453902
Beschreibung
Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor fast 11 Jahre
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Frage 1
Frage
What is required for the protein surface to bind to the DNA surface?
Antworten
-
For the protein surface to be complimentary to the DNA surface
-
For the protein surface to be positive (DNA backbone's are negative)
-
For the protein surface to contain hydrophobic residues
Frage 2
Frage
In which groove can the order of base pairs be differentiated?
Antworten
-
Major groove
-
Minor groove
Frage 3
Frage
The major groove is 15A wide
Antworten
- True
- False
Frage 4
Frage
Which is smaller?
Antworten
-
Major groove
-
Minor groove
Frage 5
Frage
Proteins that bind in the major groove must be more specific than those that bind in the minor groove
Antworten
- True
- False
Frage 6
Frage
Why is Arg such a useful residue?
Antworten
-
It has 2 H acceptors, so it can bind to guanine
-
It has 2 H donors, so it can bind to guanine
-
It has 2 H acceptors, so it can bind to cytosine
Frage 7
Frage
Which of the following can bind to both G & A
Antworten
-
Glu
-
Val
-
Asn
-
Tyr
Frage 8
Frage
What is base stacking?
Antworten
-
Where hydrophobic bases stack to avoid water
-
Where hydrophobic bases pull the DNA inwards to avoid water
-
Where hydrophobic bases twist the DNA to avoid water
Frage 9
Frage
What is required for a protein to take advantage of base stacking?
Antworten
-
Residues with delocalised electrons
-
Hydrophobic residues
-
Hydrphillic residues
-
For it to make a gap between bases
Frage 10
Frage
10bp covers a distance of 34 A
Antworten
- True
- False
Frage 11
Frage
Describe DNA
Antworten
-
Long, repetitious and thin
-
Long, non-repetitious and thick
-
Short, repetitious and thin
-
Long, repetitious and thick
-
Short, non-repetitious and thin
Frage 12
Frage
DNA can bend 180 dgrees over how long a stretch?
Antworten
-
40
-
30
-
20
Frage 13
Frage
What does Phage Lamda Repressor bind to?
Antworten
-
Phage DNA
-
Bacterial DNA
Frage 14
Antworten
-
A half site
-
A palindromic sequence
-
A restriction site
Frage 15
Frage
How does Phage Lambda Repressor Work?
Antworten
-
Binds to bacterial DNA at the lambda cro gene
-
Binds to phage DNA at the Lambda cro gene
-
And blocking RNA polymerase binding
Frage 16
Frage
Phage Lambda Repressor binds to only one target sequence
Antworten
- True
- False
Frage 17
Frage
The Phage Lambda repressor binds as a _
Antworten
-
Monomer
-
Dimer
-
Tetramer
Frage 18
Frage
Each subunit attaches to the DNA using a _ and binds to the other subunits using a _
Antworten
-
Alpha helix, alpha helix
-
Alpha helix, nothing- it's a monomer
-
Beta sheet, beta sheet
-
Beta sheet, nothing- it's a monomer
Frage 19
Frage
Phage Lambda Repressor has multiple subunits because ...
Antworten
-
It binds to a sequence long enough that the protein cannot be removed
-
It binds to a sequence long enough that the sequence becomes unique int the DNA
-
It binds more exactly - so it only has a single target sequence
Frage 20
Frage
Where does Phage Lambda Suppressor bind?
Antworten
-
2 adjacent major grooves
-
2 adjactent minor grooves
-
An adjactent major & minor groove
-
32A apart
-
34A apart
-
36A apart
Frage 21
Frage
A non-specific interaction doesn't involve bonding with any particular base
Antworten
- True
- False
Frage 22
Frage
List some specific interactions between Phage Lambda Repressor and DNA
Antworten
-
Ser - G, and Gly - T,
-
Gly- T and Ser-G
-
Gly- C- and Ser-T
Frage 23
Frage
The DNA sequence is all read from the same DNA strand
Antworten
- True
- False
Frage 24
Frage
A helix-turn-helix motif is often used for reading DNA
Antworten
- True
- False
Frage 25
Frage
Different alpha helices (of the helix-turn-helix) motif perform specific and non-specific interactions
Antworten
- True
- False
Frage 26
Frage
Proteins don't have to land on the DNA in exactly the right spot, they only need to...
Antworten
-
Bind to the phosphate backbone
-
Bind to the bases
-
And then roll freely along the DNA
Frage 27
Frage
Methionine repressor...
Antworten
-
prevents methionine production
-
prevents binding of methionine to tRNA
-
prevents methionine being added to the amino acid chain
Frage 28
Frage
The Methionine Repressor binding site is...
Antworten
-
Palindromic
-
Almost palindromic
-
8bp
-
16bp
Frage 29
Frage
The binding site of most helix-turn-helix proteins are 16-18bp long
Antworten
- True
- False
Frage 30
Frage
How does the Methionine repressor bind to the DNA?
Antworten
-
Uses 2 alpha helices
-
Uses 2 beta sheets
-
One from each monomer
-
One from each end of the monomer
-
Into one major groove
-
Into two major grooves
Frage 31
Frage
Two beta strands have the same diameter as an alpha helix
Antworten
- True
- False
Frage 32
Frage
Specific interactions in the methionine repressor happen between Lysine, Threonine, A & G
Antworten
- True
- False
Frage 33
Frage
Restrictions enzymes are part of the bacterial immunity... how?
Antworten
-
Recognises and destroys non-self DNA
-
Cuts around viral DNA that has infected the genome
-
Proteases break down the viral coat
Frage 34
Frage
Restriction enzymes and transferases come in pairs, with the restriction enzyme made first
Antworten
- True
- False
Frage 35
Frage
How does EcoRI bind?
Antworten
-
Phosphate backbone reactions in the minor groove
-
Phosphate backbone reactions in the major groove
-
Specific interactions in the minor groove
-
Specific interactions in the major groover
Frage 36
Frage
EcoRI looks for 2 G-C on top of each other because they're more bendable
Antworten
- True
- False
Frage 37
Frage
Where in the GAATTC sequence does EcoR1 cut?
Antworten
-
After the C, before the C
-
After the A & T
-
In the middle
Frage 38
Frage
Eco RI needs an Fe iron to bind
Antworten
- True
- False
Frage 39
Frage
Where are the distortions of the DNA caused by EcoRI?
Antworten
-
In the centre of GAATTC
-
G/AATT/C
-
GA/AT/TC
Frage 40
Frage
Hydrophobic interactions in ECORI-DNA ineractions recognise what?
Antworten
-
AA atacking
-
AT stacking
-
TT stacking
Frage 41
Frage
ECORI uses water mediated interactions to assist DNA recognition
Antworten
- True
- False
Frage 42
Frage
What is strange about the EcoRV restriction enzyme?
Antworten
-
It has an abnormally large binding site
-
It leaves blunt ends
-
It binds to 2 different sequences
Frage 43
Frage
EcoRV forms a dimer
Antworten
- True
- False
Frage 44
Frage
ECORV creates major distortions where?
Antworten
-
In the middle
-
At the ends
-
One residue in
Frage 45
Frage
ECORV binds to the backbone in the minor groove and wraps 'arms' around into the major groove
Antworten
- True
- False
Frage 46
Frage
The recognition loops in the major groove cause...
Antworten
-
H bonding between stacks
-
H bonding between A-G
-
H bonding between T-C
Frage 47
Frage
ECORV looks for ATAT bases
Antworten
- True
- False
Frage 48
Frage
Where in the GCGC sequence does M.Hal methylate?
Antworten
-
The first G
-
The first C
-
The second G
-
The last C
Frage 49
Frage
Methylation prevents the restriction enzyme from recognizing a sequence, so it cannot be cot
Antworten
- True
- False
Frage 50
Frage
Describe the actions of methylases
Antworten
-
Methylate both strands at once
-
Methylate one strand at a time
-
Are monomers
-
Are dimers
Frage 51
Frage
Interactions 'pull out' one of the bases to gain chemical access
Antworten
- True
- False
Frage 52
Frage
What is the donor for methylation?
Antworten
-
S-adenosyl-methionine
-
Methanol
-
Methide
Frage 53
Frage
How the methylation interaction studied?
Antworten
-
The cystine had the 5th H replaced with an F
-
Trapping it in the active site
-
The cystine had the 3rd H replaced with an F
Frage 54
Frage
Once in the active site, a thiol bond forms between the cystine and the cytosine so the methyl can be added
Antworten
- True
- False
Frage 55
Frage
What is inserted to bind with the widowed G?
Antworten
-
Glu
-
Arg
-
Cys
Frage 56
Frage
The inserted bases fill up the space left by the flipped out cytosine
Antworten
- True
- False
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