If the difference between the samples we have collected is larger than we would have expect based on the standard error then we can assume one of two things:
There is no effect and we have, by chance, collected two samples that are atypical of the population from which they came. OR The two samples come from different populations but are typical of their respective parent population. In this scenario, the difference between samples represents a genuine difference between the samples (and so the null hypothesis is unlikely).
The larger the difference becomes between the two sample means:
Select one of the following: