What type of tissue was used in this organ bath?

What type of muscle is found in the bladder? How is its function controlled?

Why is it important that smooth muscle does not exhibit the 'twitch' function seen in skeletal muscle?

Which receptor type and neurotransmitter is involved in the control of the detrusor muscle?

What is a common muscarinic receptor antagonist?

What type of relationship can be measured between the concentration of the drug added to the organ bath and the response recorded?

Did you use a PowerLab in this experimental setup?

Why did you have to calibrate the force transducer?

What proportions of O2 and CO2 did you supply the tissue with in the organ bath?

At what rate did you supply the tissue with O2/CO2?

How did you equilibrate the tissue? What was the point of this?

How did you prepare your dilutions from the 10^-2 M solution of acetylcholine?

How did you add half measures of each dilution to the organ bath e.g. 5x10^-6 M?

Why does pig bladder tissue react more slowly to acetylcholine than the rat ilium used in the organ bath prac last year?

What was the timing schedule for the assay (just acetylcholine)?

What differed between the method in the first practical (just acetylcholine) and the second?

How was the second practical a double-blind experiment?

Which muscarinic receptor antagonists were used and which receptor subtype did they antagonise?

What were the acetylcholine solutions used in the second practical?

What were the main activities in parts 1 and 2 of the second practical?

What is the major subtype in the muscarinic receptor population found in the pig detrusor muscle (the largest proportion, not necessarily the largest contributor to function)?

What is the EC50?

Affinity constants (pKb) can be calculated in order to give pharmacologists an idea of how selective an antagonist/agonist is for a certain receptor or receptor subtype. How is pKb calculated?

What was the main finding of the double blind second practical?