812078
Description
Flashcards by Jumai Abioye, updated more than 1 year ago
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Created by Jumai Abioye
over 10 years ago
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| Question | Answer |
| Amtmann and Leigh, 2009 | all |
| James et al., 2006 | Exploiting natural variation; Na Extrusion/Exclusion :Durum wheat; identified two HKT type transporters, Nax1 and Nax2 using genetic crossing, qtl mapping genetic analysis of of segregating populaiton; restrict Na+ transport from roots to shoots and result in enhanced K1-Na1 discrimination in the leaf blade through Na+ withdrawal from xylem; Nax1 also led to accumulation of Na+ in leaf sheath |
| Munns et al., 2000 | Refer to James et al., 2006: they identified the candidate genes Nax1 and Nax2: compared with rice loci: Durum wheat |
| Apse et a., 1999 | Na Compartmentalization: Arabidopsis thaliana NHX1 (vacuolar Na/H antiporter): Used ELISA and pH dependent fluorescence quenching of acridine orange: electroneutral exchange// overexpression in A.thaliana= increased tolerance |
| Zhang and Blumwald, 2001 | Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit/ overexpressing NHX1 vacuolar Na/H antiporter/ western blot and pH-dependent fluorescence quenching of acridine orange (transport assay) |
| Gaxiola et al., 2001 | COMPARTMENTALIZATION: Transgenic plants overexpressing the vacuolar H1-pyrophosphatase AVP1 (H+ppase) more salt tolerant: it pumps protons into vacuole: Increased proton gradient prob drives Na+ uptake through NHX Na/H antiporter into vacuole |
| Na EXCLUSION | No transgenic plant yet. Several pathways involved? Several candidates: HKT: Na/K(Na) co-transporters. 1 gene in A. thaliana but many more in rice & wheat. CNGC: putative cyclic-nucleotide gated channels. 20 genes in A. thaliana. GLR: putative glutamate receptors. 20 genes in A. thaliana. |
| Moller et al., 2009 | EXCLUSIONCell type specific overexpression of HKT1;1 in stelar root cells led to shoot Na exclusion and accumulation of Na in root, also in better K uptake in shoot. Used enhanced trap system to drive cell specific expression. Constitutively expressed HKT1;1 using CaMV promoter resulted in Na accumulation in shoot and stunted growth. |
| Volkov V & Amtmann A (2006) | EXCLUSION?? T. halophila more tolerant than A. thalianaIon // channels in root cells of Thellungiella are more selective for K over Na than in Arabidopsis. (NSC) |
| Liu and Zhu, 1998 | NA EXTRUSION: Root bending Assay: SOS1: Plasma membrane Na/H antiporter /SOS2: Ca-dependent protein kinase/ SOS3: Ca binding protein |
| Shi et al., 2002 | EXTRUSION: SOS1/SOS2/SOS3: Heterologous expression in yeast mutants/ Confocal imaging/Gus analysis: Dual function of SOS1- loading xylem under mild stress and unloading under severe salt stress- Na |
| Quintero et al., 2002 | Functional reconstitution of the SOS pathway in yeast demonstrates that the genes act on the same pathway. Also constitutively active SOS2 complex (thr to asp) was found to substitue for SOS2/SOS3 complex but not completely in the activation of SOS1 |
| Waditee et al., 2005 | COMPATIBLE SOLUTE: GLYCINE BETAINE Coexpression of two N-methyltransferases from salt water cyanobacteria in fresh water cyanobacteria and Arabidopsis conferred increased salt tolerance by accumulation of GB. more GB was accumulated than when produced by two step oxidation of choline. |
| Waditee et al., 2007 | an exogenous supply of serine or glycine to a halotolerant cyanobacterium Aphanothece halophytica, which synthesizes betaine from glycine by a three-step methylation, elevated intracellular accumulation of betaine under salt stress. |
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