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How do we study microbes and all the jazz?
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Culture them
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Ingest them
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Look at them under a microscope
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Study their DNA, RNA, and proteins!
Frage 2
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Complex media is harder to make and every single ingredient is measured.
Frage 3
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General Media has which of the following characteristics?
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Super selective
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Great Plate Count Anomaly
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Still can’t culture everything
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Cultures as many microbes as possible
Frage 4
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Enrichment media has which of the following characteristics/example(s)?
Frage 5
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[blank_start]Selective[blank_end] media selects for certain bacteria and selects against other bacteria.
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Selective
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Enriched
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Differential
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General
Frage 6
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Differential media usually is dependent on bacterial metabolic activity.
Frage 7
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Differential media is not similar or at all like selective media.
Frage 8
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Choose the correct terms for the following characteristics of autoclaving.
Autoclaving uses high [blank_start]pressure[blank_end] and high temperature to kill even [blank_start]endospores[blank_end].
[blank_start]Wet[blank_end] heat has more energy than dry heat. This kills everything but can be [blank_start]bad[blank_end] for delicate or reactive compounds. Autoclaving can also cause [blank_start]vitamins[blank_end] to [blank_start]denature[blank_end]. Also, trace metals that are [blank_start]redox[blank_end]-sensitive might react and become unavailable.
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pressure
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endospores
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Wet
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bad
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vitamins
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denature
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redox
Frage 9
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If autoclaving can denture vitamins and ruin or cause trace metal react, then what is another method of sterilization that would be better suited to handle this problem?
Frage 10
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Which of the following is TRUE about filtered sterilization?
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Used for reactive compounds (vitamins or trace metals)
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Removes the cells from the liquid, as autoclaving kills cells but doesn’t necessarily destroy them.
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Requires UV lamp
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Need cell-free water to do dilutions for microscope counts
Frage 11
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Which is not a form of UV light sterilization?
Frage 12
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Order the strength of UV light sterilization in increasing order in terms of effectiveness of killing microbes:
1) Lowest --> [blank_start]UV-A[blank_end]
2) Medium --> [blank_start]UV-B[blank_end]
3) Highest --> [blank_start]UV-C[blank_end]
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UV-A
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UV-B
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UV-C
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UV-B
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UV-A
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UV-C
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UV-C
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UV-A
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UV-B
Frage 13
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UV-A is the least effective in killing microbes but has the deepest penetrance. UV-C is the most effective in killing microbes but has the lowes penetrance.
Frage 14
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Hypothermiphiles and thermophiles grow in [blank_start]hot[blank_end] environments.
Psychrophiles grow in [blank_start]cold[blank_end] environments.
Halophiles love [blank_start]salt[blank_end].
Osmophiles love [blank_start]sugar[blank_end].
Xerophiles grow in [blank_start]dry[blank_end] environments.
Frage 15
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What is true about thermophiles?
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They love cold environments
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They have unsaturated fatty acids
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They have saturated fatty acids
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They use unsaturated fatty acids to increase the melting temperature of the cell membrane.
Frage 16
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The closer to 1, the purer the water solution.
Frage 17
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Why do we use salt and sugar in food preservation?
Frage 18
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We can use microbes to preserve our food by them producing acid. This method of food preservation is called?
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Fermentation
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Salting
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Sugaring
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Acidification
Frage 19
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Canned fruits naturally have higher acid than canned meat or vegetables as they have naturally occurring acidity prevents the growth of this bacteria.
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E. coli
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C. botulinum
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Listeria
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Anaerobes
Frage 20
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What is pasteurization?
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Heating food at a HIGH temperature for a LONG time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
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Heating food at a LOW temperature for a SHORT time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
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Heating food at a HIGH temperature for a SHORT time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
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Heating food at a LOW temperature for a LONG time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
Frage 21
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What is true about anaerobes?
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Boiling water to remove gas is needed to make anaerobic media.
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Anaerobes need air to survive
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Some obligate anaerobes have superoxide reductase
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Obligate anaerobes can thrive in the presence of oxygen
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They cannot utilize oxygen as an electron acceptor
Frage 22
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Which type of organism contains a protein-free manganese complex instead of superoxide dismutase?
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Aerobes
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Microaerophiles
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Aerotolerant
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Obligate anaerobes
Frage 23
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Aerobes and facultative aerobes always have [blank_start]superoxide dismutase[blank_end] and [blank_start]catalase[blank_end].
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superoxide dismutase
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catalase
Frage 24
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Some aerotolerant organisms have [blank_start]protein-free manganese complexes[blank_end] instead of [blank_start]superoxide dismutase[blank_end]
Frage 25
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Obligate anaerobes have [blank_start]superoxide[blank_end] [blank_start]reductase[blank_end] instead of [blank_start]superoxide[blank_end] [blank_start]dismutase[blank_end].
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superoxide
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reductase
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superoxide
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dismutase
Frage 26
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Which of these organisms utilize oxygen as a terminal electron acceptor?
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Aerotolerant
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Aerobes
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Facultative aerobes
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Microaerophiles
Frage 27
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Bacteriocidal [blank_start]kills[blank_end] the bacteria, bacteriostatic [blank_start]stops[blank_end] bacterial growth, and bacteriolytic [blank_start]destroys[blank_end] the cells.
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kills
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destroys
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breaks down
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stops
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grows
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slows
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destroys
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hides
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nurse
Frage 28
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[blank_start]Sterilants[blank_end] destroy all microorganisms including endospores
[blank_start]Disinfects[blank_end] are used on surfaces to kill microorganisms but not necessarily endospores
[blank_start]Sanitizers[blank_end] reduce microbial numbers but do not sterilize
[blank_start]Antiseptics[blank_end] kill or inhibit microbial growth, but are non-toxic enough to be applied to living tissue
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Sterilants
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Disinfects
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Sanitizers
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Antiseptics