Pigments in histopathology

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What pigment is deposited in this picture?

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Von Kossa's method is for calcium

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Hemosiderin is detected using ❌ Reaction. Ferric iron deposits in tissue react with ❌ ferrocyanide to form ❌ prussian blue dye

Perls

alizarin red

soluble

insoluble

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Copper; (choose all correct options)

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Anthracosis is also known as carbon lungs

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Asbestosis is covered in iron containing protein coat; use Perls stain

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Talc is biofringent

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What colour is embolisation pigment?

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Tattoo pigments in skin tissue microscopy can be problematic with the diagnosis of

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Cholesterol ❌ are due to ❌ and alcohol dissolving cholesterol crystals.

clefts

domes

xylene

acid

Eosin

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Melanin is an endogenous pigment

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pigments can be detected with Fouchet's bile stain.

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What is Perls reaction positive for?

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What is Autofluorescence positive for?

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What is Schmorl's positive for?

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melanin is a tyrosine based pigment

1

The two types of melanin are and

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( Argentaffilic, Argyrophilic ) reaction binds silver and reduces it to black, whilst ( Argyrophilic, Argentaffilic ) binds silver but needs an external reducer such as formalin. (e.g. reticulin stain)

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Melanin is Argentaffilic

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Hydrogen peroxide bleach can be done to remove pigment

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Choose correct information in regards to Dermal melanin.

1

Tick boxes below that are classified as aging pigments

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Corpora Amylacea is PAS negative

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Amyloid pigment is ❌ in polarised light after Congo red staining. It is an ❌ and proteinaceous. Several techniques should be used such as IHC and Crystal Violet for ❌.

birefringent

invisible

metachromasia

polychromasia

extracellular

intracellular

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What pigment is usually found in "Brown atrophy" of heart, adrenal glands and hepatic parenchymal cells? (They are gold/brown pigments)

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What are lipofuscin positive for?

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Schmorle's reaction demonstrates oxidising substances

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( Melanin, Lipofuscin, Hemosiderin, Bile ) is Argentaffin and Schmorles positive, DiPAS negative and removed by H2O2 bleach. It is Long ZN negative, fat stain and Luxol Fast Blue negative. ( Lipofuscin, melanin, hemosiderin, bile ) on the other hand can be positive or negative Argentaffin and Schmorle's depending on timing, DiPAS positive, H2O2 bleach does not remove it, and positive for Long ZN, fat stains and Luxol fast blue.

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Quiz on Pigments in histopathology, created by Laura Currie on 28/10/2018.

Pigments in histopathology

What pigment is deposited in this picture?

Von Kossa's method is for calcium

Hemosiderin is detected using ❌ Reaction. Ferric iron deposits in tissue react with ❌ ferrocyanide to form ❌ prussian blue dye

Copper; (choose all correct options)

Anthracosis is also known as carbon lungs

Asbestosis is covered in iron containing protein coat; use Perls stain

Talc is biofringent

What colour is embolisation pigment?

Tattoo pigments in skin tissue microscopy can be problematic with the diagnosis of

Cholesterol ❌ are due to ❌ and alcohol dissolving cholesterol crystals.

Melanin is an endogenous pigment

pigments can be detected with Fouchet's bile stain.

What is Perls reaction positive for?

What is Autofluorescence positive for?

What is Schmorl's positive for?

melanin is a tyrosine based pigment

The two types of melanin are and

Argentaffilic Argyrophilic( Argentaffilic, Argyrophilic ) reaction binds silver and reduces it to black, whilst Argyrophilic Argentaffilic( Argyrophilic, Argentaffilic ) binds silver but needs an external reducer such as formalin. (e.g. reticulin stain)

Melanin is Argentaffilic

( Argentaffilic, Argyrophilic ) reaction binds silver and reduces it to black, whilst ( Argyrophilic, Argentaffilic ) binds silver but needs an external reducer such as formalin. (e.g. reticulin stain)