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33927025
Diagrama preparación de tinción de Ziehl-Neelsen
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Flowchart on Diagrama preparación de tinción de Ziehl-Neelsen , created by Luna Oliveros Mariana on 14/10/2021.
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Luna Oliveros Mariana
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Luna Oliveros Mariana
about 3 years ago
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Proceso para elaboración de tinción de Ziehl-Neelsen
1-Preparar un frotis bacteriano en un portaobjetos y fijarlo
2-Cubrir la preparación con carbofucsina
3- Calentar el portaobjetos sin que hierva por 5-10 minutos
4- Enjuagar con agua el exceso de colorante
5- Decolorar con una solución de ácido-alcohol por 30 segundos
6- Enjuagar con agua el exceso
7- Agregar azul de metileno por 1 minuto
8- Lavar el exceso de colorante con agua
9- Secar la preparación
Fin
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