null
US
Sign In
Sign Up for Free
Sign Up
We have detected that Javascript is not enabled in your browser. The dynamic nature of our site means that Javascript must be enabled to function properly. Please read our
terms and conditions
for more information.
Next up
Copy and Edit
You need to log in to complete this action!
Register for Free
25053641
RT PCR METHOD
Description
biologia molecular
No tags specified
biologia
Flowchart by
Manuel Enrique Alavrez
, updated more than 1 year ago
More
Less
Created by
Manuel Enrique Alavrez
almost 4 years ago
2
0
0
Resource summary
Flowchart nodes
Metodología a seguir para detección de ARNm de VPH
1.-Se toman muestras del cervix y se almacenaron a (-80ºC)
2.- El ARN de todas las células se preparó usando Trizol (Life Technologies, Carlsbad, CA) d
3.- Se preparan ADNc (complementario) en sistema de síntesis de primera cadena SuperScript III para RT-PCR
4.- Uso de PCR semicuantitativa para amplificar simultáneamente el ADNc celular de GAPDH y HPV L1.
5.-Analisis de muestras
Uso de centrifugación para sedimentar ARN
Analisis de pureza por AGILENT 2100
Rtpcr Cebadores (binary/octet-stream)
síntesis de ADNc se realizó en ácido nucleico extraído tratado con DNase-treated
TRANSCRIPCIÓN INVERSA
Se multiplicaron las cadenas de ADNc
REPLICACIÓN DE ADNc
Se usaron cebadores y primers para determinar la cantidad en tiempo real de carga viral (ARNm)
Rtpcr Dogma (binary/octet-stream)
Captura1 (binary/octet-stream)
Captura2 (binary/octet-stream)
Captura4 (binary/octet-stream)
Show full summary
Hide full summary
Want to create your own
Flowcharts
for
free
with GoConqr?
Learn more
.
Similar
Spanish Vocabulary- Intermediate
PatrickNoonan
Criminal Law
jesusreyes88
Periodicity
hanalou
AQA GCSE Biology Unit 2.3
Matthew T
English Language Terms
ekimlauretta
AQA Biology B1 Questions
Bella Statham
Structure of the League of Nations
saskiamitchell.19
Musical Terms
Abby B
Biology - the digestive system
Oliviax
AQA GCSE Chemistry Unit 2
Gabi Germain
Groups Starter Pack
Micheal Heffernan
Browse Library