OCR A2 Biology: Gene Technologies

What are gene technologies?

What is PCR be used for?

Outline the first step of PCR

What is contained in this reaction mixture?

What are primers?

What is DNA Polymerase?

What is the second stage of PCR?

What is the third stage of PCR?

What does the DNA Polymerase do?

What is produced in the first cycle of PCR?

What do restriction enzymes do?

What are palindromic sequences?

How do restriction enzymes work?

How can you isolate a piece of DNA using restriction enzymes?

What does hydrolysis of DNA sometimes leave?

What are sticky ends?

What can sticky ends be used for?

What is gel electrophoresis?

Why is a fluorescent tag added to the DNA fragments in gel electrophoresis?

What is the DNA placed in during gel electrophoresis?

Why do DNA move to the positive electrode in gel electrophoresis?

Why does this technique separate the fragments by size?

What are DNA probes used for?

What are DNA probes?

What are the most common labels used in DNA probing?

Outline the process of DNA probes that are labelled with a UV label.

What is genetic engineering?

What is recombinant DNA?