5394622
Beschreibung
Quiz von Sam Watkins, aktualisiert more than 1 year ago
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Erstellt von Sam Watkins
vor etwa 8 Jahre
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Frage 1
Frage
In specimen collection the plant must be dried and kept sterile to minimise damage
Antworten
- True
- False
Frage 2
Frage
Which of the following is best to aid identification of the natural product/plant
Antworten
-
plant handbook
-
flora or field guide
-
herbarium
Frage 3
Frage
macerated leaves are treated with chemicals that gradually decrease in polarity to extract chemicals
Antworten
- True
- False
Frage 4
Frage
General screening bioassays are [blank_start]simple[blank_end], [blank_start]inexpensive[blank_end] and used for [blank_start]rapid screening[blank_end] of extracts
Antworten
-
simple
-
complex
-
inexpensive
-
expensive
-
rapid screening
-
specific enzyme screening
Frage 5
Frage
specialised screening bioassays are [blank_start]subcellular[blank_end] assays directed at [blank_start]specific[blank_end] enzymes or receptors. Compounds working by mechanisms unrelated to the assay are likely to be missed.
Antworten
-
subcellular
-
multicellular
-
specific
-
broad spectrum
Frage 6
Frage
which of the following is FALSE
Antworten
-
Brine shrimp lethality tests are used to determine cytotoxicity (potential antitumour) of extracts
-
crown gall tumour inhibition tests give a fairly accurate prediction of in vivo murine antileukemic activity
-
starfish or sea urchin assays are used to investigate in vivo antileukemic activity
-
agar dilution streak assay means up to 7 microorganisms can be screened simultaneously on a petri dish
-
microtitre plates allow testing of 3 dilutions of one or two extracts against up to 24 microorganisms
Frage 7
Frage
crown gall tumour inhibition tests use potatoes to measure inhibition of growth
Antworten
- True
- False
Frage 8
Frage
which 3 do starfish or sea urchin assays allow detection of?
Antworten
-
dna and rna synthesis inhibitors
-
mRNA substrate inhibitors
-
microtubule assembly inhibitors
-
protein synthesis inhibitors
Frage 9
Frage
which best applies to an agar dilution streak assay?
Antworten
-
up to 5 micro-organisms can be tested
-
up to 4 micro-organisms can be tested
-
up to 7 micro-organisms can be tested
Frage 10
Frage
Which tlc staining technique is used to test acidic groups
Antworten
-
bromocrescol green
-
vanillin
-
potassium permanganate
Frage 11
Frage
which one of the following is not considered in NP isolation or synthesis
Antworten
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source - availability
-
environmental and local impact
-
cost
-
complexity of novel structures
-
diluent needed
Frage 12
Frage
What are the stages of natural product processing? [blank_start]Specimen[blank_end] collection, establishing [blank_start]taxonomy[blank_end], extracting possible active molecules, using [blank_start]screening[blank_end] techniques to evaluate therapeutic activity, identifying and [blank_start]isolating[blank_end] the structure responsible for the activity, using organic synthesis
Antworten
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Specimen
-
Taxonomy
-
molecules
-
structure
-
taxonomy
-
specimen
-
molecules
-
therapeutics
-
screening
-
showing
-
therapeutic
-
herbariums
-
isolating
-
burning
-
melting
-
releasing
Frage 13
Frage
a range of techniques are used to isolate active compounds (fractionation). these include [blank_start]TLC[blank_end], column chromatography, [blank_start]HPLC[blank_end] and combinations such as HPLC/MS
Antworten
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TLC
-
LTC
-
MSMS
-
HPLC
-
ELISA
-
SPE
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