MEDT 2410: Chapter 5 Lesson Objectives

Name the regions of the electromagnetic spectrum and the colors of the visible spectrum in order of increasing energy and decreasing wavelength.

State Beer's law in terms of absorbance and concentration and apply it in quantitative calculations.

Diagram and explain the purpose of each component of a spectrophotometer:
-Light Source

Diagram and explain the purpose of each component of a spectrophotometer:
-Monochromator

Diagram and explain the purpose of each component of a spectrophotometer:
-Sample Holder

Diagram and explain the purpose of each component of a spectrophotometer:
-Detector

Diagram and explain the purpose of each component of a spectrophotometer:
-Readout Device

Explain the term matrix and the use of a blank.

Give an example of a signal amplifier.

List the similarities and differences between prisms and diffraction grating.

Compare single and double beam.

Explain the relationship between:
-Color and Wavelength

Explain the relationship between:
-Absorbance and Concentration

Explain the relationship between:
-Absorbance and Transmission

Differentiate between light emission, reflection, and transmittance.

Colorimetry

Photometry

Spectrophotometer

λ

Frequency

Absorbance

Transmission

Describe the principle of fluorometry and the basic components of a fluorometer. Explain why fluorescence measurements may be more specific and sensitive than spectrophotometric measurements.

List the different types of chromatography and the basis for separation used in each.

Explain the principle of gas-liquid chromatography and describe several different types of detectors.

Describe the principle of electrophoresis and explain how it is commonly used in a clinical laboratory.

Describe the principle and components of a pCO2 electrode.

Discuss the principle of an ion-selective electrode technology and list the ions commonly measured by it in a clinical laboratory.

Describe common features found within automated instruments.