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Histotechnology Quiz on Histotechnology, created by Victoria Station on 04/12/2018.

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Histotechnology

Question 1 of 90

1

What would not be seen in autolytic tissue?

Select one of the following:

  • Nuclear detail lost

  • Stainability lost

  • Stain precipitation

  • Cells coming away from basement membrane

Explanation

Question 2 of 90

1

What would be a "normal" specimen received in the grossing room?

Select one of the following:

  • Tumor

  • Placenta

  • Surgical

  • Biopsy

Explanation

Question 3 of 90

1

Cassettes:

Select one of the following:

  • Are 3 cm deep

  • Must be labeled

  • Colours indicate day of week the tissue was processed

  • Has holes to allow the tissue to flow in an out

Explanation

Question 4 of 90

1

Fixation:

Select one of the following:

  • Functions by creating hydrogen bridges to prevent degredation

  • Typically shrinks, swells, and distorts tissue

  • Keeps bacteria and fungi viable

  • Allows the tissue to be stainable

Explanation

Question 5 of 90

1

Which is not a factor affecting fixation?

Select one of the following:

  • Time

  • Density of tissue

  • Temperature

  • Type of container

Explanation

Question 6 of 90

1

Which is not a coagulant fixative?

Select one of the following:

  • Zinc

  • Picric Acid

  • Acetic Acid

  • Alcohol

Explanation

Question 7 of 90

1

Which non-coagulant fixative is also non-additive

Select one of the following:

  • Osmium Tetroxide

  • Acetic Acid

  • Formalin

  • Gluteraldehyde

Explanation

Question 8 of 90

1

How much concentrated formalin is needed to make 5.5L of 10% formalin?

Select one of the following:

  • 55 ml

  • 55 L

  • 5500 ml

  • 550 ml

Explanation

Question 9 of 90

1

To make 10% formalin, how many ml should be added to 230 ml of 100% formalin?

Select one of the following:

  • 2300 ml

  • 0.23 L

  • 2070 ml

  • 2600 ml

Explanation

Question 10 of 90

1

Which is true about formaldehyde?

Select one of the following:

  • It is a coagulant fixative

  • It is composed of liquid formaldehyde in water

  • Add methanol to stop the formation of paraformaldehyde

  • Penetrates tissue slowly but fixes quickly

Explanation

Question 11 of 90

1

The best fixative for DNA/RNA is?

Select one of the following:

  • Carnoy

  • Picric Acid

  • Formalin

  • Gluteraldehyde

Explanation

Question 12 of 90

1

Which is false about gluteraldehyde?

Select one of the following:

  • Is a non-coagulant, additive fixative

  • Can be used to disinfect the cryostat

  • Can cause false positive reactions in period acid schiff

  • Creates methylene bridges in tissue to preserve it

Explanation

Question 13 of 90

1

Mercuric Chloride

Select one of the following:

  • Is a component in B5 fixative

  • Is a common fixative used in the histology lab

  • Mercuric pigment can be removed using alcoholic picric acid

  • Is a great fixative when tissue needs to be X-rayed

Explanation

Question 14 of 90

1

What is false about osmium tetroxide?

Select one of the following:

  • Fixes lipids

  • Used mostly for electron microscopy

  • Commonly a secondary fixative

  • Is a non-additive fixative

Explanation

Question 15 of 90

1

Bouin's fixative:

Select one of the following:

  • Is composed of picric acid, formaldehyde and mercuric chloride

  • Is the best preservative for glycogen

  • Does not stain tissue

  • Is commonly used as a secondary fixative

Explanation

Question 16 of 90

1

Which is known to be a good general fixative?

Select one of the following:

  • Gluteraldehyde

  • PAF / Zamboni

  • Picric Acid

  • Mercuric Chloride

Explanation

Question 17 of 90

1

Select the correct statement regarding non-aqueous fixatives?

Select one of the following:

  • Acetone should not be used for IHC since it destroys enzymes

  • Acetone is a good fixative since it does not shrink or harden tissue

  • Ethanol should not be used to fix frozen sections since it inhibits freezing

  • Ethanol should not be used to preserve glycogen as it is known to dissolve it

Explanation

Question 18 of 90

1

Which fixative would be a good choice when RBCs need to be preseved

Select one of the following:

  • Carnoy's

  • Picric Acid

  • Bouin's

  • Acetic Acid

Explanation

Question 19 of 90

1

Oh no! Your tissue is autolytic! What should be done to prevent this?

Select one of the following:

  • Tissue should be placed in fixative 10X greater than the tissue itself

  • Tissue should have been placed in fixative right after removal from the body

  • Tissue in fixative should be placed at 4 degrees Celcius

  • Tissue should have been frozen before fixation

Explanation

Question 20 of 90

1

What would be proper fixation procedure for a large tissue?

Select one of the following:

  • Large tissue should not be fixed

  • Large tissue must be cut into smaller pieces

  • Holes must be poked in the specimen to allow fixative to penetrate

  • Fixative must be injected into the specimen

Explanation

Question 21 of 90

1

Which is true regarding the VIP Tissue Processor?

Select one of the following:

  • Uses vacuum technology to infiltrate tissues

  • Hematoxylin is added to tint the tissue pink for easier embedding orientation

  • This processor is safer since it does not use heat

  • This is an open system

Explanation

Question 22 of 90

1

Which is TRUE about dehydration?

Select one of the following:

  • The dehydration process begins with the highest alcohol concentration then descends

  • Ethyl alcohol is the best for dehydration

  • Butanol is a good substitution because it is quick

  • Alcohol dehydrates tissue by removing xylene

Explanation

Question 23 of 90

1

How would you be able to tell if a tissue was improperly dehydrated?

Select one of the following:

  • Tissue would smell like xylene

  • Tissue would be soft

  • Impossible to tell until staining

  • Tissue would be dessicated

Explanation

Question 24 of 90

1

What is the proper way to fix improper dehydration?

Select one of the following:

  • First xylene, then several changes of alcohol, then xylene, then wax

  • Impossible to fix, request new specimen

  • First several changes of alcohol, then xylene, then wax

  • Several changes of wax

Explanation

Question 25 of 90

1

After processing; tissue is soft, cloudy, and smells like alcohol. Why?

Select one of the following:

  • Improper fixation

  • Improper dehydration

  • Improper clearing

  • Improper infiltration

Explanation

Question 26 of 90

1

Which is not a clearing agent?

Select one of the following:

  • Xylene

  • Toluene

  • Benzene

  • Chloridine

Explanation

Question 27 of 90

1

Which is true considered a universal solvent?

Select one of the following:

  • An example is Limonene

  • A solution that dehydrates and clears

  • An example is Xylene

  • A solution that dehydrates and infiltrates

Explanation

Question 28 of 90

1

How would you fix improper infiltration?

Select one of the following:

  • Increase the temperature of the wax

  • Remove wax with xylene, dehydrate with alcohol, then clear with xylene, then several changes of wax

  • Re-fix the tissue

  • Several changes of wax

Explanation

Question 29 of 90

1

Which statement is TRUE?

Select one of the following:

  • Soft wax allows for easy ribboning

  • Soft wax provides the most support

  • Hard wax melts at 45 degrees Celsius

  • Hard wax should not be used if thinner sections are required

Explanation

Question 30 of 90

1

What is true about paraffin wax?

Select one of the following:

  • Wax should be kept at least 10 degrees over the melting point

  • Typical melting point is 35-48 degrees

  • Overexposure to wax will cause tissue hardening and shrinking

  • Water-bath should be kept 2 degrees above the melting point

Explanation

Question 31 of 90

1

What is an advantage to using a microwave for processing?

Select one of the following:

  • Clearing step can be skipped

  • Infiltration step can be skipped

  • Dehydration step can be skipped

  • Microwaves would never be used for processing

Explanation

Question 32 of 90

1

A technologist is viewing several embedded tissues. Which one would need to be re-embedded?

Select one of the following:

  • Several small biopsies are placed together

  • Red dot is facing towards you when cutting

  • Two pieces of small bowel are orientated so that the lumens are facing eachother

  • Transverse cut of tubular tissue

Explanation

Question 33 of 90

1

Which of these is proper troubleshooting?

Select one of the following:

  • Incorrect orientation of tissue when embedding - discard the tissue and request new specimen

  • Poor processing of tissues - check solutions and machine

  • Tissue is over-dehydrated - increase time in alcohol

  • NBF precipitate is found in the tubing of the processor - perform a picric acid flush

Explanation

Question 34 of 90

1

Troubleshoot this cutting situation: what is the most likely cause of crooked ribbons?

Select one of the following:

  • Block not chilled enough

  • Cutting too slowly

  • Dull blade

  • Block and knife not parallel to eachother

Explanation

Question 35 of 90

1

Select the correct statement about decalcification

Select one of the following:

  • Tissue must be > 3 mm in order to be decalcified properly

  • Calcium is soluble at pH 4.5

  • Acid methods are usually slow

  • Decalcification increases stainability of bone tissue

Explanation

Question 36 of 90

1

What is the most accurate way to determine the end point of decalcification?

Select one of the following:

  • Mechanical methods such as poking

  • Chemical methods such as ammonium hydroxide and ammonium oxalate which check for the precipitation of calcium oxalate

  • Cutting the bone tissue

  • Radiography

Explanation

Question 37 of 90

1

When looking at a H&E stained bone under the microscope, the technologist notices that there is many dark blue/purple staining areas. What could this mean?

Select one of the following:

  • Bone was stained too long in hematoxylin

  • Bone was underdecalcified

  • Bone was improperly fixed

  • Bone was not stained with the correct stain

Explanation

Question 38 of 90

1

What is the incorrect procedure when using the cryostat?

Select one of the following:

  • Disinfect daily with sodium hypochlorite

  • Use cryostat when rapid diagnosis is required or when fat or enzymes need to be demonstrated.

  • If a tissue with known TB requires cutting, disinfect afterwards with formaldehyde vapor

  • If tissue is sticking the antiroll plate may be too warm

Explanation

Question 39 of 90

1

Which is the correct use for each microscope?

Select one of the following:

  • Fluorescent microscope for Acid Fast Bacilli

  • Polarizing microscope for Oil Red O

  • Compound light microscope for Acridine Orange

  • Electron microscope for H&E

Explanation

Question 40 of 90

1

Select the correct statement about microtomes:

Select one of the following:

  • "Roughing in" is usually done at 20 micrometers

  • The clearance angle is usually set at 10 degrees

  • Speed of cutting has no effect on ribbon quality

  • In a rotary microtome, the knife moves side to side

Explanation

Question 41 of 90

1

Select the correct statement about automatic stainers:

Select one of the following:

  • With linear stainers, time in solution can be varied by adding more containers

  • With robotic stainers, the programs cannot be changed

  • Robotic stainers are good for progressive staining

  • Linear stainers are good for regressing staining

Explanation

Question 42 of 90

1

What is proper water bath procedure?

Select one of the following:

  • Water bath should be kept at 5 - 10 degrees above the melting point of wax

  • Tap water should always be used to fill water baths

  • Parched earth artifact can result if the water bath is too cold

  • Water bath should be cleaned after each ribbon

Explanation

Question 43 of 90

1

A technologist is cutting many tissues for different areas of the lab. Which of the following is an incorrect procedure?

Select one of the following:

  • Slides for IHC were placed on a hotplate for drying.

  • Slides with biospy were cut using special techniques with 6 tissues per slide

  • Slides for neurology were placed in a 37 degree oven for drying

  • Slides for routine H&E were placed in a 60 degree oven for drying

Explanation

Question 44 of 90

1

A student is concerned about contracting TB from patient samples. What process would be of the least concern to the student?

Select one of the following:

  • Embedding

  • Grossing

  • Autopsy

  • Frozen sectioning

Explanation

Question 45 of 90

1

Which is considered to be a correct safe procedure?

Select one of the following:

  • Placing used blades in the garbage

  • Fixing tissue that is known to be TB positive with formalin

  • Storing glacial acetic acid on the counter top

  • Fixing CJD positive tissue with formalin

Explanation

Question 46 of 90

1

Which is not an element of a dye molecule?

Select one of the following:

  • metachrome

  • benzene ring

  • auxochrome

  • chromaphore

Explanation

Question 47 of 90

1

What is a correct statement about staining:

Select one of the following:

  • A method of nuclear staining is acidic dye

  • A method of nuclear staining is dye + metal mordant

  • Cytoplasmic staining occurs from the interaction of dye with neutral molecules

  • Lipids are stained because the dye has a greater affinity to the solution than the fat

Explanation

Question 48 of 90

1

Which is considered a basic auxochrome?

Select one of the following:

  • -NH2

  • -COOH

  • -OH

  • -SO4

Explanation

Question 49 of 90

1

Which statement is correct?

Select one of the following:

  • Basic dyes will have a negative auxochrome

  • Basic dyes will produce chloride salt

  • Acid dyes are cationic

  • Acid dyes produce magnesium salt

Explanation

Question 50 of 90

1

Which is a true statement about dyes?

Select one of the following:

  • Modifiers will affect staining time

  • Sulphonics makes acidic dyes basic

  • Dye binding can be affected by pH

  • Orthochromic dye will stain a different colour than the dye colour

Explanation

Question 51 of 90

1

Which is not a metachromatic component?

Select one of the following:

  • AFB

  • Mucin

  • Mast Cell

  • Amyloid

Explanation

Question 52 of 90

1

Why are thymol crystals added to dye? ;)

Select one of the following:

  • Prevent mold growth

  • Enhance staining

  • Makes the dye more acidic

  • Makes the dye fluorescent

Explanation

Question 53 of 90

1

Which is not a natural dye?

Select one of the following:

  • Hematoxylin

  • Orcein

  • Acridine Orange

  • Saffron

Explanation

Question 54 of 90

1

Which pairing for decolorization is incorrect?

Select one of the following:

  • Weak acid differentiating basic dyes ex. PTA/PMA for Biebrich Scarlet

  • Weak base differentiating acid dyes ex. PTA/PMA for Biebrich Scarlet

  • Excess mordant differentiating ex. iron alum for Verhoeff

  • Alcoholic differentiation ex. alcohol for eosin

Explanation

Question 55 of 90

1

A technologist needs to prepare a hematoxylin for use in Masson's Trichrome. Which would be the correct procedure?

Select one of the following:

  • Oxidize the hematoxylin with sodium iodate

  • Prepare hematoxylin using aluminum since it can withstand strong acids in the stain

  • After preparing the hematoxylin, pour directly into the coplin jar for staining

  • Gill's hematoxylin should be prepared for this stain

Explanation

Question 56 of 90

1

A technologist has prepared several different hematoxylins for use. Which one of them is paired correctly for use?

Select one of the following:

  • Gill's hematoxylin for use in Alcian Blue

  • Mayer's hematoxylin for Oil Red O

  • Harris hematoxylin for Masson's Trichrome

  • Weigert's hematoxylin for routine H&E

Explanation

Question 57 of 90

1

What is true about Hematoxylin and Eosin?

Select one of the following:

  • Hematoxylin stains indirectly

  • Is never a regressive stain

  • Cannot be used to stain bone tissue

  • The best substitute for eosin would be phenol red

Explanation

Question 58 of 90

1

A technologist is reviewing a manual H&E control slide when they notice that the nuclei have not stained. Which of the following options is likely NOT be a reason for this problem.

Select one of the following:

  • Tissue was left in acid alcohol for 20 minutes during coffee break

  • Aluminum / iron was not present in the hematoxylin

  • The hematoxylin was not filtered properly

  • The hematoxylin was stored in a clear glass jar

Explanation

Question 59 of 90

1

A technologist reviewing an H&E control has noticed that the cytoplasm is staining very dark. Which is not a likely cause?

Select one of the following:

  • Tissue was cut at 3 micrometers

  • Eosin is too concentrated

  • Eosin was not left in alcohol long enough

  • Tissue was left in eosin for too long

Explanation

Question 60 of 90

1

A technologist is preparing to perform Masson's trichrome on a kidney slide. Which of the following procedures is INCORRECT?

Select one of the following:

  • Places slide in Bouin's for 1 hour at 56 degrees.

  • Counterstain in Light Green

  • Places slides in Biebrich Scarlet Acid Fuchsin before PTA/PMA

  • Uses Mayer's Hematoxylin to stain nuclei

Explanation

Question 61 of 90

1

A technologist is reviewing the Masson's Trichrome control. Which of the following stained components is cause for concern?

Select one of the following:

  • Collagen has stained blue

  • Cytoplasm has stained red

  • RBCs have stained red

  • Nucleic acids have stained red

Explanation

Question 62 of 90

1

A technologist is preparing to perform Gordon and Sweets on a liver tissue. Which of the following is the CORRECT procedure?

Select one of the following:

  • Ensure than silver solution is neutralized with HCl before disposal

  • Place the slides in a thin walled coplin jar to ensure good heating in the microwave

  • Bleach tissue in Oxalic Acid after Potassium Permanganate

  • Counterstain in Light Green

Explanation

Question 63 of 90

1

Which staining method is NOT correctly paired with the type of metallic impregnation?

Select one of the following:

  • Gomori Burnter - argentaffin

  • Grocott - argyrophilic

  • von Kossa - substitution

  • Gordon and Sweets - argyrophilic

Explanation

Question 64 of 90

1

A technologist is reviewing the control for Verhoeff's van Gieson. Which of the following errors is paired with the correct solution?

Select one of the following:

  • Elastic fibers cannot easily be seen on the brain control slide so it was placed in Verhoffs stain for a longer period of time

  • Nuclei are not staining so a new solution of Verhoffs made and used

  • The stain looks very muddy so it was placed back into gold chloride for a longer time

  • Elastic fibers are too pale so the slide was placed back into van Gieson stain for longer

Explanation

Question 65 of 90

1

Which is the correct statement about elastic stains?

Select one of the following:

  • Orcein is specific for elastic fibres

  • Orcein will stain elastic fibres brown

  • Aldehyde fuchsin will stain elastic fibres green

  • Aldehyde fuchsin is a regressive method

Explanation

Question 66 of 90

1

A technologist is preparing to perform Toludine Blue. What should the technologist NOT do?

Select one of the following:

  • Take the slides down to water before staining

  • Place the slides in Toludine Blue to stain for mast cells

  • Place the slides in Light Green as a counterstain

  • Differentiate with alcohol then clear and mount

Explanation

Question 67 of 90

1

A technologist is confused why their Alcian Blue control slide did not demonstrate mucins. Which would NOT be a cause for this to happen?

Select one of the following:

  • Stomach tissue was used as the control

  • The tissue was placed in 3% acetic acid before Alcian Blue dye

  • The tissue was placed in Acetic Acid following Alcian Blue for 10 minutes

  • The pH of Alcian Blue was 6.5

Explanation

Question 68 of 90

1

Oh no! Our Periodic Acid Schiff's stain didn't work (again).Which of the following would NOT be a possible cause?

Select one of the following:

  • Schiff's reagent was tested before use using formalin and the reaction became a deep blue colour

  • Grocott's method was performed earlier so the periodic acid solution was saved and used agian

  • Metabisulfite rinse was prepared using 100% HCl straight from the bottle

  • Tissues were washed for 10 minutes in water after Schiff's

Explanation

Question 69 of 90

1

Which statement is true regarding Periodic Acid Schiffs?

Select one of the following:

  • Schiff's reagent is composed of Acid Fuchsin and Sulpheric Acid

  • Periodic acid is the secondary oxidizer

  • Sodium Metabisulfite rise is what brings the pink colour to the tissue

  • Fungi will stain pink

Explanation

Question 70 of 90

1

Which is FALSE about Mayer's Mucicarmine?

Select one of the following:

  • Metanil Yellow is the primary stain

  • Mucicarmine stains acid mucins

  • Gill's hematoxylin should not be used as the nuclear stain

  • Cryptococcus can also be demonstrated with this method

Explanation

Question 71 of 90

1

A technologist is preparing to stain for amyloid. Which procedure is correct?

Select one of the following:

  • The technologist double checked to ensure sections were cut at 4um

  • The doctor would like to differentiate between primary and secondary amyloidosis so the slide was pretreated with potassium permangonate

  • Slides were placed in neutral red to stain for amyloid

  • The technologist did not know how to use the polarizing microscope so birefringence was not checked on the control

Explanation

Question 72 of 90

1

The pathologist has requested an Oil Red O stain for a frozen tissue. Which of the following procedures is INCORRECT?

Select one of the following:

  • The tissue was previously fixed in formalin

  • A frozen section was cut for staining

  • Tissue was taken down to water before staining

  • The tissue was placed in hematoxylin after Oil Red O

Explanation

Question 73 of 90

1

Which is FALSE about Luxol Fast Blue stain?

Select one of the following:

  • Luxol Fast Blue dye is differentiated with an acid

  • The control tissue is cerebellum

  • Eosin is the counterstain

  • Luxol Fast Blue stains myelin

Explanation

Question 74 of 90

1

A technologist notices an endogenous pigment on their slides. What is it?

Select one of the following:

  • mercury

  • tattoo

  • carbon

  • melanin

Explanation

Question 75 of 90

1

A technologist is performing Von Kossa. Which procedure is INCORRECT?

Select one of the following:

  • Tissue is placed in silver nitrate and then microwaved for the appropriate time

  • Placenta is used as the calcium control

  • The tissues are placed in nuclear fast red or neutral red

  • The technologist decides the control passed since the calcium stained brown

Explanation

Question 76 of 90

1

A technologist is performing Perl's Prussian Blue on a control and a patient tissue. The technologist checks the PATIENT'S slide to find that no iron has been stained! What is the most likely cause for this result?

Select one of the following:

  • Slide was placed in eosin

  • Patient tissue does not contain iron

  • Slide was taken down to distilled water

  • Slide was placed in HCl + Potassium Ferrocyanide

Explanation

Question 77 of 90

1

Which is a true statement regarding Gomori Burtner stain?

Select one of the following:

  • This stain is used to demonstrate argentaffin granules

  • Gold chloride is a fixing agent

  • Hematoxylin is the counterstain

  • Melanin cannot oxidize silver therefore a reducer such as Sodium Thiosulfate is required

Explanation

Question 78 of 90

1

A technologist needs to stain for Blastomyces, which stain should they select?

Select one of the following:

  • Ziehl Neelson

  • Gram

  • Geimsa

  • Grocott

Explanation

Question 79 of 90

1

A sample for AFB is arriving in the lab for staining. What should be done?

Select one of the following:

  • After fixation, Gram stain the tissue to detect the bacteria

  • Wear N-95 for frozen section

  • Place the specimen in Carnoy's for fixation then stain with Ziehl Neelson

  • After fixation, take down slides to tap water before staining

Explanation

Question 80 of 90

1

Why do AFB stain red with Ziehl Neelson?

Select one of the following:

  • Not enough Acid Alcohol was used to decolourize the AFB

  • Methylene blue stain was missed

  • Nature of their cell wall

  • Because they are Gram negative

Explanation

Question 81 of 90

1

Which component of Gram stain is correctly matched with its purpose?

Select one of the following:

  • Crystal violet - stains Gram negative bacteria

  • Acetone / Alcohol - decololourizes Gram positive bacteria

  • Basic Fuchsin - stains acid fast bacteria pink

  • Picric Acid - stains bacteria yellow

Explanation

Question 82 of 90

1

A technologist is having trouble with Grocott stain. Which of the following problems is correctly solved?

Select one of the following:

  • Fungi are stained black - the slide is placed back into gold chloride for more toning until fungi are brown

  • Fungi, nuclei, and other tissues are staining black - the slide is good

  • No fungi have been stained - the wrong concentration of periodic acid was used

  • Fungi are brown - the slide is good

Explanation

Question 83 of 90

1

Which of these uncommon stains is correctly paired?

Select one of the following:

  • MSB stain for bile

  • Warthin-Starry for H. pylori

  • Steiner for spirochetes

  • Hall for fibrin

Explanation

Question 84 of 90

1

Which of the following is NOT a correct procedure in IHC?

Select one of the following:

  • Deparaffinize and hydrate to water

  • apply antibody

  • stain nuclei with hematoxylin

  • counterstain with hematoxylin

Explanation

Question 85 of 90

1

Which is true about antigen sites in tissues for IHC?

Select one of the following:

  • Tissue must be washed with water to remove crosslinking from formalin

  • Proteolytic enzymes will destroy the antigen sites

  • Heat methods help cleave the bonds

  • Enzymatic methods will unfold the bonds

Explanation

Question 86 of 90

1

Which of these solutions is NOT properly disposed of?

Select one of the following:

  • Picric acid in a container

  • Alcohol in a container

  • Sodium Thiosulfate down the sink

  • Silver Nitrate down the sink

Explanation

Question 87 of 90

1

Which pairing of control with stain will not work?

Select one of the following:

  • Kidney for Masson's Trichrome

  • Placenta for von Kossa

  • Cerebrum for Luxol Fast Blue

  • Stomach for Alcian Blue

Explanation

Question 88 of 90

1

Why should gloves be worn when cutting tissues on the microtome?

Select one of the following:

  • To prevent the blade from getting scratched

  • To prevent your hands from getting infected

  • To prevent skin cells from getting on the microscope slides

  • To prevent yourself from getting cut

Explanation

Question 89 of 90

1

What would not be a likely reason for seeing pale staining elastic fibres for Verhoeff van Gieson?

Select one of the following:

  • Left in Sodium Thiosulfate for too long

  • Left in Ferric Chloride too long

  • Left in Van Gieson stain too long

  • Left in Verhoeff stain too long

Explanation

Question 90 of 90

1

Which would not be a reason for seeing pale cytoplasmic staining on H&E stained tissue?

Select one of the following:

  • pH of eosin is 8.0

  • Sections were cut at 8 micrometers

  • Tissue left in alcohol for too long

  • Tissues were rinsed in water after blueing

Explanation

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