Reason for Cellular Transporters

Role of Transport Proteins

Giant Squid Axon

Anteriograde transport

Retrograde transport

ATP

Protein that makes up microtubules

Distance between each motor protein binding site

Size of microtubules

+ve end of microtubule

-ve end of microtubule

Microtubule ATP requirement

No of Kinesin types in cell

Kinesin Superfamily

Direction of Kinesin movement

Structure of Kinesin 1- Chains

Design of Kinesin 1 - Cargo binding

Design of Kinesin 1 - Stalk

Design of Kinesin 1 - Microtubule binding sites

Design of Kinesin 1 - Neck Linkers

Role of Neck linker

Role of coiled coil stalk

Sliding assay

Rate of Kinesin movement

Processive movement

Monomeric heavy chain kinesin mutants

Potential Models for movement of Kinesin

Optical Trap

Inchworm Model

Hand Over Hand model

Determining Kinesin Movement Method

Precision of Fluorophore Method

Role of Switch's I & II

Movement of Switch II

ATP Binds to sensor

ATP binding & sensor relationship

Relay Helix

Conformational changes of ATP binding

12 amino acid loop