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Direct cell counts can be divided into 2. Total count and viable count
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Total count measure the total number of dead cells
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A bacillus bacteria is spherical
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A cocci bacteria is round
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Before gram staining bacteria is colourless
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Gram negative bacteria is susceptible to antibiotics and antibacterial agents
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The gram positive cell wall is more complex than gram negative's cell wall
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Aseptic refers to a procedure performed under sterile conditions
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Gram negative has a thin layer of peptidoglycan
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A pathogen is a disease causing micro-organism
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A colony of bacteria arises from more than one bacteria
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An air filter bubbles air through the fermenter
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A secondary metabolite is produced after an exponential growth of fungus is complete
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Other methods of measuring bacteria growth includes haemocytometer and turbidmetry
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Facultative anaerobes grow better in presence of oxygen however they can also grow in absence of oxyegen
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Optimum temperature for mammalian pathogens is 25 degrees celsius.
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Radiation such as gamma rays is used to sterile plastic equipment
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Glass cannot be sterilised in an autoclave
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Gram negative bacteria is a purple colour after staining because it has a thick layer of peptidoglycan
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After sterilising the work surface, the cap of the culture bottle can be placed down
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Seal petri dish and lid all the way around
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Sterilise all equipment in boiling water for at least 15 minutes before use.
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After innoculation leave the lid of the petri dish for a few minutes