Microbiology Lab Practical

Frage 1 von 127

1

What method of staining was used to create this slide?

Wähle eine der folgenden:

Acid-fast Stain
Endospore Stain
Gram Stain
Negative Stain

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Frage 2 von 127

1

What kind of staining process was used to make this slide?

Wähle eine der folgenden:

Negative Stain
Endospore Stain
Gram Stain
Acid-fast Stain

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Frage 3 von 127

1

What kind of microbes are present on this slide?

Wähle eine der folgenden:

Gram (-)
Endospores
Nocardia
Mycobacterium

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Frage 4 von 127

1

Where should your backpacks and purses be stored during the lab period?

Wähle eine der folgenden:

On the shelf under the lab bench.
On the floor next to your seat.
On the lab bench.

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Frage 5 von 127

1

What items should you wear to protect yourself in the lab.

Wähle eine oder mehr der folgenden:

Lab coat
Safety Glasses
Gloves
Closed toe shoes
Hair net
Shoe covers

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Frage 6 von 127

1

When do you disinfect your lab table and where do you discard the used paper towels?

Wähle eine oder mehr der folgenden:

Disinfect on the way into the lab.
Disinfect on the way out of the lab.
Paper towels go in the regular trash.
Paper towels go in the autoclave trash.
Disinfect between each step of the lab process.

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Frage 7 von 127

1

You are dying of thirst (hunger, for smoke, chapped lips, bad breath) during the lab period. What do you do to alleviate your problem?

Wähle eine der folgenden:

Leave
Disinfect your lab bench, take care of your need and then disinfect your lab bench, again.
Go to the corner of the lab room and take care of your need.

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Frage 8 von 127

1

You notice your lab partner has caught the lab manual on fire. What should you do?

Wähle eine oder mehr der folgenden:

Alert the professor
Get the fire extinguisher near the front door
Get the fire extinguisher from the back of the room
Get deionized water from the back of the room and pour it on the fire

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Frage 9 von 127

1

Your lab partner has managed to spill microorganisms all over the lab table. What should be done to clean up the spill?

Wähle eine oder mehr der folgenden:

Alert the professor
Spray disinfectant on the spill
Spray disinfectant on a paper towel and place on the spill
Keep spraying disinfectant on the paper towel
Wait 10 minutes
Wait 15 minutes
Dispose in autoclave trash
Dispose in regular trash

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Frage 10 von 127

1

A student broke an empty glass test tube. What should be done to clean up the glass and where should the pieces of glass be disposed of?

Wähle eine oder mehr der folgenden:

Clean with dust pan and broom next to the fridge.
Clean with dust pan and broom near the front door.
Dispose of in the broken glass disposal box.
Dispose of in the autoclave trash.
Dispose of in the regular trash.

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Frage 11 von 127

1

The instructor tripped on a backpack on the floor knocking her safety goggles off and splashing HCl in her eyes. What should she do?

Wähle eine oder mehr der folgenden:

Use the eyewash located near the front door.
Use the eyewash located at the back of the room.
Use the eyewash next to the fridge.
Use the eyewash near back of the room.

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Frage 12 von 127

1

After surviving the lab period you remove your gloves by turning them inside out and then where do you toss them?

Wähle eine oder mehr der folgenden:

In the autoclave trash
In the regular trash
In the used glove disposal bin

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Frage 13 von 127

1

Choose the two major types of microscopes that have been developed and the difference between them

Wähle eine oder mehr der folgenden:

Compound and Electron.
Compound uses light and Electron uses electrons.
Compound and Bright Field.
Compound uses electrons and Bright Field uses light.
Compound uses light and Bright Field uses electrons.

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Frage 14 von 127

1

Which microscopes are compound microscopes?

Wähle eine oder mehr der folgenden:

Phase/Contrast Microscope
Bright Field Microscope
Dark Field Microscope
Fluorescent Microscope
Scanning Microscope
Objective Microscope

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Frage 15 von 127

1

The ability of the microscope to enlarge an object.

Wähle eine der folgenden:

Magnification
Resolving power.
Refractive index.
Resolution.

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Frage 16 von 127

1

How clear your specimen will appear when viewed through the lenses.

Wähle eine der folgenden:

Resolution
Refractive index
Resolving power
Magnification

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Frage 17 von 127

1

How well you lens is able to bend light.

Wähle eine der folgenden:

Refractive index.
Resolution
Resolving power
Magnification

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Frage 18 von 127

1

What components influence the resolving power of a microscope?

Wähle eine oder mehr der folgenden:

Numerical aperture
Wavelength of light
Condenser
Diopter ring

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Frage 19 von 127

1

Concentrates the light beam and directs light through the specimen.

Wähle eine der folgenden:

Condenser
Iris diaphragm
Diopter ring
Refractive index

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Frage 20 von 127

1

Regulates the intensity of the light entering the lens.

Wähle eine der folgenden:

Iris diaphragm
Condenser
Diopter ring
Rheostat

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Frage 21 von 127

1

Focuses the individual eye piece to your eye.

Wähle eine der folgenden:

Diopter ring
Rheostat
Diaphragm ring
Iris diaphragm

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Frage 22 von 127

1

Steps to achieve adequate lighting when the oil immersion objective is used to view a specimen.

Wähle eine oder mehr der folgenden:

Condenser raised to the highest position
Iris and base diaphragm are open
Rheostat is turned up
Condenser is lowered to the lowest position

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Frage 23 von 127

1

Components that influence the resolving power of a microscope.

Wähle eine oder mehr der folgenden:

Numerical aperture
Wavelength of light
Rheostat level
Refractive index

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Frage 24 von 127

1

The isolation of a single species of microbe.

Wähle eine der folgenden:

Pure culture
Colony
Media
Inoculum
Turbdity

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Frage 25 von 127

1

Cells from a single asexual parent that are identical.

Wähle eine der folgenden:

Colony
Pure culture
Media
Inoculum
Turbidity

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Frage 26 von 127

1

Nutrient material suitable for the cultivation of microorganisms.

Wähle eine der folgenden:

Media
Pure culture
Colony
Turbdity
Inoculum

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Frage 27 von 127

1

Transfer of a pure culture from a slant culture to a new sterile broth. The sampling of a bacterial culture.

Wähle eine der folgenden:

Inoculum
Pure culture
Colony
Media
Turbidity

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Frage 28 von 127

1

Cloudiness in broth that indicates growth.

Wähle eine der folgenden:

Turbidity
Pure culture
Media
Colony
Inoculum

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Frage 29 von 127

1

Steps involved in labeling a test tube.

Wähle eine der folgenden:

Label a piece of labeling tape with your name and the name of the bacteria and place on test tube.
Use a wax pencil and label the test tube with your name and the name of the bacteria.
Use a marker and label the test tube with your name and the name of the bacteria.

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Frage 30 von 127

1

What are the steps for labeling a Petri plate?

Wähle eine der folgenden:

Label the bottom of the media side using a wax pencil or marker with your name and the organism you are transferring.
Label the lid using a wax pencil or marker with your name and the name of the organism you are transferring.
Label a piece of labeling tape with your name and the name of the organism you are transferring and place it on the lid of the Petri plate.

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Frage 31 von 127

1

What are the steps for incubating a Petri plate?

Wähle eine der folgenden:

Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side up.
Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side down.

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Frage 32 von 127

1

What is meant by the statement that microorganisms are ubiquitous?

Wähle eine der folgenden:

Microorganisms are everywhere
Microorganisms are hard to identify
Microorganisms are difficult to see without a microscope
Microorganisms come in many forms

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Frage 33 von 127

1

Handling of bacteria in a manner so as to prevent contaminating laboratory media and to prevent infecting ourselves and our classmates.

Wähle eine der folgenden:

Aseptic technique
Sterile transfer
Inoculation technique
Ubiquitous

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Frage 34 von 127

1

Why are "basic" dyes used in the Gram stain?

Wähle eine der folgenden:

Bacteria have an overall negative charge so stain with positive (basic) dyes. Opposites attract.
Bacteria have an overall positive charge so stain with positive (basic) dyes. Likes attract likes.

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Frage 35 von 127

1

State two differences between a simple stain and a differential stain.

Wähle eine der folgenden:

Simple stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Differential stain uses two dyes; different bacteria are stained contrasting colors; shows size, shape, arrangement and something else.
Differential stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Simple stain uses two dyes; different bacteria are stained contrasting colors for simple identification; shows size, shape, arrangement and something else.

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Frage 36 von 127

1

A) Has a high lipid content and thin layer of tecoic acid underneath and stains pink.
B) Has a low lipid content and thick layer containing tecoic acids and stains purple.

Wähle eine der folgenden:

A) Gram negative bacteria
B) Gram positive bacteria
A) Gram positive bacteria
B) Gram negative bacteria
A) Gram stain
B) Acid-fast stain
A) Acid-fast stain
B) Gram stain

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Frage 37 von 127

1

What us the importance of using only a pinpoint of inoculum for the smear preparation?

Wähle eine oder mehr der folgenden:

Insure proper staining
Insure proper decolorizing
The slide is small
Allows you to prepare only one or two bacteria.

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Frage 38 von 127

1

What is the importance of allowing the smear to air dry?

Wähle eine oder mehr der folgenden:

Insure the cells stay intact and stain properly.
Insure the cells are dead and stain properly
Insure the cells wont move around during staining

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Frage 39 von 127

1

What is the importance of decolorizing smears one at a time?

Wähle eine der folgenden:

It is the most critical step and there is no hard and fast rule on how long to decolorize.
It is the most critical step and don't want to drop the slides.
It is the most critical step and need to time it exactly.

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Frage 40 von 127

1

List the gram stain reagents in order and state their function.

Wähle eine der folgenden:

1) Crystal Violet - Primary stain
2) Iodine - Mordant, combines with the crystal violet in the cell.
3) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
4) Safranin - Counterstain or Secondary stain
1) Crystal Violet - Primary stain
2) Safranin - Counterstain or Secondary stain
3) Iodine - Mordant, combines with the crystal violet in the cell.
4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
1) Crystal Violet - Primary stain
2) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
3) Iodine - Mordant, combines with the crystal violet in the cell.
4) Safranin - Counterstain or Secondary stain
1) Crystal Violet - Primary Stain
2) Iodine - Mordant, combines with the crystal violet in the cell
3) Safranin - Counterstain or Secondary stain
4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer

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Frage 41 von 127

1

Why do gram negative cells lose the primary dye during decolorization?

Wähle eine der folgenden:

The high lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.
The low lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.

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Frage 42 von 127

1

Why is the Acid-fast stain considered a "differential stain"?

Wähle eine oder mehr der folgenden:

It distinguishes between two groups of organisms based on the lipid content in their cell walls.
It uses two positively charged dyes.
It uses one positively charged dye and one negatively charged dye.
It tells size, shape, arrangement and something else.
It tells size, shape and arrangement.

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Frage 43 von 127

1

What is the difference between the decolorizer used in the Gram stain and the decolorizer used in the Acid-fast stain?

Wähle eine der folgenden:

Acid fast uses a solution of 3% HCl and 95% ethanol. Gram stain uses just ethanol.
Acid fast uses a solution of 95% HCl and 3% ethanol. Gram stain uses just ethanol.

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Frage 44 von 127

1

Lipids in the cell's waxy wall prevent the acid-alcohol from decolorizing the cell.

Wähle eine der folgenden:

Acid-fast
Gram
Negative
Endospore

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Frage 45 von 127

1

What is the difference between the Ziehl-Neelsen stain and the Kinyoun stain?

Wähle eine der folgenden:

Ziehl-Neelsen uses steam to penetrate the mycolic acid in cell walls.
Kinyoun uses concentrated dye, hard heat fixing and longer exposure time.
Kinyoun uses steam to penetrate the mycolic acid in cell walls.
Ziehl-Neelsen uses concentrated dye, hard heat fixing and longer exposure time.
Ziehl-Neelsen uses hard heat fixing to penetrate the mycolic acid in cell walls.
Kinyoun uses concentrated dye, steam and longer exposure time.
Kinyoun uses hard heat fixing to penetrate the mycolic acid in cell walls.
Ziehl-Neelsen uses concentrated dye, steam and longer exposure time.

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Frage 46 von 127

1

Which are acid-fast organisms and what disease do they cause?

Wähle eine oder mehr der folgenden:

Mycobacterium tuberculosis
Disease: Tuberculosis
Mycobacterium leprae
Disease: Leprosy or Hansen's disease
Nocardia asteroides
Disease: Nocardiosis
Cryptosporidium
Disease: Cryptosporidiosis
Mycosporidium
Disease: Mycardiosis
Nocardia tuberculosis
Disease: Tuberculosis

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Frage 47 von 127

1

What are the reactants in the acid-fast stain, in order?

Wähle eine der folgenden:

1) Carbolfuchsin
2) Acid-alcohol
3) Loeffler's methylene blue
1) Loeffler's methylene blue
2) Acid-alcohol
3) Carbolfichsin
1) Carbolfuschsin
2) Iodine
3) Acid-alcohol
4) Loeffler's methylene blue
1) Loeffler's methylene blue
2) Iodine
3) Acid-alcohol
4) Carbolfuchsin

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Frage 48 von 127

1

Two methods used to force the dye into an endospore.

Wähle eine oder mehr der folgenden:

Steam for 3-5 minutes
Hard heat fixing then letting the dye remain on the slide for an extended period of time.
Hard heat fixing
Steam then letting the dye remain on teh slide for an extended period of time.

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Frage 49 von 127

1

What dyes are used in the Schaffer-Fulton spore stain?

Wähle eine oder mehr der folgenden:

Malachite green
Gram's safranin
Iodine
Loeffler's methylene blue

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Frage 50 von 127

1

In the "Christmas tree" stain, the endospores are colored _ and the vegetative cells are colored ____.

Wähle eine oder mehr der folgenden:

Green
Red
Red
Green

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Frage 51 von 127

1

Metabolically active, replicating cell.

Wähle eine oder mehr der folgenden:

Vegetative cell
Endospore
Germinated cell

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Frage 52 von 127

1

The formation of endospores within a vegetative cell

Wähle eine der folgenden:

Sporogenesis
Decolorization
Germination

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Frage 53 von 127

1

When an endospore reverts to a vegetative state.

Wähle eine der folgenden:

Germination
Sporogenesis
Growth

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Frage 54 von 127

1

Why are endospores considered "the most resistant life form known"?

Wähle eine oder mehr der folgenden:

Can remain dormant indefinitely
Resistant to antibiotics and most disinfectants
Resistant to radiation
Resistant to boiling
Resistant to drying
Impermeability of spore coat

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Frage 55 von 127

1

What are the medically important endospore-producing bacteria and their associated disease?

Wähle eine oder mehr der folgenden:

Bacillus anthracis
Disease: anthrax
Clostridium botulinum
Disease: Botulism
Clostridium difficile
Disease: Toxic enterocolotis
Clostridium perfringens
Disease: Gas gangrene, food poisoning
Clostridium tetani
Disease: Tetnus, lockjaw
Clostridium anthracis
Disease: Anthrax

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Frage 56 von 127

1

Which medically important genera of bacteria produce endospores?

Wähle eine oder mehr der folgenden:

Bacillus
Clostridium
Streptococcus
Staphylococcus

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Frage 57 von 127

1

A positive stain _ and a negative stain __.

Wähle eine der folgenden:

Stains what you want to see
Stains everything but the structure you want to see
Stains everything but the structure you want to see
Stains what you want to see
Stains gram (+) bacteria
Stains gram (-) bacteria
Stains gram (-) bacteria
Stains gram (+) bacteria

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Frage 58 von 127

1

Why are Nigrosin and India ink commonly used negative stains?

Wähle eine der folgenden:

They are negatively charged dyes and are repelled by the negative contents of the bacterial cell.
They are positively charged dyes and are repelled by the positive contents of the bacterial cell.
They are positively charged dyes and are attracted by the negative contents of the bacterial cell.
They are negatively charged dyes and are attracted by the positive contents of the bacterial cell.

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Frage 59 von 127

1

What are the advantages of using a negative stain versus a positive stain?

Wähle eine oder mehr der folgenden:

Able to see a capsule
Do not heat fix so can see them as they are
Can be used for things that don't stain.
Can see if it produces endospores
Can see if it is gram (-) or gram (+)

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Frage 60 von 127

1

Sticky substance that is tightly bound, highly organized and generally round or oval.

Wähle eine der folgenden:

Capsule
Slime layer
Endospore

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Frage 61 von 127

1

Sticky substance is loosely bound and somewhat irregularly shaped.

Wähle eine der folgenden:

Capsule
Slime layer
Endospore

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Frage 62 von 127

1

How does a capsule contribute to the virulence of a bacteria?

Wähle eine oder mehr der folgenden:

Helps the bacteria avoid phagocytosis by white blood cells
Helps the bacteria attach to skin and mucous membranes
Is a source of nutrition for the bacteria when resources are scarce
Contains the pathogenic part of the bacteria

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Frage 63 von 127

1

What are the difference(s) between the smear preparation for a Gram stain and a capsule stain?

Wähle eine oder mehr der folgenden:

A smear for a capsule stain is not heat fixed.
A spear from a capsule stain is not rinsed with deionized water it is rinsed with copper sulfate
A smear for a capsule stain uses steam
A smear for a Gram stain uses crystal violet and a capsule stain does not

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Frage 64 von 127

1

Using Anthony's stain, the background will be colored and the capsule will be colored _____.

Wähle eine der folgenden:

Dark blue
Light blue to white
Light blue to white
Dark blue

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Frage 65 von 127

1

What are the damaging by-products of oxygen during cellular respiration?

Wähle eine oder mehr der folgenden:

Superoxides
Peroxides
Carbon dioxide
Carbon monoxide

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Frage 66 von 127

1

What enzymes neutralize the damaging effects of the by-products of oxygen during cellular respiration?

Wähle eine oder mehr der folgenden:

Catalase
Peroxidase
Superoxide dismutase
Carbonase

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Frage 67 von 127

1

Uses oxygen as the final member of the ETC and has enzymes to break down the toxic by-products of oxygen.

Wähle eine der folgenden:

Aerobe
Anerobe

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Frage 68 von 127

1

Does not use oxygen as the final member of the ETC and lacks the enzymes to breakdown the toxic by-products of oxygen.

Wähle eine der folgenden:

Aerobe
Anaerobe

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Frage 69 von 127

1

How does a candle jar achieve an atmosphere to grow microaerophilic and capnophilic organisms?

Wähle eine der folgenden:

The flame burns using up the oxygen and producing CO2
The flame burns up the CO2 and produces oxygen.

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Frage 70 von 127

1

How does a Gas Pak jar achieve an anaerobic atmosphere?

Wähle eine der folgenden:

The pak contains chemicals that produce H+ and CO2. The H+ combines with the O2 in the jar to produce H2O.
The pak contains chemicals that produce CO2 and destroy O2.

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Frage 71 von 127

1

Which type of organism grows equally well in all types of oxygen concentrations?

Wähle eine der folgenden:

Facultative anaerobes
Microaerophiles
Capnophiles
Aerotolerant anaerobes

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Frage 72 von 127

1

How does thioglycolate broth grow anaerobic bacteria as well as aerobic bacteria?

Wähle eine oder mehr der folgenden:

The agar prevents diffusion of O2
It contains a reducing agent, sodium thioglycollate, that combines with O2 to produce water
It contains a reducing agent, sodium thioglycollate, that combines with the agar to produce CO2 near the bottom
The agar prevents diffusion of CO2

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Frage 73 von 127

1

Organisms that require around 20% atmospheric oxygen.
(Check the name of the organism and the media on which it will grow.)

Wähle eine oder mehr der folgenden:

Strict aerobe
Facultative anaerobe
Only at the top of the thioglycollate broth.
Candle jar
Gas pak
Capnophile

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Frage 74 von 127

1

Organisms that require between 5-15% atmospheric oxygen for growth
(Check the name of the organism and the media on which it will grow.)

Wähle eine oder mehr der folgenden:

Microaerophile
Facultative anaerobe
Grows in candle jar
Grows at the point where the oxygen level is diminishing in the thio broth.
Grows in the GasPak

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Frage 75 von 127

1

Organisms that require between 5-15% atmospheric oxygen and increased (10%) carbon dioxide.
(Check the name of the organism and the media on which it will grow.)

Wähle eine oder mehr der folgenden:

Capnophile
Microaerophile
Grows in candle jar
Grows in oxygen
Grows in a GasPak

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Frage 76 von 127

1

Organisms that will use a fermentative metabolic pathway in anaerobic conditions yet can convert to aerobic cellular respiration when atmospheric oxygen is present. They grow equally well in aerobic anaerobic environments.

Wähle eine oder mehr der folgenden:

Facultative anaerobe
Microaerophile
Grows in oxygen
Grows in candle jar
Grows in GasPak
Grows throughout the entire thio broth slant

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Frage 77 von 127

1

Organism that can exist in the presence of oxygen, but does not use oxygen for metabolism.

Wähle eine oder mehr der folgenden:

Aerotolerant anaerobe
Microaerophile
All over the thio broth slant but slightly less at the top
Grows in the GasPak
Capnophile

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Frage 78 von 127

1

Organisms that die in the presence of atmospheric oxygen because they lack the enzymes needed to breakdown the toxic by-products.

Wähle eine oder mehr der folgenden:

Strict anaerobe
Grows in GasPak
Grows in candle jar
Grows toward the bottom of the thio broth
Grows toward the top of the thio broth

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Frage 79 von 127

1

Which gene allows jellyfish to glow in the dark?

Wähle eine der folgenden:

Green fluorescent protein
Fluorescent blue plasmid
GFP
FBP

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Frage 80 von 127

1

Bacteria cells able to take up the plasmid/DNA.

Wähle eine der folgenden:

Competent cells
Competent genes
Competent proteins

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Frage 81 von 127

1

What laboratory procedures are done to encourage cells to become competent?

Wähle eine oder mehr der folgenden:

Mixing cells with calcium chloride during growth phase
Exposing cells to extreme temperature changes during the proceduree
Mixing cells with with heated calcium hydroxide
Freezing cells

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Frage 82 von 127

1

What is the relationship between a gene and a protein?

Wähle eine der folgenden:

A gene is a piece of DNA that provides the instructions for making a protein
A protein is a piece of DNA that provides the instructions for making a gene
DNA is a piece of a protein that provides the instructions for making a gene

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Frage 83 von 127

1

Chromosomal DNA _ and plasmids _______.

Wähle eine der folgenden:

Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.
Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.
Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.
Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.
Carries genes needed for the hereditary characteristics that may be beneficial but are not essential for bacterial growth and reproduction.
Small pieces of circular DNA that have genes for one or more traits that are essential for bacterial survival.

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Frage 84 von 127

1

Which genes are contained in the pGLO plasmid?

Wähle eine oder mehr der folgenden:

GFP
BLA
Ara-C
Ara-B

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Frage 85 von 127

1

How is expression of the Green Fluorescent Protein gene regulated?

Wähle eine der folgenden:

The Ara-C gene controls expression of GFP via the sugar arabinose
The Ara-B gene controls expression of GFP via the sugar arabinose
The Ara-C gene controls expression of GFP via the sugar glucose

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Frage 86 von 127

1

Chemicals produced by microorganisms that in small quantities can inhibit the growth of other microorganisms

Wähle eine der folgenden:

Antibiotic
MIC
E-test

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Frage 87 von 127

1

Determines the lowest concentration of an antibiotic that is able to inhibit the growth of a test organism.

Wähle eine der folgenden:

Antibiotic
MIC
E-test

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Frage 88 von 127

1

A plastic coated strip that contains a gradient

Wähle eine der folgenden:

Antibiotic
MIC
E-test

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Frage 89 von 127

1

What are the disadvantages of using a broad spectrum antibiotic?

Wähle eine oder mehr der folgenden:

Contributes to escalating drug resistance
Wipes out a person's normal flora resulting in superinfections
Only works on weak bacteria

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Frage 90 von 127

1

What does the antibiotic test result "sensitive" indicate about the test organism?

Wähle eine oder mehr der folgenden:

An organism is susceptible to drug concentrations obtained in the body using normal therapeutic doses.
The antibiotic is likely to be effective against the pathogen
The antibiotic is not likely to work.

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Frage 91 von 127

1

What factors must be controlled in order to standardize the Kirby-Bauer test?

Wähle eine oder mehr der folgenden:

Stability of the antibiotic
Rate of diffusion of the antibiotic
pH of the culture medium
Depth of the culture medium
Inoculum density
Incubation time
Incubation temperature
Concentration of antibiotic

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Frage 92 von 127

1

A?

Wähle eine der folgenden:

Arm
Rheostat
Power switch
Coarse focus knob

Erklärung

Frage 93 von 127

1

B?

Wähle eine der folgenden:

Rheostat
Power switch
Coarse focus knob
Arm

Erklärung

Frage 94 von 127

1

C?

Wähle eine der folgenden:

Rheostat
Power switch
Coarse focus knob
Fine focus knob

Erklärung

Frage 95 von 127

1

D?

Wähle eine der folgenden:

Coarse focus knob
Fine focus knob
RHeostat
Power switch

Erklärung

Frage 96 von 127

1

E?

Wähle eine der folgenden:

Fine focus knob
Coarse focus knob
Rheostat
Power switch

Erklärung

Frage 97 von 127

1

A?

Wähle eine der folgenden:

Ocular
Diopter
Diaphragm ring
Fine focus knob

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Frage 98 von 127

1

B?

Wähle eine der folgenden:

Mechanical stage
Base
Slide stage
Ocular stage

Erklärung

Frage 99 von 127

1

C?

Wähle eine der folgenden:

Fine focus knob
Coarse focus knob

Erklärung

Frage 100 von 127

1

D?

Wähle eine der folgenden:

Base
Mechanical stage
Mechanical base

Erklärung

Frage 101 von 127

1

A
B
C
D
E

Wähle eine der folgenden:

A - Objective lens
B - Iris diaphragm lever
C - Condenser centering screw
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Iris diaphragm lever
C - Condenser centering screw
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Condenser centering screw
C - Iris diaphragm lever
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Iris diaphragm lever
C - Mechanical stage knob
D - Base diaphragm
E - Condenser centering screw

Erklärung

Frage 102 von 127

1

A
B
C

Wähle eine der folgenden:

A - Iris diaphragm lever
B - Condenser centering screw
C - Base diaphragm
A - Condenser centering screw
B - Iris diaphragm lever
C - Base diaphragm
A - Iris diopter screw
B - Iris diaphragm lever
C - Base diaphragm
A - Iris diopter lever
B - Condenser centering screw
C - Base diaphragm

Erklärung

Frage 103 von 127

1

A
B

Wähle eine der folgenden:

A - Revolving nose piece
B - Mechanical stage clip
A - Objective ring
B - Mechanical stage clip
A - Diopter ring
B - Mechanical stage clip

Erklärung

Frage 104 von 127

1

A
B
C

Wähle eine der folgenden:

A - Condenser knob
B - Condenser
C - Base diaphragm ring
A - Condenser knob
B - Condenser ring
C - Base
A - Condenser ring
B - Condenser
C - Base

Erklärung

Frage 105 von 127

1

In the thioglycollate test tube A, what kind of organism does the growth represent?

Wähle eine der folgenden:

Microaerophile
Obligate/Strict aerobe
Facultative anaerobe
Obigate/strict anaerbe

Erklärung

Frage 106 von 127

1

In the thioglycollate test tube B, what kind of organism does the growth represent?

Wähle eine der folgenden:

Microaerophile
Obligate/Strict aerobe
Facultative anaerobe
Obligate anaerobe

Erklärung

Frage 107 von 127

1

In the thioglycollate test tube C, what kind of organism does the growth represent?

Wähle eine der folgenden:

Microaerophile
Obligate/strict aerobe
Facultative anaerobe
Obligate/strict anaerobe

Erklärung

Frage 108 von 127

1

In the thioglycollate test tube D, what kind of organism does the growth represent?

Wähle eine der folgenden:

Microaerophile
Obligate/strict aerobe
Facultative anaerobe
Obligate/strict anaerobe

Erklärung

Frage 109 von 127

1

On a luria plate with Ampicillin, why is there no E. coli growth?

Wähle eine der folgenden:

Because E.coli are sensitive to Ampicillin
Because E. coli are resistant to Ampicillin
Because luria does not contain the nutrients necessary for growth.

Erklärung

Frage 110 von 127

1

What protein allowed E. coli to grow on the plate with luria, Ampicillin and pGlo?

Wähle eine der folgenden:

Beta lactamase
BLA
GFP
Green fluorescent protein

Erklärung

Frage 111 von 127

1

What gene allowed the E. coli to grow on the plate with luria, Ampicillin and pGlo?

Wähle eine der folgenden:

BLA
Beta lactamase
GFP
Green fluorescent protein

Erklärung

Frage 112 von 127

1

What molecule allowed the E. coli to glow like a jellyfish?

Wähle eine der folgenden:

Arabinose
Beta lactamase
Ara-C
Green fluorescent protein

Erklärung

Frage 113 von 127

1

What media is used for the antibiotic sensitivity test?

Wähle eine der folgenden:

Mueller hinton
Kirby Bauer
Luria

Erklärung

Frage 114 von 127

1

What is the result of the beta lactamase test pictured?

Wähle eine der folgenden:

It is pink and therefore, the test is negative and the microbe is sensitive to penicillin
it is pink and therefore, the test is positive and the microbe is sensitive to penicillin
it is pink and therefore, the test is positive and the microbe is resistant to penicillin
It is pink and therefore, the test is negative and the microbe is resistant to penicillin

Erklärung

Frage 115 von 127

1

Beta lactamase test

Wähle eine der folgenden:

It is yellow and therefore, the test is positive and the microbe is resistant to penicillin
It is yellow and therefore, the test is negative and the microbe is resistant to penicillin
It is yellow and therefore, the test is positive and the microbe is sensitive to penicillin
It is yellow and therefore, the test is negative and the microbe is sensitive to penicillin

Erklärung

Frage 116 von 127

1

Why us thioglycollate a semi-solid agar?

Wähle eine der folgenden:

Has agar in it and contains a reducing agent that produces water
Has agar and a reducing agent that produces CO2

Erklärung

Frage 117 von 127

1

Why are agar plates labeled on the bottom and inverted when incubated?

Wähle eine der folgenden:

Labeled on the bottom in case you lose the lid and inverted during incubation to prevent condensation from dripping onto the agar surface
Labeled on the bottom in case you lose the lid and inverted so you can read the label

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Frage 118 von 127

1

Why hold the test tube with your little finger while taking your bacterial sample?

Wähle eine der folgenden:

So you do not contaminate the cap.
So the cap doesn't roll away.
So you can stretch your fingers.

Erklärung

Frage 119 von 127

1

Describe the proteins encoded in the pGLO plasmid.

Wähle eine oder mehr der folgenden:

Green fluorescent protein
Beta lactamase
Ara-C represser protein
Beta green protein

Erklärung

Frage 120 von 127

1

What is the principal of the beta lactamase test?

Wähle eine der folgenden:

Does the bacteria have the BLA gene and therefore produce beta lactamase
Does the bacteria have the BLA protein and therefore produce the beta lactamase gene

Erklärung

Frage 121 von 127

1

Explain the regulation mechanism of the Green Fluorescent Protein gene expression.

Wähle eine der folgenden:

Arabinose has to move the Ara-C represser protein out of the way so GFP can be transcribed and translated.
GFP takes up the arabinose so Ara-C can be transcribed and translated.

Erklärung

Frage 122 von 127

1

Define reducing agent

Wähle eine der folgenden:

Sodium thioglycollate is a reducing agent and it reduces by binding O2 and converting it to water
Sodium thioglycollate is a reducing agent that reduces by causing evaporation of the broth

Erklärung

Frage 123 von 127

1

What are the criteria used to describe a bacterial colony>

Wähle eine oder mehr der folgenden:

Surface
Elevation
Whole colony
Edge
Color

Erklärung

Frage 124 von 127

1

Locations of items in the lab

Wähle eine oder mehr der folgenden:

Near the front door: Fire extinguisher, Eye wash, Shower, Gloves
Back of room: Fume hood
Lab bench: Disinfectant, Autoclave bags, Paper towels
Near Sinks: Bleach, Dyes used for stains, Paper towels (above)
Fume hood: Broken glass disposal box (underneath), Autoclave bags
Front podium: Phone
Back: Test tube racks, MSDS notebooks (back corner),
Next to fridge: Dustpan and broom

Erklärung

Frage 125 von 127

1

Which items are disposed of in the autoclave trash?

Wähle eine oder mehr der folgenden:

Used glass test tubes
Used glass slides
Broken glass
Used latex gloves
Paper towels used for bench disinfection
Contaminated Petri plates

	Erstellt von Elle Ashe vor fast 11 Jahre

MCC Microbiology Lab Practical 2

Microbiology Lab Practical

Frage 1 von 127

What method of staining was used to create this slide?

Wähle eine der folgenden:

Erklärung

Frage 2 von 127

What kind of staining process was used to make this slide?

Wähle eine der folgenden:

Erklärung

Frage 3 von 127

What kind of microbes are present on this slide?

Wähle eine der folgenden:

Erklärung

Frage 4 von 127

Where should your backpacks and purses be stored during the lab period?

Wähle eine der folgenden:

Erklärung

Frage 5 von 127

What items should you wear to protect yourself in the lab.

Wähle eine oder mehr der folgenden:

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Frage 6 von 127

When do you disinfect your lab table and where do you discard the used paper towels?

Wähle eine oder mehr der folgenden:

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Frage 7 von 127

You are dying of thirst (hunger, for smoke, chapped lips, bad breath) during the lab period. What do you do to alleviate your problem?

Wähle eine der folgenden:

Erklärung

Frage 8 von 127

You notice your lab partner has caught the lab manual on fire. What should you do?

Wähle eine oder mehr der folgenden:

Erklärung

Frage 9 von 127

Your lab partner has managed to spill microorganisms all over the lab table. What should be done to clean up the spill?

Wähle eine oder mehr der folgenden:

Erklärung

Frage 10 von 127

A student broke an empty glass test tube. What should be done to clean up the glass and where should the pieces of glass be disposed of?

Wähle eine oder mehr der folgenden:

Erklärung

Frage 11 von 127

The instructor tripped on a backpack on the floor knocking her safety goggles off and splashing HCl in her eyes. What should she do?

Wähle eine oder mehr der folgenden:

Erklärung

Frage 12 von 127

After surviving the lab period you remove your gloves by turning them inside out and then where do you toss them?

Wähle eine oder mehr der folgenden:

Erklärung

Frage 13 von 127

Choose the two major types of microscopes that have been developed and the difference between them

Wähle eine oder mehr der folgenden:

Erklärung

Frage 14 von 127

Which microscopes are compound microscopes?

Wähle eine oder mehr der folgenden:

Erklärung

Frage 15 von 127

The ability of the microscope to enlarge an object.

Wähle eine der folgenden:

Erklärung

Frage 16 von 127

How clear your specimen will appear when viewed through the lenses.

Wähle eine der folgenden:

Erklärung

Frage 17 von 127

How well you lens is able to bend light.

Wähle eine der folgenden:

Erklärung

Frage 18 von 127

What components influence the resolving power of a microscope?

Wähle eine oder mehr der folgenden:

Erklärung

Frage 19 von 127

Concentrates the light beam and directs light through the specimen.

Wähle eine der folgenden:

Erklärung

Frage 20 von 127

Regulates the intensity of the light entering the lens.

A) Has a high lipid content and thin layer of tecoic acid underneath and stains pink.
B) Has a low lipid content and thick layer containing tecoic acids and stains purple.