Factors Affecting Enzyme Activity

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A level Biology (2.1.4 Enzymes) Karteikarten am Factors Affecting Enzyme Activity, erstellt von Yinka F am 26/01/2018.
Yinka F
Karteikarten von Yinka F, aktualisiert more than 1 year ago
Yinka F
Erstellt von Yinka F vor fast 7 Jahre
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Zusammenfassung der Ressource

Frage Antworten
What factors affect enzyme activity? Temperature pH Enzyme concentration Substrate concentration
How does temperature affect enzyme activity? More heat = more kinetic energy Molecules move faster, increasing the frequency of successful collisions (substrates are more likely to collide with active sites) Every enzyme has an optimum temperature
What happens if the temperature gets too high? The enzyme's bonds may break, changing the shape of the active site and the enzyme denatures
How does pH affect enzyme activity? Above and below the optimum pH, the H⁺ and OH⁻ ions can break the ionic and hydrogen bonds that hold the enzyme's tertiary structure in place. This makes the active site change shape and the enzyme denatures
How does enzyme concentration affect enzyme activity? The more enzyme molecules, the more likely a substrate is to collide, forming enzyme-substrate complexes
What happens if the substrate concentration is a limiting factor? There comes a point when there are more than enough enzymes, so adding more enzymes has no further effect
How does substrate concentration affect enzyme activity? The frequency of successful collisions increases, so more active sites are occupied and more enzyme-substrate complexes will form, until a saturation point
What happens when enzyme concentration becomes a limiting factor? There are not enough active sites as they are all full
What happens to substrate concentration over time and how does this affect enzyme activity? Substrate concentration decreases with time, and so the rate of reaction will decrease over time too. Therefore, the initial rate of reaction is the highest rate of reaction
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