Bacterial growth and control

Beschreibung

undergraduate Microbiology Quiz am Bacterial growth and control, erstellt von Beth Moore am 22/05/2015.
Beth Moore
Quiz von Beth Moore, aktualisiert more than 1 year ago
Beth Moore
Erstellt von Beth Moore vor etwa 9 Jahre
185
6

Zusammenfassung der Ressource

Frage 1

Frage
Which of the following is NOT an environmental parameter that limits bacterial growth?
Antworten
  • Temperature
  • pH
  • Metabolism
  • Osmotic pressure
  • None of the above

Frage 2

Frage
How is a psychrophile adapted to deal with extreme temperatures?
Antworten
  • Increased number of saturated fatty acids
  • Short acetyl chains on fatty acids
  • Increased G-C content
  • Produces anti-freeze proteins
  • All of the above

Frage 3

Frage
Which of the following is true of cryoprotectants?
Antworten
  • Bind to small ice crystals and prevent cell lysis
  • Insert into the plasma membrane to make it more fluid
  • A good example is Trehalose and exopolysaccharides
  • All of the answers

Frage 4

Frage
What would you expect an Alkaliphile to use to drive cell processes?
Antworten
  • Protons
  • Potassium ions
  • Sodium ions
  • Calcium Chloride

Frage 5

Frage
What is a thermosome?
Antworten
  • An bacterium that thrives in hot temperatures
  • A thermostable protein
  • A chaperonin protein complex
  • A glycerol tetraether

Frage 6

Frage
Which of the following is not mechanism by which halotolerant and halophilic bacteria respond to osmotic stress?
Antworten
  • Release solutes through mechanosensitive channels
  • Stabilisation of S-layer glycoproteins with sodium ions
  • Production of compatible solutes inside the cell
  • Modification of mitochondrial metabolism

Frage 7

Frage
Which of the following is needed to catalyse the whole of this reaction?
Antworten
  • Peroxidase
  • Superoxidase dismutase + catalase
  • catalase
  • Superoxidase dismutase + superoxidase reductase

Frage 8

Frage
Which of the following categories of bacteria contain catalase + SOD ?
Antworten
  • Facultative aerobes
  • Obligate aerobes
  • Obligate anaerobes
  • Aerotolerant aerobes
  • Microaerophiles

Frage 9

Frage
Which of the following is an indirect measurement of bacterial growth?
Antworten
  • Flow cytometry
  • Viable counting
  • Petroff-Hauser cell counting
  • Optical density

Frage 10

Frage
Select the correct order in a bacterial growth curve:
Antworten
  • Log phase --> stationary phase --> lag phase --> death phase
  • Log phase --> lag phase --> death phase -->stationary phase
  • lag phase --> log phase --> death phase -->stationary phase
  • lag phase --> log phase -->stationary phase -->death phase

Frage 11

Frage
Which of the following is the correct definition for the thermal death point?
Antworten
  • Minimal temperature at which all organisms are killed in 10 min in a particular liquid
  • Minimal temperature at which all organisms are killed in 1 hour in a particular liquid
  • Minimum time taken to kill all organisms at particular temperature in a given liquid
  • Minimal temperature at which all organisms are killed in 10 min in water

Frage 12

Frage
Which temperature does the dry heat (oven) method of steralisation occur at?
Antworten
  • 121 degrees celcius
  • 150 degrees celcius
  • 70 degrees celcius
  • 100 degrees celcius

Frage 13

Frage
Which of the following ways does non-ionising radiation cause damage to the cell?
Antworten
  • By breaking the DNA into fragments
  • By creating reactive oxygen species
  • By lysing the cell membranes
  • By mutating the DNA

Frage 14

Frage
Which of the following states would filtration be an appropriate method of sterilisation?
Antworten
  • Gas
  • Liquid
  • Solid

Frage 15

Frage
Which of the following shows a bactericidal treatment? (note: top line shows cell count and bottom line shows viable cell count, x axis= Log number of cells y axis = Time)

Frage 16

Frage
Which of the following kills microorganisms but not necessarily endospores?
Antworten
  • Disinfectants
  • Sterilants
  • Antiseptics

Frage 17

Frage
Which of the following can be applied to living tissue?
Antworten
  • Germicides
  • Antiseptics
  • Disinfectants
  • Sterilants

Frage 18

Frage
Which of the following is a disc diffusion technique that allows the effective concentration to be seen?
Antworten
  • E-test
  • Minimum inhibitory concentration
  • Minimum bactericidal concentration

Frage 19

Frage
What is the minimum bactericidal concentration?
Antworten
  • The lowest concentration of a drug killing >99.9% of a test organism after overnight incubation.
  • The lowest concentration of a drug inhibiting the visible growth of a test organism after overnight incubation.
  • The lowest concentration of a drug killing >99.9% of a test organism after 48 hours of incubation.
  • The highest concentration of a drug killing >99.9% of a test organism after 48 hours of incubation.

Frage 20

Frage
Which of the following does not cause cell death by interacting with proteins?
Antworten
  • Phenolic compounds
  • Alcohols
  • Aldehydes
  • Quaternary ammonium compounds
  • Halogen releasing agents

Frage 21

Frage
Which are the two main classes of halogen releasing agents used in microbial control?
Antworten
  • Iodine-releasing
  • Chlorine-releasing
  • Bromine-releasing
  • Fluorine-releasing

Frage 22

Frage
What is the active concentration of alcohol? (at which it disrupted cell components)
Antworten
  • 40-60%
  • 60-80%
  • 80-100%
  • 20-40%

Frage 23

Frage
Which of the following is true of phenolic compounds?
Antworten
  • They often stain
  • They are toxic at high concentrations
  • They act as a local anesthetic at low concentrations
  • They form chlorinated bases in DNA

Frage 24

Frage
Who formally demonstrated the theory of germs?
Antworten
  • Louis Pasteur
  • Robert Koch
  • Rudolf Emmerich and Oscar Loew
  • Paul Ehrlich

Frage 25

Frage
Which of the following are not part of Koch's postulates on the causal relationship between microorganisms and disease.
Antworten
  • The microorganism must be found in all organisms suffering from the disease, and in healthy organisms.
  • The microorganism must be re isolated from the inoculated, diseased experimental host and identified as being identical to the original specific causative agent.
  • The cultured microorganism should cause disease when introduced into an immunocompromised organism.
  • The microorganism must be isolated from a diseased organism and grown in pure culture.

Frage 26

Frage
Who won the nobel prize for the showing prontosil red had antibacterial properties?
Antworten
  • Rudolph Emmerich & Oscar Loew
  • Paul Ehrlich
  • Alexander Fleming
  • Gerhard Domagk

Frage 27

Frage
Which cell process does penicillin inhibit?
Antworten
  • Protein synthesis
  • DNA gyrase
  • Cell wall synthesis
  • Folic acid metabolism

Frage 28

Frage
How many patients were affected by nosocomial infections in Europe in 2011?
Antworten
  • 14 billion
  • 14 million
  • 1.7 billion
  • 1.7 million

Frage 29

Frage
Which of the following is a cause of antibiotic resistance?
Antworten
  • Farming industry
  • Pets
  • Agriculture
  • Aquaculture
  • Human misuse

Frage 30

Frage
Which of the following is not a property of an ideal antibiotic?
Antworten
  • Non-toxic/inert
  • Stable
  • Cheap (relatively)
  • selectively distrubuted

Frage 31

Frage
How do D,D-transpeptidases act in peptidoglycan cell wall synthesis?
Antworten
  • Covalently bind murNAc and glcNAc together
  • Catalyses the formation of cross-links between D-ala side chains
  • Inhibit peptide binding proteins
  • Bind to the beta-lactam ring of beta-lactam antibiotics

Frage 32

Frage
Overproduction of MexAB-OprM gives imipenem resistance.
Antworten
  • True
  • False

Frage 33

Frage
Which of the following is not a way that beta-lactam resistance can form?
Antworten
  • Change in Pencillin Binding protein pathway
  • Modification of beta-lactam molecules
  • The binding of beta-lactamases to beta lactams using a catalytic serine
  • Mutation of PBPs
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