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Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor fast 11 Jahre
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Frage 1
Frage
DNA helicase unwinds DNA into ssDNA for what processes?
Antworten
-
DNA repair
-
DNA replication
-
DNA recombination
-
DNA destruction
Frage 2
Frage
DNA helicase's process and translocate branched structures
Antworten
- True
- False
Frage 3
Frage
Name (a) branched DNA structure(s)
Antworten
-
Holliday junctions
-
D loops
-
Binary helix
-
T loops
Frage 4
Frage
RNA helicases cannot destabilize RNA secondary structures
Antworten
- True
- False
Frage 5
Frage
RNA helicases help in which of the following processes
A- Promote ribosome assembly
B- RNA translocation
C-RNA splicing
D- RNA editing and transport
E-RNA degredation
Antworten
-
A, C & D
-
B, C & E
-
A only
-
E only
-
All of them
Frage 6
Frage
Some helicases can work on both RNA & DNA
Antworten
- True
- False
Frage 7
Frage
Which of the following unwind RNA-DNA hybrids
Antworten
-
RNA helicase, then DNA helicase
-
DNA helicase, then RNA helicase
-
RNA/DNA helicases
Frage 8
Frage
RNA/DNA helicases perform which of the following functions?
Antworten
-
Transcription termination
-
Regulation of DNA replication initiation
-
RNA degredation
Frage 9
Frage
All helicases use energy from NTP hydrolysis to translocate along polynucleotides
Antworten
- True
- False
Frage 10
Frage
Helicases are dependent on Fe2+
Antworten
- True
- False
Frage 11
Frage
Helicases can take which of the following forms (commonly)
Antworten
-
Hexamers
-
Monomers
-
Trimers
-
Dimers
-
Tetramers
-
Pentamers
Frage 12
Frage
Helicases can have an oligomeric structure
Antworten
- True
- False
Frage 13
Frage
Helicases can be
Antworten
-
Unidirectional
-
Bidirectional
-
Both
-
Bidirectional or unidirectional
Frage 14
Frage
What is the difference between translocases and helicases?
Antworten
-
Translocases -transcribe and separate DNA strands Helicases- transcribed DNA strands only
-
Helicases-transcribe and separate DNA strands Translocases- transcribed DNA strands only
Frage 15
Frage
Give an example of a DNA/RNA unwinding helicase/translocase
Antworten
-
PCRA
-
Type 1 restriction enzymes
-
Ruv B/Reg G
-
Mfd
Frage 16
Frage
Name a helicase/translocase that effects protein motion on DNA
Antworten
-
PCRA
-
Type 1 Restriction Enzyme
-
Ruv B/ Reg G
-
Mfd
Frage 17
Frage
Name a necleoprotein complex remodelling helicase
Antworten
-
Mfd
-
Ruv B/Reg G
-
PCRA
-
Type 1 Restriction Enzymes
Frage 18
Frage
Name a DNA/RNA remodelling helicase
Antworten
-
Ruv B/ Reg G
-
PCRA
-
Type 1 Restriction Enzymes
-
Mfd
Frage 19
Frage
Helicases are divided into seven superfamilies based on primary structure, and then 3 structural classes
Antworten
- True
- False
Frage 20
Frage
The two structural classes are monomers and dimers
Antworten
- True
- False
Frage 21
Frage
All helicases contain a Rec B fold
Antworten
- True
- False
Frage 22
Frage
EVERY helicase molecule contains which of the following?
Antworten
-
An ATP binding site
-
An NTP binding site
-
A separate polynucleotide binding site
-
A GTP binding site
-
A linked polynucleotide binding site
Frage 23
Frage
The _TP binding site and the nucleotide binding site on a helicase are allosterically linked
Antworten
- True
- False
Frage 24
Frage
Type A helicases (like PCRA) travel in which direction?
Antworten
-
3' > 5'
-
5' > 3'
-
Both ways
Frage 25
Frage
Rec D is a Type 2 helicase- in which direction does it travel?
Antworten
-
5' > 3'
-
Both ways
-
3' . 5'
Frage 26
Frage
Rec BCD is a bipolar helicase
Antworten
- True
- False
Frage 27
Frage
In a bacterial Holliday Junction with a stalled replication fork, what is required to allow replication to continue?
Antworten
-
RuvB
-
ATP
-
PRCA
-
GTP
Frage 28
Frage
If Holliday Junctions cannot be repaired, they lead to a cruciform structure
Antworten
- True
- False
Frage 29
Frage
What is the slowest rate of movement of a helicase?
Antworten
-
10 bp/sec
-
8 bp/sec
-
12 bp/sec
Frage 30
Frage
Rate of helicase movement is dramatically dependent on protein partners
Antworten
- True
- False
Frage 31
Frage
The fastest known helicase moves around 4000 bp/sec
Antworten
- True
- False
Frage 32
Frage
Define Helicase Processivity
Antworten
-
How fast it moves along the DNA/RNA
-
How far it can move before it falls off
-
How far it moves per substrate hydrolysis
Frage 33
Frage
Helicases can move around 10-30,000 base pairs before falling off depending on the helicase (and it's protein partners)
Antworten
- True
- False
Frage 34
Frage
Step size is a theoretical limit due to the entropy of NTP hydrolysis
Antworten
- True
- False
Frage 35
Frage
Step size is the theoretical avg no of bp unwound per NTP hydrolysis cycle
Antworten
- True
- False
Frage 36
Frage
The limit on step size = Energy required to melt the bp x step size
Antworten
- True
- False
Frage 37
Frage
What is the experimental limit of step size?
Antworten
-
0.3-3 bp
-
0.5- 5bp
-
0.7- 7 bp
Frage 38
Antworten
-
The 'Inchworm' helicase movement model
-
The 'active rolling model'
Frage 39
Frage
Which of the following is believed to be the unwinding mechanism used by helicases?
Antworten
-
Passive- DNA is frayed by heat and the translocation occurs in the presence of ATP
-
Active- ATP activates molecules that bind to and actively distort DNA before ATP dependent translocation can occur
Frage 40
Frage
PCRA is made of how many domains?
Antworten
-
4 - 1A/B & 2A/B
-
2 - 1A & 2A
-
6 1A/B, 2A/B & 3A/B
Frage 41
Frage
PCRA activity is dependent on its protein partners
Antworten
- True
- False
Frage 42
Frage
PCRA is an ATPase with...
Antworten
-
A 3' > 5' polarity
-
A 5' > 3' polarity
-
A low rate of 30 bp/sec
-
A high rate of 300 bp/sec
-
A low processivity of 10 bp/sec
-
A high processivity of 100 bp/sec
-
A step size of 16 bp/ATP
-
A step size of 6 bp/ATP
Frage 43
Frage
The conserved Helicase motifs of PCRA are in the accessory domains
Antworten
- True
- False
Frage 44
Frage
Aromatic stacking occurs between the inserted aa and bp
Antworten
- True
- False
Frage 45
Frage
Which of the following will bind to the DNA
Antworten
-
Tryptophan
-
Phenylalanine
-
Tyrosine
-
Histidine
Frage 46
Frage
The complex of PCRA-DNA remains the same during ATP binding and ATP hydrolysis
Antworten
- True
- False
Frage 47
Frage
Duplex distortion in the PCRA substrate complex suggests a Passive unwinding system
Antworten
- True
- False
Frage 48
Frage
Partner proteins are required by PCRA for which processes?
Antworten
-
Loading
-
Unwinding
-
Activation
-
Catalytic modification
Frage 49
Frage
Hexameric helicases can be part of the replisome
Antworten
- True
- False
Frage 50
Frage
Hexameric helicases have a toroidal strcture to confer high processivity
Antworten
- True
- False
Frage 51
Frage
ATP binds where on hexameric helicases?
Antworten
-
To the pockets present in each monomer
-
To the pocket made by the interaction of all 6 monomers
Frage 52
Frage
ssDNA is bound in the central channel to a 'spiral staircase' of bining loops
Antworten
- True
- False
Frage 53
Frage
ATP binding pocket changes _ around the ring
Antworten
-
Sequentially- one at a time
-
At the same time
-
Sequentially- two at a time
Frage 54
Frage
ATP binding correlates with loop hight in the central channel
Antworten
- True
- False
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