16253851
Beschreibung
Quiz von Olivia Muench, aktualisiert more than 1 year ago
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Erstellt von Olivia Muench
vor etwa 6 Jahre
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Frage 1
Frage
Microbiology Lab is Biosafety Level 2
Antworten
- True
- False
Frage 2
Frage
Bacteria are [blank_start]ubiquitous[blank_end], since they can be found all over and in every environment.
Antworten
-
ubiquitous
-
formite
-
thick
Frage 3
Frage
Bacteria have a [blank_start]negative charge[blank_end]. Because of this basic dyes, with their [blank_start]positive charge[blank_end], stain the [blank_start]bacteria[blank_end]. Acidic dyes, with their [blank_start]negative charge[blank_end], stain the [blank_start]background[blank_end].
Antworten
-
negative charge
-
positive charge
-
positive charge
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negative charge
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negative charge
-
positive charge
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bacteria
-
background
-
background
-
bacteria
Frage 4
Frage
Examples of acidic dyes:
Antworten
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Eosin
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Methylene Blue
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Crystal Violet
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Nigrosin
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Safranin
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Congo Red
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Maneval's stain
Frage 5
Frage
Which microscope was used in the lab?
Antworten
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Dark-field microscope
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Phase contrast microscope
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Bright-field microscope
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Flourescence microscope
Frage 6
Frage
Limit of Resolution:
D = [blank_start]Wavelength[blank_end] / ([blank_start]NA condensor[blank_end] + [blank_start]NA objective lens[blank_end])
Antworten
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Wavelength
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NA condensor
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NA objective lens
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Wavelength
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NA condensor
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NA objective lens
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Wavelength
-
NA condensor
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NA objective lens
Frage 7
Frage
Smears should:
Antworten
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withstand washing
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cover the entire slide
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not be too thick
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not result in excessive distortion of the organism
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fall off the slide
Frage 8
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Purpose of heat-fixing
Antworten
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kill bacteria
-
kill you
-
fix the bacteria to the slide
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make cells more visible by coagulating cytoplasmic proteins
Frage 9
Frage
Pure cultures should have many different kinds of bacteria
Antworten
- True
- False
Frage 10
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Klebsiella Pneumoniae has a capsule
Antworten
- True
- False
Frage 11
Frage
Choose what best describes capsules
Antworten
-
Made of a polysaccharide layer called glycocalyx
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Water soluble
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Fat soluble
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Heat sensitive
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Prevents dehydration/dessication
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Decreases virulence by protecting the cell from phagocytosis
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Helps attachment to host cell
Frage 12
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Gram-positive bacteria
Antworten
-
Have an outer membrane/lipopolysaccharide layer (LPS)
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Cell wall is ~90% peptidoglycan and has teichoic acid
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Cell wall is ~10% peptidoglycan
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are stained pink/red with safranin
Frage 13
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Order of Gram staining:
1. [blank_start]Primary stain[blank_end] with [blank_start]Crystal Violet[blank_end]
2. [blank_start]Mordant[blank_end] with [blank_start]Gram's Iodine[blank_end]
3. [blank_start]Decolorizer[blank_end] with [blank_start]95% Ethanol[blank_end]
4. [blank_start]Counterstain[blank_end] with [blank_start]Safranin[blank_end]
Antworten
-
Primary stain
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Crystal Violet
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Mordant
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Gram's Iodine
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Decolorizer
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95% Ethanol
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Counterstain
-
Safranin
Frage 14
Frage
Which describes chemotaxis?
Antworten
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organism vibrates due to molecules hitting the cell
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directional; independent movement over greater distances
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movement towards chemical attractants and away from repellants
Frage 15
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Which describes lophotrichous flagella?
Antworten
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flagella on both ends
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flagella around perimeter of bacteria
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a single flagella at one end
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a tuft of flagella at one end
Frage 16
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The motility test media is 0.4-0.5% agar and contains TTC (2,3,5-triphenyltetrazolium chloride)
Antworten
- True
- False
Frage 17
Frage
Disadvantages of the motility test:
Antworten
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can be used to test for motility of pathogenic organisms
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requires incubation time
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if not performed properly, non-motile organisms appear motile
Frage 18
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TTC is colorless when [blank_start]oxidized[blank_end] and red when [blank_start]reduced[blank_end].
Antworten
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oxidized
-
reduced
Frage 19
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Escherichia coli is motile and Staphylococcus epidermidis is non-motile.
Antworten
- True
- False
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