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| Question | Answer |
| What are the conditions needed for cell fractionation, and why? | Ice cold to reduce enzyme activity Buffered to keep a constant pH In isotonic solution to prevent damage to the cell through osmosis |
| What is the first step of cell fractionation? | Homogenisation- break up the cells |
| What is the second step of cell fractionation? | Filtration- Filter the homogenised cell solution to remove large cell debris or tissue debris |
| What is the third step in cell fractionation? | Ultracentrifugation- Put the solution into a centrifuge. Once done, drain off the supernatant into another tube and spin it again. You can discard the pellet |
| In what order do organelles separate at? | Nuclei, chloroplasts, mitochondria, lysosomes, endoplasmic reticulum, ribosomes |
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