Totipotency & Tissue Culture

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AS Level AS Biology (IAL AS Biology Unit 3 ) Note on Totipotency & Tissue Culture, created by AD_ on 23/04/2014.
AD_
Note by AD_, updated more than 1 year ago
AD_
Created by AD_ over 10 years ago
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Page 1

Method: Sprinkle seeds on damp sponge & allow germinate. Use when seeds become sapling. Make up agar gel into test tube. With sharp scissors cut tops off below the shoot apex. Push stem of explants into the gel, cover with cling film & place on sunny windowsill. Observe over 10 days.

Outcome:Explants grow roots & leaves continue grow. 

Evaluation Issues: Wrong part plant cut & inserted into gel. Unwanted pathogens grow in  gel as it is good source of water & nutrients.

Method

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