Secondary (invivo) screening

Beschreibung

The invivo preparations and the tests carried on them
Ayman Barghout
Karteikarten von Ayman Barghout, aktualisiert more than 1 year ago
Ayman Barghout
Erstellt von Ayman Barghout vor mehr als 8 Jahre
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In-vivo preparations 1. Anesthetized cat blood pressure. 2. Cat nictitating membrane
Anesthetized cat blood pressure preparation Animals are cannulated at 3 sites: 1. Tracheal cannulation. 2. Femoral vein cannulation. 3. Femoral artery cannulation
1. Tracheal cannulation Polyethylene cannula is introduced into the trachea, cannula is connected to respirometer (artificial ventilator) to overcome respiratory pralysis produced by skeletal muscles relaxants.
2. Femoral vein cannulation Through a small cut in the vein connected to a syringe for IV adminstration of drugs
3. Femoral artery cannulation The femoral artery is connected to a blood pressure transducer for blood pressure measurement which converts blood pressure to electronic signals which are recorded on a multi-channel system
Tetrad system 1. Electrical vagal stimulation. 2. IV administration of acetylcholine. 3. Bilateral Carotid Occlusion (OCB). 4. IV administration of epinephrine or norepinephrine.
Vagus nerve The parasympathetic nerve supplying the heart & GIT.
Vagus nerve origin It originates from 2 nuclei in the meddula oblongata 1. Dorsal motor nucleus of vagus (DMV) 2. Nucleus ambiguous (NA)
1. Electrical vagal stimulation 1. The neck area is opened and one of the two vagi is dissected. 2. Vagus nerve is stimulated electrically (preganglionic stimulation)
Effect of electrical vagal stimulation 1. Release of endogenous Acetylcholine which affects \[M_2\] receptor in heart. 2. Bradycardia → decreased cardiac output → hypotension.
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