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Quiz on MCB Midterm Summary, created by Chau Tran on 03/12/2019.

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MCB Midterm Summary

Question 1 of 28

1

A/an _____________ vector quantifies the expression of a gene to measure the activity of regulatory elements.

Select one of the following:

  • Reporter

  • Expression

  • Cloning

Explanation

Question 2 of 28

1

An expression vector is used to _________.

Select one of the following:

  • Study the effect of overexpression of a gene on cell functions

  • Measure activities of regulatory sequences

  • Quantify the expression of a gene downstream of the regulatory sequence of interest

  • Amplify and isolate the product of a gene

Explanation

Question 3 of 28

1

Which of the following is NOT an essential component of a plasmid?

Select one of the following:

  • Ori

  • Antibiotic resistance gene

  • Multiple cloning site

  • lac operon

Explanation

Question 4 of 28

1

What would not be found in a eukaryotic expression vector?

Select one or more of the following:

  • Bacterial Ori

  • Ampicillin selectable marker

  • Eukaryotic promoter

  • MCS

  • PolyA signal

  • Neomycin - eukaryotic selectable marker

  • Transfection factor

  • luciferase gene

Explanation

Question 5 of 28

1

The expression of the _______ gene is quantified to study the activity of a regulatory sequence. In which type of vector would you find this gene?

Select one of the following:

  • luciferase - expression vector

  • luciferase - reporter vector

  • lacZ - expression vector

  • lacZ - reporter vector

Explanation

Question 6 of 28

1

Which is NOT involved in the construction of cDNA libraries?

Select one of the following:

  • reverse transcriptase

  • oligo dT primer

  • ribonuclease H

  • DNA polymerase

  • DNA ligase

Explanation

Question 7 of 28

1

What is the role of ribonuclease H in the synthesis of cDNA?

Select one of the following:

  • Degrade the mRNA template

  • Synthesize the mRNA template

  • LIgate the 2 strands of newly-synthesized DNA

  • Synthesize the first strand of cDNA from mRNA template

Explanation

Question 8 of 28

1

Which type of vector is pBluescript?

Select one of the following:

  • Expression because it tests for the expression of lacZ gene

  • Cloning because it is used to isolate DNA sequences with a built-in mechanism to select for recombinant plasmids

  • Reporter because it quantifies lacZ expression

Explanation

Question 9 of 28

1

Which outcome indicates that a colony on the pBluescript agar contains the recombinant plasmid?

Select one of the following:

  • Colony is blue

  • Colony is white

  • There is a clearing zone around the colony

Explanation

Question 10 of 28

1

Which is NOT true about enhancers sequences?

Select one of the following:

  • Always located upstream of a gene

  • Function in both 5'-3' and 3'-5' directions

  • Influence expression of genes that are physically distant from the enhancer

  • Function is not affected if location is moved

Explanation

Question 11 of 28

1

Which of the following are DNA binding motifs?

Select one or more of the following:

  • Helix-turn-helix

  • Zinc finger

  • Leucine zipper

  • Helix-loop-helix

Explanation

Question 12 of 28

1

_______ hormones bind hormone response elements (HRE) in the nucleus to upregulate target genes.

Select one of the following:

  • Steroid

  • Peptide

Explanation

Question 13 of 28

1

Epigenetics (changes in chromatin structure) is heritable and irreversible.

Select one of the following:

  • True
  • False

Explanation

Question 14 of 28

1

Position-effect variegation is the change in gene expression caused by the translocation of genes between heterochromatin and euchromatin regions.

Select one of the following:

  • True
  • False

Explanation

Question 15 of 28

1

Which does NOT result from histone acetyltransferases (HATs) activity?

Select one of the following:

  • Loosening of DNA packaging

  • Decreased gene expression

  • Easier transcriptional factor binding

  • More open chromatin

Explanation

Question 16 of 28

1

Which would lead to a decrease in gene expression?

Select one or more of the following:

  • HAT

  • HDAC

  • DNA methyltransferases

  • SWI/SNF

Explanation

Question 17 of 28

1

What is the difference in dosage compensation of Drosophila and C. elegans?

Select one of the following:

  • Drosophila: hyperactivation of male X; C. elegans: hypoactivations of both female X'es

  • Drosophila: hypoactivations of both female X'es; C. elegans: hyperactivation of male X

  • Drosophila: inactivation of a female X; C. elegans: hyperactivation of male X

  • Drosophila: hyperactivation of male X; C. elegans: inactivation of a female X

Explanation

Question 18 of 28

1

Imprinted gene is the inheritance of parental acetylation pattern.

Select one of the following:

  • True
  • False

Explanation

Question 19 of 28

1

Which does NOT result in increased mRNA stability?

Select one of the following:

  • elF4E

  • PABPI

  • Deadenylase

  • Long poly-A tail

Explanation

Question 20 of 28

1

______ removes the hairpin loop of the Pre-miRNA to form ds siRNA.

Select one of the following:

  • Drosha

  • Dicer

  • Exp5

  • Ago2

Explanation

Question 21 of 28

1

G1/S checkpoint is controlled by .
DNA damage checkpoint is controlled by .

Drag and drop to complete the text.

    Rb
    p53
    p21
    Ras

Explanation

Question 22 of 28

1

Which is NOT correct regarding cell cycle control?

Select one of the following:

  • Phosphorylation of Rb leads to growth factor independent G1/S progression.

  • A loss-of-function mutation in the p53 gene leads to a rapid accumulation of pro-cancer mutations.

  • Cyclin D is an inhibitor of Rb, which in turns is an inhibitor of E2F.

  • p53 upregulates BAX which activates the extrinsic apoptotic pathway.

Explanation

Question 23 of 28

1

The p53 gene is mutated in >50% cancers.

Select one of the following:

  • True
  • False

Explanation

Question 24 of 28

1

Which gene is the most closely-associated with chronic myelogenous leukemia?

Select one of the following:

  • bcr-abl

  • c-myc

  • Ras

  • HER2

Explanation

Question 25 of 28

1

In Burkitt's lymphoma, ______ gene is translocated to a region controlled by a strong enhancer.

Select one of the following:

  • c-myc

  • bcr-abl

  • HER2

  • Ras

Explanation

Question 26 of 28

1

Which does NOT result in growth factor independent G1/S transition?

Select one of the following:

  • Hyperphosphorylated Rb

  • Loss-of-function mutation of Rb

  • Mutation in E2F which prevents Rb binding

  • Low levels of cyclin D leading up to DNA duplication

Explanation

Question 27 of 28

1

Which of the following cellular properties is the LEAST likely to cause cancer?

Select one of the following:

  • Mutations in signalling proteins and receptors

  • Insensitive to anti-growth signals

  • Overexpression of pro-apoptotic proteins

  • Overexpression of telomerase

  • Upregulation of VEGF

  • Ability to metastasize and invade other tissues

Explanation

Question 28 of 28

1

hyperactivation results from a point mutation to Valine and GTP-independent activity.
fusion protein is closely associated with chronic myelogenous leukaemia.
is an epidermal growth factor receptor, whose oncogenic properties arises from gene amplification.
In Burkitt's lymphoma, gene is translocated to a region controlled by a strong enhancer. It is also amplified in childhood neuroblastomas.

Drag and drop to complete the text.

    Ras
    bcr-abl
    HER2
    c-myc

Explanation