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costs associated with bringing recombinant protein to market
what influences the choice of organism?
plasmids can carry up to:
cosmids carry up to
general organism issues (recombinant protein production)
advantages of e coli for recombinant protein production
disadvantages of e coli for recombinant protein production
somatostatin (first reombinant protein made in e coli- anti growth hormone)
recombinant insulin (2 ways)
if your protein is active as a single unprocessed polypeptide...
If the activity of your protein is based on it’s complexity ....
7 post translational modifications of proteins
Pros of yeast and fungi
cons of yeast and fungi
pros of insect cells
cons of insect cells
plant recombinants
mammalian cells for recombs
glycosylation problems in higher eukaryotes
Gal alpha 1-3 Gal
rationale for recombinant protein production
are recombinants really safer?
all recombinants must be tested before human use
what are the in vivo animal tests looking at?
requirements for recombinant protien production
Waht do we need to make a recombinant protien?
recombinant protiens
where may recombinant protiens be produced?