Structures and Functions in Living Organisms

2.1 Level of organisation

Organelle (e.g. cytoplasm): subcellular structures found within living cells
Cell (e.g. muscle cell / palisade cell): basic structural unit of a living organism
Tissue (e.g. muscle tissue / palisade tissue): group of similar cells working together to carry out a particular function
Organ (e.g. stomach / leaf): group of different tissues working together to carry out a particular function
Organ system (e.g. digestive system / shoot system): group of organs working together to carry out a particular function
Organism (e.g. human / sunflower): a form of life

2.2-2.6 Cell Structure

Nucleus: stores genetic material
Cytoplasm - where chemical reactions take place
Cell membrane: controls which substances can pass in and out of the cell
Mitochondria: provides energy for the cell through respiration
Ribosomes: site of protein synthesis
Rigid cell wall (plants): provides support and structure
Chloroplasts (plants): carry out photosynthesis with chlorophyll absorbing light from the sun
Permanent vacuole (plants): contains cell sap (sugars, salts, water)

2.7-2.14 Biological molecules

Carbohydrates: carbon, hydrogen, oxygen, starch and glycogen from simple sugars (e.g. glucose, fructose)
Proteins: carbon, hydrogen, oxygen, nitrogen, proteins from amino acids
Lipids: carbon, oxygen, hydrogen, lipids from 3 fatty acids + glycerol
Glucose test: benedict's solution + water bath, blue to brick-red
Starch test: iodine, brown to blue-black
Protein test: biuret A + biuret B, blue to purple
Fat test: ethanol + distilled water, colourless to milky white emulsion
Enzyme: biological catalysts which increase the rate of reaction
Enzymes + temperature: rate increases as temperature increases (particles have more kinetic energy so move faster and higher chances of collision), optimum 37
Enzymes + high temperature: bonds are broken, active site changes shape, enzyme cannot bind with the substrate, enzyme is denatured
Enzyme + temperature exp: heat starch to set temperature, add amylase, add iodine to each well after a minute, measure time till iodine stops turning blue-black
Enzyme + temperature exp: independent - temperature, dependent - rate of starch breakdown, control - volume of starch, concentration of starch
Enzymes + pH: rate increases as neutrality increases (particles have more kinetic energy so move faster and higher chances of collision), optimum 7
Enzymes + high pH: bonds are broken, active site changes shape, enzyme cannot bind with the substrate, enzyme is denatured
Enzyme + pH exp: heat starch + amylase + pH solution, add a drop of the solution to each well with iodine after 10 seconds, measure time till solution turns orange
Enzyme + pH exp: independent - pH, dependent - rate of starch breakdown, control - temperature, volume of starch, concentration of starch

2.15-2.17 Movement of substances into and out of cells

Diffusion: the net movement of particles from an area of high concentration to an area of low concentration
Diffusion example: single-celled organisms use diffusion to transport molecules into the body from the air as they have a large surface area : volume ratio
Diffusion + concentration gradient: the greater the difference in concentration, the quicker the rate of diffusion (more particles are moving down the gradient)
Diffusion + temperature: the greater the temperature, the quicker the rate of diffusion (particles have more kinetic energy so move faster and collide frequently)
Diffusion + surface area : volume ratio: the greater the surface area, the quicker the rate of diffusion (particles have more space to move through)
Diffusion + distance: the lower the distance particles need to travel, the quicker the rate of diffusion
Active transport: the movement of particles from an area of low concentration to an area of high concentration using energy
Active transport example: root hair cells use diffusion to take mineral ions from the soil using energy as cells have a higher concentration of mineral ions
Diffusion exp: cut out 1cm cubed agar cube, place in HCl, remove + wash the cube, cut cube in half, measure diffusion distance, use different concentrations of HCl

2.18-2.33 Nutrition

Carbohydrates: bread, pasta, potatoes, for energy
Proteins: meat, fish, eggs, for growth and repair
Lipids: butter, oil, cheese, for energy and insulation
Fibre: vegetables, bran, for pushing food down the gut + preventing constipation
Vitamin A: carrots, for vision
Vitamin C: citrus fruits, for absorbing iron + preventing scurvy
Vitamin D: margarine, for absorbing calcium
Calcium: milk, for bone and teeth strength + preventing rickets
Iron: red meat, for haemoglobin + preventing anaemia
Water: water, juice, milk, for cell reactions to take place
Age: energy requirements increase when approaching adulthood then decreases as adults age
Activity levels: energy requirements increase because you need energy for movement
Pregnancy: energy requirements increase to support growth of the foetus
Mouth: mechanical when teeth break down food pieces, chemical when amylase breaks down starch, salivary glands produce saliva to lubricate the food bolus
Oesophagus: tube from mouth to stomach, bolus moves down due to wave-like contractions (peristalsis) which squeeze the food down
Pancreas: produces carbohydrates, protease and lipase and secretes these enzymes into the stomach and small intestine
Stomach: pepsin breaks down proteins, hydrochloric acid kills bacteria, muscular walls churn food
Liver: produces bile (alkaline) to neutralize HCl and to emulsify fat which increases surface area and it is broken down quicker, stored in gall bladder
Small intestine: enzymes break down food, molecules diffuse into the bloodstream via the villi, bile enters via the bile duct
Villi: thin walls decreases diffusion distance, rich capillary network to carry digested food, microvilli to increase surface area, small intestine is long
Large intestine: absorbs water, produces faeces, faeces are stored in the rectum, faeces are removed through the anus
Carbohydrates: starch to maltose by amylase, maltose to glucose by maltase, broken down in mouth + small intestine
Proteins: proteins to amino acids by proteases, broken down in stomach + small intestine
Lipids: lipids to glycerol + 3 fatty acids by lipases, broken down in small intestine
Energy exp: measure temperature of cold water, measure mass of food sample, heat food till it catches fire, put sample under water, measure temperature of water
Energy exp: independent - food sample, dependent - temperature rise, control - volume of water, distance from food to calorimeter
Energy equation: Energy transferred (J) = Temperature increase (degrees C) x Mass (g) x 4.2 (J/g/degrees C)

2.34-2.39 Respiration

Respiration: release of energy within the cell either aerobically or anaerobically
Aerobic: Glucose + Oxygen > Water + Carbon Dioxide (C6 H12 O6 + O2 > CO2 + H2O), uses oxygen, full breakdown of glucose, releases energy used to make ATP
Anaerobic (animals): Glucose > Lactic acid, very low energy release, partial breakdown of glucose, very less ATP produced
Anaerobic (plants + fungi): Glucose > Ethanol + Carbon Dioxide (C6 H12 O6 > C2 H5 OH + CO2), low energy release, partial breakdown of glucose, less ATP produced
Energy: ATP is used for other processes in the cell (e.g. active transport, protein synthesis, muscle contraction)
Evolution of CO2 exp: flask 1 limewater, flask 2 germinating seeds, flask 3 limewater, connect flasks with capillary tubes
Evolution of CO2 exp: flask 1 remains clear - air doesn't have enough CO2 to affect limewater, flask 3 goes cloudy - organisms are respiring + producing CO2
Evolution of CO2 exp: independent - living or not living, dependent - colour of limewater in flask 3, control - temperature, volume and concentration of limewater
Evolution of heat exp: flask 1 germinating seeds, flask 2 dead seeds, measure initial temperature, leave for few days, measure end temperature
Evolution of heat exp: temperature will increase for live germinating seeds as they release heat from aerobic temperature
Evolution of heat exp: independent - living or not living, dependent - temperature, control - number of seeds

2.40-2.50 Gas Exchange

Ribs: bone cage surrounding the lungs to provide protection of internal organs
Intercostal muscles: muscles found between the ribs that control inhalation and exhalation
Diaphragm: dome at the bottom of the thorax that changes the pressure to control inhalation and exhalation
Trachea: the windpipe where air enters the thorax and flows to the lungs
Bronchi: two tubes to each lung
Bronchioles: smaller tubes that connect to the alveoli
Alveoli: tiny air sacs that are the place of gas exchange
Pleural membranes: found on the outside of the lungs to lubricate them (reduce friction while breathing)
Inhalation: intercostal muscles contract, the ribcage moves up and out, the diaphragm contracts downwards, pressure decreases, air moves in
Exhalation: intercostal muscles relax, the ribcage moves down and in, the diaphragm relaxes upwards, pressure increases, air moves out
Alveoli: thin walls so short diffusion distance, large surface area so more space for molecules to travel through, good blood supply so maintains the concentration
Exercise exp: We'll change whether the student has exercised or not. The students will be of the same age, gender, size and general fitness. We'll repeat the investigation several times to ensure our results are reliable. We'll measure the change in breathing rate. Immediately after exercise and each minute for the subsequent 5 minutes. We'll control the type of exercise carried out, the temperature of the environment, the food intake of the students prior to the investigation.

2.51-2.69 Transport

Unicellular organisms: diffusion to transport molecules, high surface area to volume ratio, more space for molecules to travel at once + short diffusion distance
Multicellular organisms: transport system to transport molecules, low surface area to volume ratio, less space for molecules to travel at once + larger distance

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