Step 1 of Prosection sequence includes:
External Examination
Body weight/condition score
External Abnormalities
Initial dissection
Step 2 of Prosection sequence (after external exam) includes:
Axillary/ inguinal incisions
Joining incisions laterally & ventrally reflecting skin
Reflecting tongue
Step 3 of Prosection (after incision of axilliary/inguinal regions) includes:
Examination of peripheral lymph nodes
Reflection of tongue
Removal of adrenals
Step 4 of Prosection (After examination of peripheral lymph nodes) includes:
Incise soft palate & Hyoid
Dissect oesophagus & trachea as one
Dissect oesophagus & trachea separately
What are the peripheral lymph nodes examined in step 3 ?
Submandibular, auxillary, prescapular
Submandibular, prescapular, popliteal
Prescapular, submandibular, inguinal
Step 5 of prosection (after reflection of the tongue) includes:
Cut abdominal muscles around costal arch
Incise abdominal wall down linea alba (Xiphoid to pubis)
Central midline incision
Open abdomen
Step 6 of prosection (after opening abdomen) includes:
Examine lymph nodes
Open thorax
Cut ribs from costal arch to thoracic inlet bilaterally
Remove abdominal organs for examination
Step 7 of prosection ( after opening thorax) includes:
Remove thoracic viscera
Separate trachea and oesophagus
Transect aorta and venacava
removal of trachea, heart and lungs leaving oesophagus in situ
Step 8 of Prosection ( after removal of thoracic viscera) includes:
Removal of abdominal viscera
Examination of abdominal viscera
Ligate and transect descending colon
Cut root of mesentery and remove intestines, stomach, liver and spleen as one unit
Step 9 or prosection (After removal of abdominal viscera) includes:
Removal of thyroid gland
Removal of kidneys
Step 10 of prosection (After removal of adrenal glands) includes:
Removal of ventral pelvis to expose pelvic viscera
Freeing kidney and ureters
Dissection of major limb joints
Removal of urino-genital unit with encircling incision around anus
Step 11 of prosection (after removal of ventral pelvis) includes:
Dissection of kidneys
Dissection of lymph nodes
Step 12 of prosection ( after dissection of major limb joints) includes:
Removal of head at alanto-occipital joint
saw off calvarium to expose brain
Incise meninges and remove brain (leave pituitary in situ)
Incise meninges and remove brain and pituitary
Step 13 of prosection (after removal of brain) includes:
Examination of individual organ systems
Examination of special sense organs
Examination of brain
Step 14 of prosection (After examination/removal of special sense organs) includes:
Examination of urinary system
Examination of lymph nodes
Histopathology sampling should include:
Freeze tissue at -20 degrees C
Sensible sizes of tissue
Fixing in 10% Formalin
10X volume of Formalin/ 1 volume of tisse
Toxicology of samples involves:
Freezing at 20 degrees C unfixed
Fixing in 10% formalin
Refrigerating at 4 degrees C
Cytology sampling involves:
Unfixed fluids sent to a lab ASAP
Smears are air dried
Freezing samples at - 20 degrees C
