Th1L07 Clinical use of enzyme measurements

Appearance of creatine kinase and lactate dehydrogenase in plasma after myocardial infarction
1. Increase in CK2 activity 24 hours after
2. Increase in LDH activity 48 hours after
  1. Isozymes of lactate dehydrogenase can be separated by chromatography
    1. five different isozymes of lactate hydrogenase exist
      1. H4, H3M, H2M2, HM3 and M4
    2. During a myocardial infarction
      1. Endothelial cells rupture, releasing their contents into the blood stream
        This increases the serum levels for lactate hydrogenase isozyme 1 and 2 and cane be used as a diagnostic tool
  2. composed of four monomers
    1. heart or muscle type
Clinical uses of enzyme inhibitors
1. Control of angiotensin production
  1. cleavage of Angiotensin I to Angiotensin II results in peripheral vasoconstriction and aldosterone secretion
    1. Treatment of heart failure with ACE inhibitors prevents sodium/water retention > oedema
Enzyme inhibitors as toxins
1. Mustard gas, sarin (nerve gases)
  1. Organophosphorus
    1. Inhibits cholinesterase irreversibly; poisons the enzyme

Resource summary

	Created by Emma Allde about 8 years ago