16353382
Description
Flashcards by Ellie Potellie, updated more than 1 year ago
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Created by Ellie Potellie
almost 6 years ago
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| Question | Answer |
| What Enzyme Is Responsible For DNA Replication? | DNA Polymerase |
| What Is Meant By Continuous Synthesis? | Whole strand is made in one piece |
| What Is Meant By Dis-Continuous Synthesis? | Strand is replicated in fragments |
| What Is The Function Of PCR? | To amplify a sequence of DNA |
| Why Is DNA Heat Back Up For The Third Stage Of PCR? | To allow taq polymerase to synthesise the new strands of DNA |
| Why Is DNA Cooled During The Second Stage Of PCR? | To allow primers to anneal to the template strands |
| Why Is Taq Polymerase Used In PCR? | It doesn't denature during heating cycles |
| Which Rule Allows The Molecules of DNA To Be Copied? | Complementary base pair rule |
| What Temperature Does The Second Stage Of PCR Occur At? | 55^* and 65^* (Any Within Range) |
| What Temperature Does The First Stage Of PCR Occur At? | 94^* & 96^* (Any Within Range) |
| Why Is DNA Heated In The First Stage Of PCR? | To break the hydrogen bonds between the bases and separate the two strands |
| Which Bonds Are Broken To Separate Two Strands Of DNA? | Hydrogen Bonding |
| Which Enzyme Joins The Fragments Of DNA On The Logging Strand? | Ligase |
| What Is The Difference Between DNA Polymerase and Taq Polymerase? | DNA Polymerase denatures at lower temperatures than taq polymerase |
| When Is PCR Used? | Crime scene analysis to identify suspects, paternity testing, identifying evolutionary relationships |
| What Are The Requirements Of PCR? | •Template DNA •Taq Polymerase •Primers •Free DNA nucleotides •Buffer |
| Why Is DNA Replication Required? | Before mitosis to ensure that both daughter cells get an identical copy of the genetic information from the parent cell |
| Why Is There a Leading and Lagging Strand In DNA Replication? | Parent strands are anti-parallel but DNA can only by synthesised in 5 - 9 direction |
| Which Strand In DNA Replication Requires The Use Of Many Primers? | Lagging Strand |
| Why Are Two Different Primers Needed During PCR? | One which is complementary to each strand being copied |
| What Is The Function Of A Primer? | To Provide a start point for DNA polymerase |
| Why Can DNA Only Be Replicated In One Direction? | Because DNA Polymerase can only add nucleotides to the 3" end |
| What Temperature Does The Third Stage Of PCR Occur At? | 72^* |
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