Prokaryotic Transcription & Translation

Initiation of transcription The [blank_start]core RNA polymerase[blank_end] binds to the [blank_start]sigma factor[blank_end] to produce the RNA polymerase [blank_start]holoenzyme[blank_end]. Then this binds to the [blank_start]promoter[blank_end]. Then a transcription bubble is formed. Next there is the creation of bonds between rNTPs. Finally there is an escape from transcription apparatus from promoter.

Initiation of translation [blank_start]Small[blank_end] subunit binds to [blank_start]Shine-Dalgarno[blank_end] sequence with the help of [blank_start]IF3[blank_end] Then [blank_start]fMet-tRNA[blank_end] binds to initiation codon with the help of IF1, IF2 and [blank_start]GTP[blank_end] This is called the [blank_start]30s[blank_end] initiation complex Then IFs are removed and [blank_start]large[blank_end] subunit is added This is called the [blank_start]70s[blank_end] initiation complex In summary the ribosome has been assembled on the mRNA and the first tRNA is attached to the initiation codon