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11830975
DISAGGREGATION OF EXPLANT
Description
Flowchart on DISAGGREGATION OF EXPLANT, created by Aaff rin on 03/01/2018.
Flowchart by
Aaff rin
, updated more than 1 year ago
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Created by
Aaff rin
almost 7 years ago
12
1
0
Resource summary
Flowchart nodes
DISAGGREGATION OF EXPLANTS
FINE DISSECTION
MECHANICAL DISAGGREGATION
COLD TRYPSIN
WARM TRYPSIN
COLLAGENASE
DISPERSED PRIMARY CULTUTURE
SUBCULTURE
PRIMARY EXPLANT
TRANSFER
2ND EXPLANT
OVER NIGHT INCUBATION
LONG INCUBATION
CENTRIFUGE
CELL LINE
OUTGROWTH
RESUSPEND AND SEED
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