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27049056
Extracción de flavonoles de semillas
Description
flavonoles
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flavonoles
grado superior
Flowchart by
luis temiño
, updated more than 1 year ago
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Created by
luis temiño
over 3 years ago
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Resource summary
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INICIO
Pulverizar 100mg de tejido
Añadir 150 uL de acetonitrilo 75%
Vórtex
Sonicar 10 minutos
Centrifugar a 14000 rpm durante 5 minutos
Pasar el sobrenadante a un vial nuevo con cierre hermético
2ª extracción
Añadir 300 uL de HCl 2N
Incubar 40 minutos a 70ºC
Dar un spin
Añadir 300 uL de actonitrilo al 75%
Centrifugar 5 minutos a 14000 rpm
Pasar a vial UPLC
FIN
Volvemos a este paso
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