Process used in an expression vector to increase yield of recombinant protein synthesis ?
Translation initiation
Promoter induction
Transcription terminators
Multiple cloning sites
Rothera’s test is utilised for detection of?
Glucose
Proteins
Urea
Ketone bodies
Decreased energy production in thiamine deficiency is due to?
It is a cofactor for oxidative reduction
It is required for transamination reactions
It is a coenzyme for alpha ketoglutarate dehydrogenase and pyruvate dehydrogenase in citric acid cycle
It is a coenzyme for transketolase in hexose monophosphate shunt
Which of the following enzymes is responsible for the transfer of amino groups from an amino acid to an alpha keto acid?
Transaminase
Transketolase
Deaminase
Lyase
Site of urea cycle is?
Liver
Kidney
GIT
Lungs
Which of the following is not a protein misfolding disorder?
Tuberculosis
Creutzfeldt Jakob disease
Alzheimer’s disease
Cystic fibrosis
Sphingomyelinase deficiency is characteristic of ?
Fabry disease
Krabbe’s disease
Tay Sachs disease
Niemann Pick disease
Xeroderma pigmentosum is produced as a result of a defect in :
DNA polymerase III
DNA exonuclease
DNA ligase
none
A genetic disorder renders fructose 1,6 — biphosphatase in liver less sensitive to regulation by fructose 2,6 — bi-phosphate. All of the following metabolic changes are observed in this disorder except:
Level of fructose 1,6—biphosphate is higher than normal
Level of fructose 1,6 — biphosphate is lower than normal
Less pyruvate is formed
Less ATP is generated
Alpha helix and Beta pleated sheet are examples of:
Primary structure
Secondary structure
Tertiary structure
Quaternary structure
