Genetics

Frage 1 von 32

1

Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?

Wähle eine der folgenden:

Restriction fragment length polymorphisms
Short tandem repeats
Variable number tandem repeats
Single nucleotide polymorphisms
Chromosomal translocation

Erklärung

Frage 2 von 32

1

Which of the following polymorphisms can be detected through Southern blot?

Wähle eine der folgenden:

Short tandem repeats
Single nucleotide polymorphisms
Restriction fragment length polymorphisms
Long interspersed nucleotide sequences

Erklärung

Frage 3 von 32

1

What does it mean, that a locus is homozygous?

Wähle eine der folgenden:

many different alleles
two alleles that are the same
two alleles that are different
no alleles
one allele have been deleted

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Frage 4 von 32

1

The following is a description of which method.
Patients DNA was isolated and cut with HaeIII. Product of the restriction digest was resolved by gel electrophoresis and transferred to a membrane. Then the membrane was exposed to hybridization probes, and the resulting band pattern was compared with that seen in another sample of DNA.

Wähle eine der folgenden:

RFLP by Southern blotting
VNTR by PCR
SNP by sequencing
STR by PCR
SNP by Western Blot

Erklärung

Frage 5 von 32

1

FGA, D8S1179, and D2S11 are examples of which of the following?

Wähle eine der folgenden:

RFLP
VNTR
STR
SNP

Erklärung

Frage 6 von 32

1

Which of the following is used as a marker of gender?

Wähle eine der folgenden:

D8S1179
Amelogenin
TPOX
PentaD
CD4

Erklärung

Frage 7 von 32

1

How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?

Wähle eine der folgenden:

10
12
13
16
20

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Frage 8 von 32

1

When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?

Wähle eine der folgenden:

1
2
4
6
25

Erklärung

Frage 9 von 32

1

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Wähle eine der folgenden:

STR
Y-STR
RFLP
VNTR
SNP

Erklärung

Frage 10 von 32

1

Which of the following STRs is a Y-STR?

Wähle eine der folgenden:

D5S818
vWA
Penta E
DYS385
LPL

Erklärung

Frage 11 von 32

1

A child has disputed paternity. The child has the following STR haplotype:
TPOX = 7/8; D5S818 = 11/12; D13S317 = 9/10; vWA = 11/12; D21S11 = 27/28.
Alleged Father 1 has the following STR haplotype: TPOX = 6/10; D5S818 = 9/13; D13S317 = 9/14; vWA = 14/16; D21S11 = 30/33.
What can be said of Alleged Father 1?

Wähle eine der folgenden:

He is the father of the child
He cannot be excluded as the father
He is excluded as the father.
More STRs need to be analyzed before a conclusion can be made.

Erklärung

Frage 12 von 32

1

An individual who has two populations of cells that were derived from two different zygotes is called a:

Wähle eine der folgenden:

chimera
mosaic
heteroplasmy
mutant

Erklärung

Frage 13 von 32

1

A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

Wähle eine der folgenden:

RFLP
STR
Y-STR
VNTR

Erklärung

Frage 14 von 32

1

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

Wähle eine der folgenden:

Female
Male
Impossible to tell from one locus
This locus does not discriminate between males and females

Erklärung

Frage 15 von 32

1

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

Wähle eine der folgenden:

To determine whether the donor and recipient are a match
To determine the likelihood of graft-versus-host disease
To find informative alleles for post-transplantation studies
To add their STR haplotypes to a national database

Erklärung

Frage 16 von 32

1

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Wähle eine der folgenden:

vWA and TH01
TH01 and D13S317
D16S539 and F13A01
vWA and D13S317

Erklärung

Frage 17 von 32

1

Bone marrow has been transplanted into a recipient. STR analysis was performed on whole blood from the recipient 6 months after the transplant by PCR, followed by capillary gel electrophoresis with fluorescence measurement of amplicons. For the TPOX locus, the donor and recipient do not share alleles. The fluorescence of the TPOX locus for the donor peak was 15693, and the fluorescence of the TPOX locus for the recipient peak was 5329. What is the percentage of recipient cells remaining?

Wähle eine der folgenden:

25%
34%
66%
75%

Erklärung

Frage 18 von 32

1

Two people have what percentage of DNA sequence that differs between them?

Wähle eine der folgenden:

1%
5%
10%
25%
15%

Erklärung

Frage 19 von 32

1

Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

Wähle eine der folgenden:

Y-STR
Mitochondrial DNA
RFLP-PCR
SNP haplotypes

Erklärung

Frage 20 von 32

1

Consider the following STR analysis:
Which of the child alleles are inherited form the father?

Wähle eine der folgenden:

D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 9
D3S1358: 4, vWA: 5, FGA: 20, TH01: 11 , TPOX: 10, CSF1PO: 13, D5S818: 3, D13S317: 6
D3S1358: 15, vWA: 17, FGA: 23, TH01: 6 , TPOX: 11, CSF1PO: 12, D5S818: 10, D13S317: 10
D3S1358: 10, vWA: 17, FGA: 24, TH01: 6 , TPOX: 10, CSF1PO: 12, D5S818: 10, D13S317: 9
D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 10

Erklärung

Frage 21 von 32

1

Basked on the results which of the alleged fathers (AF) is the father?

Wähle eine der folgenden:

AF1
AF2
Neither

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Frage 22 von 32

1

A child and an alleged father (AF) share alleles with the following paternity index:
What is the combined paternity index from these three loci?

Wähle eine der folgenden:

4.131
4.5
5
3.131

Erklärung

Frage 23 von 32

1

With 50% prior odds, what is the probability of paternity from these three loci?

Wähle eine der folgenden:

80%
76%
90%
60%

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Frage 24 von 32

1

What is a traditional definition of a polymorphism?

Wähle eine der folgenden:

The genets variation must be present in at least 1 % of the population.
The genets variation must be present in at least 5 % of the population.
The genets variation that no longer is present in the population.
The genets variation can not be present in majority of the population.

Erklärung

Frage 25 von 32

1

Which of the following genomic variations is the hardest to detect?

Wähle eine der folgenden:

SNP
STRs
VNTRs
RFLPs

Erklärung

Frage 26 von 32

1

When comparing a generic similarity go two random individuals, what percentage of their genome is identical?

Wähle eine der folgenden:

99.5%
95%
90%
100%
80%

Erklärung

Frage 27 von 32

1

RFLP stands for?

Wähle eine der folgenden:

Restriction fragment length polymorphism
Rapidly Fusing length polymorphisms
Rescue factor logical polymorphisms
Redundant fragment length polymorphism

Erklärung

Frage 28 von 32

1

Closely linked markers are used for:

Wähle eine der folgenden:

Accurate mapping of regions associated with phenotypic characteristics
Finding the length of a sequence
Location of the centromere
Defining the promoter
Disabling the binding of transcription factors

Erklärung

Frage 29 von 32

1

What is a definition of a haplotype?

Wähle eine der folgenden:

A block of closely linked SPS on the same chromosome that tend to be inherited together.
A half of a chromosome.
Chromosome with only one functioning allele.
Allele that gives a disfiguring phenotype.

Erklärung

Frage 30 von 32

1

Mitochondrial DNA is____

Wähle eine der folgenden:

circular double stranded
circular single stranded
linear double stranded
linear single stranded

Erklärung

Frage 31 von 32

1

Genes encoded on the mtDNA include:

Wähle eine der folgenden:

22 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
30 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 6 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 2 ribosomal RNA genes, and 60 genes coding for components of the oxidation-phosphorylation system.

	Erstellt von mona1nyc vor fast 11 Jahre

DNA Polymorphisms and Human Identification MB ASCP

Frage 1 von 32

Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?

Wähle eine der folgenden:

Erklärung

Frage 2 von 32

Which of the following polymorphisms can be detected through Southern blot?

Wähle eine der folgenden:

Erklärung

Frage 3 von 32

What does it mean, that a locus is homozygous?

Wähle eine der folgenden:

Erklärung

Frage 4 von 32

Wähle eine der folgenden:

Erklärung

Frage 5 von 32

FGA, D8S1179, and D2S11 are examples of which of the following?

Wähle eine der folgenden:

Erklärung

Frage 6 von 32

Which of the following is used as a marker of gender?

Wähle eine der folgenden:

Erklärung

Frage 7 von 32

How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?

Wähle eine der folgenden:

Erklärung

Frage 8 von 32

When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?

Wähle eine der folgenden:

Erklärung

Frage 9 von 32

Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?

Wähle eine der folgenden:

Erklärung

Frage 10 von 32

Which of the following STRs is a Y-STR?

Wähle eine der folgenden:

Erklärung

Frage 11 von 32

Wähle eine der folgenden:

Erklärung

Frage 12 von 32

An individual who has two populations of cells that were derived from two different zygotes is called a:

Wähle eine der folgenden:

Erklärung

Frage 13 von 32

A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?

Wähle eine der folgenden:

Erklärung

Frage 14 von 32

DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?

Wähle eine der folgenden:

Erklärung

Frage 15 von 32

STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?

Wähle eine der folgenden:

Erklärung

Frage 16 von 32

STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?

Wähle eine der folgenden:

Erklärung

Frage 17 von 32

Wähle eine der folgenden:

Erklärung

Frage 18 von 32

Two people have what percentage of DNA sequence that differs between them?

Wähle eine der folgenden:

Erklärung

Frage 19 von 32

Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?

Wähle eine der folgenden:

Erklärung

Frage 20 von 32

Consider the following STR analysis: Which of the child alleles are inherited form the father?

Wähle eine der folgenden:

Erklärung

Frage 21 von 32

Consider the following STR analysis:
Which of the child alleles are inherited form the father?

A child and an alleged father (AF) share alleles with the following paternity index:
What is the combined paternity index from these three loci?