caps.

Wähle eins der folgenden:

Erklärung

Frage 9 von 31

The carboxy terminal domain of the largest subunit of RNA polymerase II must be _______________ on Ser-5 to target a transcript for capping.

Wähle eine der folgenden:

Erklärung

Frage 10 von 31

Amino acid-coding regions within eukaryotic genes may be interrupted by ___________ regions.

Wähle eine der folgenden:

Erklärung

Frage 11 von 31

Exons tend to be conserved during evolution, whereas introns usually are not as tightly conserved.

Wähle eins der folgenden:

Erklärung

Frage 12 von 31

Two splicing intermediates resemble ______________.

Wähle eine der folgenden:

Erklärung

Frage 13 von 31

_______________ permits a cell to recode genetic information in a systematic and regulated fashion.

Wähle eine der folgenden:

Erklärung

Frage 14 von 31

Pre-mRNA requires specific sequences for precise __________ to occur.

Wähle eine der folgenden:

Erklärung

Frage 15 von 31

A single _____________ can be processed to produce two or more different mRNA molecules.

Wähle eine der folgenden:

Erklärung

Frage 16 von 31

_________________ assemble to form a spliceosome, the splicing machine that excises introns.

Wähle eine der folgenden:

Erklärung

Frage 17 von 31

What is the key component of the catalytic site of the spliceosome?

Wähle eine der folgenden:

Erklärung

Frage 18 von 31

Transcription termination takes place downstream from the _______________

Wähle eine der folgenden:

Erklärung

Frage 19 von 31

_________________ molecules can silence gene expression.

Wähle eine der folgenden:

Erklärung

Frage 20 von 31

The ______________ pathway blocks mRNA translation or causes mRNA degradation.