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Beschreibung
Quiz von Alexandra McCabe, aktualisiert more than 1 year ago
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Erstellt von Alexandra McCabe
vor etwa 9 Jahre
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Frage 1
Frage
What is the best way to assemble a genome?
Antworten
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Have a reference gene to different species
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Have a reference gene to same species
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Have a reference genome to same species
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Have a reference genome to different species
Frage 2
Frage
Which is better DNA plat forms?
Antworten
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Shorter reads cause of lower accurancy
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Longer reads easier to assemble
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Shorter reads cause of higher accurancy
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Longer reads harded to assemble
Frage 3
Frage
What happens next after you have fragments?
Antworten
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Place contigs together to see where they fit on the reference genome
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Place contigs together next to eachother
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Analyse each contig
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Place contigs far apart
Frage 4
Frage
How do you increase the coverage for genome sequencing?
Antworten
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Decrease the number of sequencing
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Increase the number of sequencing
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Use more DNA
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Use reference DNA
Frage 5
Frage
Sequence gap:
Antworten
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Don't have enough sequence but know the order and orientation of the contigs
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No information about the adjacent gap or spanning gap
Frage 6
Frage
Physical Gaps:
Antworten
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No information about the adjacent gap or spanning gap
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Know the order and orientation of the contigs but dont have enough sequence
Frage 7
Frage
What is the function of genome scaffolds?
Antworten
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Rearrange the genome
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Rearrange gene
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Construct and arrange contigs in relation to one another
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Place contigs on a stiff sufface
Frage 8
Frage
What step is after contig production when you are trying to build a gemone?
Antworten
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Genome scaffolds with gaps in between contigs
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Join contigs using evidence from pair end data
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Place the contigs right next to eachother with no gaps
Frage 9
Frage
Why would you no fill the gap that is found between contigs on the genome scaffold?
Antworten
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A customer might only want a scaffold of the genome
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Gaps can be over looked
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Customer might want to fill it in himself
Frage 10
Frage
What does N50 mean?
Antworten
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The value of which 50% of nucleotides sequences are represented
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The percentage of gaps in the scaffold
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The number of genes produced
Frage 11
Frage
A k-mer are nucleotides that have been cut down to a certain size
Antworten
- True
- False
Frage 12
Frage
What is the function of a contig?
Antworten
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Represent a consensus region of DNA
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Represent the genome
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Represent the gene
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Represent a consensus region of RNA
Frage 13
Frage
How can you guess gap sizes on scaffold?
Antworten
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Incorporating info from pair ends of the contigs
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Randomly guess
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Place all the contigs beneath each other
Frage 14
Frage
Long insert library
Antworten
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4-40 kb library that has 100 bp on each end
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4-8 kb library that has 100 bp on each end
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4-40 kb library that has 10 bp on each end
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4-8 kb library that has 10 bp on each end
Frage 15
Frage
Why is De Novo genome assembly useful?
Antworten
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Suitable for short coverage and long reads
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Suitable for long coverage and long reads
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Suitable for long coverage and short reads
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Suitable for short coverage and short reads
Frage 16
Frage
Why is the De Bruijn Graph useful?
Antworten
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Suitable for high coverage and long reads
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Suitable for high coverage and short reads
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Suitable for short coverage and long reads
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Suitable for short coverage and short reads
Frage 17
Frage
How long do the k-mers need to be in overlap-layout-consensus?
Antworten
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approx 24
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approx 2
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approx 240
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approx 2400
Frage 18
Frage
Why do the k mers in the De Bruijn model need to be similar sizes?
Antworten
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If they are too small, it won't show overlaps
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If they are too long, it won't show overlaps
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If they are too small is easier to sequence
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If they are too long is easier to sequence
Frage 19
Frage
What is problem that arises when assembling fragments?
Antworten
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Repeats
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mRNA
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cDNA
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gDNA
Frage 20
Frage
How do you solve the problem of repeats in genome assembly?
Antworten
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Increase reading length which decreases sequence error rate
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Increase reading length which increases sequence error rate
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Decrease reading length which decreases sequence error rate
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Decrease reading length which increases sequence error rate
Frage 21
Frage
What takes up 45% of the genome?
Antworten
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Large duplications
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Simple repeats
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Introns and Exons
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Transposons
Frage 22
Frage
Intergenic DNA have parasitic DNA that often fail to replicate properly
Antworten
- True
- False
Frage 23
Frage
What is the name of the process whereby an exon from Gene A is placed in Gene B?
Antworten
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Exon duplication
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Exon Shuffling
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Intron Duplication
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Intron Shuffling
Frage 24
Frage
What is the process whereby an exon is repeated and forms a new gene variant?
Antworten
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Exon Duplication
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Exon Shuffling
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Intron Duplication
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Intron Shuffling
Frage 25
Frage
Entire gene families can be duplicated
Antworten
- True
- False
Frage 26
Frage
What happens when entire gene super families get duplicated?
Antworten
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Can double the number of copies of every gene and gene family
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Can half the number of copies of every gene and gene family
Frage 27
Frage
What happens when misaligned chromatids
Antworten
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Repeats
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Transposons
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Exons
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Introns
Frage 28
Frage
Homologous genes in different organisms that encode proteins with the same function and have evolved by direct vertical descent
Antworten
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Orthologous Genes
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Paralogous Genes
Frage 29
Frage
Homologous genes within an organism encoding proteins with related functions and often arise due to gene duplication
Antworten
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Orthologous genes
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Paralogous genes
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