Which naturally occurring antibody(ies) is(are) found in the serum of a group A Rh negative patient

Holes are noted in a cryostat section of muscle. Which of the following is the most likely cause?

Which of the following is true regarding H&E staining using an automated stainer?

Which of the following is true of quality control materials for internal QC?

During a routine quality control check, a Kirby-Bauer disc susceptibility test is performed and the resulting zones of inhibition are too large. Which of the following is a possible cause of this result?

Which antibody could be produced by an R1r individual transfused with R2R2 blood?

A good screening test for alcohol ingestion employs comparing the calculated osmolality with what other measurement?

When performing a D typing on a patient, a technologist observes that the Rh control is positive. As a result, which of the following would he perform next?

When entering blood gas results manually into the LIS what should be done if a critical value warning appears when a pH value of 7.14 is entered?

Gram positive cocci in chains which grow as small translucent colonies surrounded by a clear zone of hemolysis were isolated from a throat swab. These colonies were found to be catalase negative and resistant to a 0.04 unit bacitracin disc. Which of the following tests would be most useful in identifying this organism?

What should be done with patient results that are outside reference intervals but are within the percent differences programmed into the delta check?

What statement about the dry reagent strip blood test is true?

Both hemoglobin and myoglobin will react with the H2O2 on the pad, which gives a positive result. The colour of the urine (brown vs red) and microscopic examination (red cells) can be used to differentiate haemoglobin from myoglobin. Oxidizing reagents such as bleach can also cause a false positive reaction for blood test, however, salicylate is reducing reagent. Oxidase is used to test for urine glucose.

Which of the following antibodies would not show any reactivity when tested against a panel of reagent red blood cells which had been treated with ficin?

What does staining leukemic cells for myeloperoxidase help to distinguish?

A suspect Enterobacteriaceae isoalte was inoculated to a TSI and decarboxylase test. The TSI showed K/A with gas. The decarboxylase showed:

base: purple, turbid
lysine: purple, turbid
ornithine: purple, turbid

These results indicate:

Given the following patient test results, what is the creatinine clearance?
serum creatinine 100 μmol/L
urine creatinine 8.64 mmol/L
24 hour urine volume 2000 mL
BSA 1.73m^2

What happens to hemoglobin's affinity for oxygen when hemoglobin binds 2,3-DPG?

The Philadelphia chromosome is predominately associated with which leukemia?

A technologist is performing a nitrate reduction test. When dimethyl-α-naphthylamine and sulfanilic acid were added, the medium did not change colour. What should the technologist do?

The alcian blue staining reactions of colonic epithelial goblet cells on serial sections from the same tissue block show the following staining results:

Alcian Blue @ pH 1.0 1+ (stain intensity)
Alcian Blue @ pH 2.5 3+
Alcian Blue @ pH 4.5 3+

If QC was performed on a batch of Hugh-Leifson O-F (oxidative-fermentative) media, which of the following results would correspond with acceptable QC?

A 26 year old female out-patient presents to her physician with symptoms of uncomplicated cystitis. A culture of her urine grows gram positive cocci. What organism would these cocci likely represent?

What should a technologist do if she obtains low results for all analytes in a specimen?

What may cause wrinkles in sections during flotation?

How many units of packed red cells would be required to raise the hemoglobin of an average sized patient from 80 g/L to 110 g/L?

What does the D-dimer lab test detect?

A control slide is stained with H&E using an automated tissue stainer. Upon microscopic assessment following staining in Harris hematoxylin, the nuclei appear blue-purple with an obvious chromatin pattern. What would be the appropriate action at this stage?

A midstream urine is received in the laboratory from a recently discharged inpatient. The requisition indicates the date of collection as yesterday making the specimen 24.5 hours old but the specimen feels warm to the touch. What should the technologist do?

What would processing tissues through alcohol stations that are contaminated with water initially result in?

Every piece of equipment in the laboratory has a log which is used to document QC values during start-up. How can this log be used?

Which of the following is recommended for the detection of penicillin resistance in Streptococcus pneumoniae?

A cloudy cerebrospinal fluid has an increased leukocyte count and a decreased glucose concentration. What is the most probable type of meningitis in a patient with these CSF results?

Which of the following contains only ferric iron?

An immunocompromised 58-year-old female chemotherapy patient received two units of packed RBC. The patient died 3 days later, and the report from the autopsy revealed that her death was due to septic shock. The blood bags were cultured and the following results were noted. Growth of gram-negative rods on both the BAP and MAC

Lactose neg
Sucrose pos
Citrate neg
Indole neg
VP neg
H2S neg
Urea pos
motility @ 22°C pos
motility @ 37°C neg

A grossly lipemic specimen needs to be tested for potassium using a direct ISE method. How should the technologist proceed?

How should a potassium value of 15 mmol/L be interpreted?

What is an appropriate disposal method for ammoniacal silver solutions?

What should be done when a laboratory reporting error is corrected?

Which of the following mothers is a candidate for Rh immune globulin?

A standard with a transmission value of 50% and a concentration of 100 g/L is used to estimate an unknown specimen with a transmission value of 75%. What is the concentration of the unknown specimen in g/L?

Which of the following methods is the safest and most appropriate for disinfecting a cryostat where adequate ventilation is not available?

A single tube of cerebrospinal fluid is received in the laboratory and the following tests are requested: total protein, albumin, IgG quantitation, microbial culture, Gram stain, leukocyte count and differential cell count. Where should the sample tube be sent first?

A patient is positive for anti-Fya, anti-Jkb and anti-K. How many units of blood will have to be screened to find two compatible units, given the following antigen frequencies?

Fya positive = 60%
Jkb positive = 70%
K positive = 10%

Two levels of QC material and the Westgard 1 2S rule as a warning rule to check the other quality control rules are being used by a technologist. On the same run, one of your QC values is +2.1SD while the other QC value is -2.2SD. What action should be taken on the basis of these QC results?

Which of the following would lead to an inpatient total serum calcium result of less than 1 mmol/L?

What is the calculated serum osmolality in mOsm/kg given the following serum results?
Sodium 145 mmol/L
Potassium 4.2 mmol/L
Chloride 104 mmol/L
Bicarbonate 26 mmol/L
Urea 5 mmol/L
Glucose 10.3 mmol/L

Which of the following assays has the poorest precision?

Analyte Mean (mmol/L) Standard Deviation
Calcium 2.5 0.3
Potassium 4.0 0.4
Sodium 140 4.0
Chloride 100 2.5

Blood from a newborn has low thyroxine (T4) and elevated TSH compared to reference ranges for that age. These results are most consistent with

A pediatric renal transplant patient had a suprapubic urine collection performed. In the lab, a technologist plated the specimen following protocol with a 0.1 mL calibrated loop. At 18 hours there are 8 colonies of a coliform growing on the plate. What colony count would be reported?

Which of the following is/are true about a cryostat

A technologist did the following to a frozen section of liver. the section was taken through to 70% alcohol, treated with saturated Oil red O in 70% alcohol, rinsed with 70% alcohol, then the nuclei were stained with Harris hematoxylin. The technologist then dehydrated, cleared and mounted the sections in Permount. What results should she see under microscopic observation if unsaturated fats were present in the frozen section.

You have been asked to freeze a muscle for Muscle Histochemistry. You place isopentane into liquid nitrogen until it has begun to freeze you then place the muscle into the isopentane. The result of your actions are which of the following?

When testing your patient sample, you find all 3 screen cells positive at IAT phase. When an antibody panel is done, all cells except one are positive. The patient auto control is negative. Which of the following is the most likely explanation for this reaction pattern?

Chem 7, 9, 12, 17, 18, 24, 29, 34, 36, 37, 40, 43, 46, 47, 50
Micro 5, 10, 16, 20, 22, 23, 32, 33, 35, 43, 51
Hem 13, 15, 19, 27, 43
Transfusion 1, 6, 8, 14, 26, 40, 44, 55
AP 2, 3, 21, 25, 28, 30, 38, 42, 52, 53, 54
QC 3, 4, 5, 22, 28, 31, 45, 48, 49
Calculation 13, 17, 26, 40, 44, 47, 48, 49, 51
Situational/Troubleshoot 2, 8, 9, 11, 24, 25, 29, 37, 39, 43
Tricky 1, 16, 17, 28, 30, 36, 40, 49, 51, 52, 53

This is a categorization of all the questions in this quiz. Hopefully, this will give you an idea of which area you may need to focus on (if any).