Which neurotransmitter was used to stimulate the muscle tissue?

What type of muscle tissue was used?

What type of relationship exists between the muscle activity and the concentration of the drug added?

Which piece of technical equipment was the force transducer attached to?

Which piece of software was used in the lab?

What units is the raw output of the force transducer measured in? What did you want the units to be?

How heavy was the weight used to calibrate the force transducer?

What solution was the tissue kept in? Why was this?

What were the relative proportions of O2 and CO2 provided to the tissue? At what volume was this provided?

If 1ml of 1 x 10^-5M acetylcholine solution is added to 9ml of physiological solution, what is the final tissue bath concentration of drug that the tissue experiences?

The bladder detrusor muscle took longer to plateau (maximal force exerted by maximal contraction at that concentration of acetylcholine) than the rat intestinal smooth muscle in the last organ bath. How is this related to its function?

What would you expect to happen to the response (in grams) as the concentration of acetylcholine increased?

Given that the atropine and acetylcholine powder were not in contact with students, what was the biggest risk in this lab?