null
US
Sign In
Sign Up for Free
Sign Up
We have detected that Javascript is not enabled in your browser. The dynamic nature of our site means that Javascript must be enabled to function properly. Please read our
terms and conditions
for more information.
Next up
Copy and Edit
You need to log in to complete this action!
Register for Free
8644439
Attachment of Ub to Target Protein
Description
Ubiquitin mechanism for protein targeting
No tags specified
ubiquitin
amp
atp hydrolysis
e1
thioester
transthiolation
e2
e3
cys
lys-48
biology
biochemistry
Flowchart by
Chinazor Okeke
, updated more than 1 year ago
More
Less
Created by
Chinazor Okeke
over 7 years ago
10
0
0
Resource summary
Flowchart nodes
1. -NH of Lys residue on protein is covalently attached to the COO- terminus of Ub
2. Another Ub attaches to the Lys 48 -NH of the first Ub
3. Repeat Steps 2 twice until you get four covalently attached Ubs
4 Ub's = the DEATH MARK
1. E1 activates the Ub by adenylating -COO with AMP (hydrolysis of ATP into AMP and PPi)
2. E1 transfers Ub onto its Cys residue to form a high E thioester bond, thereby removing AMP
3. A transthiolation reaction transfers an activated Ub from E1 to E2
E2 is now bonded to Ub via a thioester bond
4. E3 recognizes and associates with both the E2-Ub complex and the target protein
E1 = Ub activating enzyme
E2 = Ub conjugating enzyme
5. E3 catalyzes the transfer of Ub onto amino groups on specific Lys on the target protein
6. W/ E3 still bound to the target protein, repeat steps 1-5 until 3 more Ubs are attached
E3 = scaffold b/c it recognizes both target protein and E2-Ub complex
E3 = Ub ligating enzyme
Ubiquitin Targeting and Mechanism
Show full summary
Hide full summary
Want to create your own
Flowcharts
for
free
with GoConqr?
Learn more
.
Similar
DNA (labeling) for biochem and cell biology (lecture 2)
MrSujg
Сells and development lecture 1 +organelles
MrSujg
Cell Lecture 3
MrSujg
Biology AQA 3.1.3 Cells
evie.daines
Biology AQA 3.2.5 Mitosis
evie.daines
Biology AQA 3.1.3 Osmosis and Diffusion
evie.daines
Biology- Genes, Chromosomes and DNA
Laura Perry
Biology- Genes and Variation
Laura Perry
Enzymes and Respiration
I Turner
GCSE AQA Biology - Unit 2
James Jolliffe
GCSE AQA Biology 1 Quiz
Lilac Potato
Browse Library